Improved biodiversity detection using a large-volume environmental DNA sampler with in situ filtration and implications for marine eDNA sampling strategies

Metabarcoding analysis of environmental DNA samples is a promising new tool for marine biodiversity and conservation. Typically, seawater samples are obtained using Niskin bottles and filtered to collect eDNA. However, standard sample volumes are small relative to the scale of the environment, conventional collection strategies are limited, and the filtration process is time consuming. To overcome these limitations, we developed a new large-volume eDNA sampler with in situ filtration, capable of taking up to 12 samples per deployment. We conducted three deployments of our sampler on the robotic vehicle Mesobot in the Flower Garden Banks National Marine Sanctuary in the northwestern Gulf of Mexico and collected samples from 20 to 400 m depth. We compared the large volume (~40-60 liters) samples collected by Mesobot with small volume (~2 liters) samples collected using the conventional CTD-mounted Niskin bottle approach. We sequenced the V9 region of 18S rRNA, which detects a broad range of invertebrate taxa, and found that while both methods detected biodiversity changes associated with depth, our large volume samples detected approximately 66% more taxa than the CTD small volume samples. We found that the fraction of the eDNA signal originating from metazoans relative to the total eDNA signal decreased with sampling depth, indicating that larger volume samples may be especially important for detecting metazoans in mesopelagic and deep ocean environments. We also noted substantial variability in biological replicates from both the large volume Mesobot and small volume CTD sample sets. Both of the sample sets also identified taxa that the other did not; although the number of unique taxa associated with the Mesobot samples was almost four times larger than those from the CTD samples. Large volume eDNA sampling with in situ filtration, particularly when coupled with robotic platforms, has great potential for marine biodiversity surveys, and we discuss practical methodological and sampling considerations for future applications.

Supporting Indigenous Education From a Distance

As COVID-19 swept the globe, it transformed the way people access information. This has been both challenging and metamorphic for libraries worldwide, particularly those serving indigenous people. Indigenous education has been severely impacted by the pandemic. When the pandemic swept the globe and many countries went into “lockdown,” users were not allowed to visit the physical facilities of libraries and the collections become inaccessible. This chapter is a case study about the adjustment of collection strategies to serve the needs of students in an indigenous studies program during the pandemic. This chapter aims to capture the challenges encountered at a regional-focused collection, the impacts to an indigenous studies program, the adjusting collection strategies to meet the needs of the program, and key lessons learned. The selected case is a regional-focused collection in a research library on Guam.

Collecting quantitative experimental data from a non-WEIRD population: challenges and practical recommendations from a field experiment in rural Sierra Leone

Objective Standardized pretest–posttest experimental designs with quantitative surveys are frequently applied to evaluate the effectiveness of health programs. However, this method is strongly informed by research on samples from Western, Educated, Industralized, Rich, and Democratic (WEIRD) societies and may not produce meaningful results in a distinct cultural, educational and socioeconomic context. Results This paper reports several methodological challenges encountered along the research process of collecting quantitative survey data (i.e., during recruitment, obtaining informed consent, matching pretest–posttest data and data collection) for a mixed-methods field experiment on domestic handwashing in Sierra Leone. Ethical dilemmas of certain research practices are pointed out and potential solutions or alternatives are recommended for each challenge. Analysis of these challenges highlights the importance of reflecting on the aptness of research methodologies for non-WEIRD samples. While this is not to say that quantitative surveys are not suitable in a non-WEIRD context, their employment require considerable time for extensive pilot testing, involving local interviewers and participants in designing research projects and the modification of data collection strategies.

Institutional Repositories in New Zealand: An Analysis of Coordinators’ Perspectives

<p>This study is a small scale qualitative survey of coordinators working in institutional repository development in New Zealand since critical mass was reached in 2009. It aims to summarise their opinions on the current and future roles of their repository as both a preservation archive, and a discovery resource representing their institution’s research community. The research uses narrative development techniques within the interpretivist paradigm to provide a contextual analysis of the repository’s relationship with other repositories and the National Library. It is supported by quantitative analysis of the sampled repositories’ holdings and the metadata quality with which the holdings are endowed. The analysis finds that since the establishment of New Zealand repositories, coordinators have adapted their collection strategies to encourage depositors towards Open Access publishing. These findings are placed in the context of the growth of non-mandated repository holdings and the technical infrastructure for harvesting resources, and integrating workflows with university research management systems. The results are used to discuss the goals coordinators have for improving the efficiency and visibility of their repository.</p>

Institutional Repositories in New Zealand: An Analysis of Coordinators’ Perspectives

<p>This study is a small scale qualitative survey of coordinators working in institutional repository development in New Zealand since critical mass was reached in 2009. It aims to summarise their opinions on the current and future roles of their repository as both a preservation archive, and a discovery resource representing their institution’s research community. The research uses narrative development techniques within the interpretivist paradigm to provide a contextual analysis of the repository’s relationship with other repositories and the National Library. It is supported by quantitative analysis of the sampled repositories’ holdings and the metadata quality with which the holdings are endowed. The analysis finds that since the establishment of New Zealand repositories, coordinators have adapted their collection strategies to encourage depositors towards Open Access publishing. These findings are placed in the context of the growth of non-mandated repository holdings and the technical infrastructure for harvesting resources, and integrating workflows with university research management systems. The results are used to discuss the goals coordinators have for improving the efficiency and visibility of their repository.</p>

Promoting community engagement in a pre-registration nursing programme: a qualitative study of student experiences

Aims: To offer child health student nurses a broader learning experience in practice with an autonomous choice of a volunteer placement area. To reflect the changing nature of health care and the move of care closer to home in the placement experience. To evaluate participants' experiences. Design: This study used descriptive and interpretative methods of qualitative data collection. This successive cross-sectional data collection ran from 2017 to 2020. All data were thematically analysed using Braun and Clarke's model. Methods: Data collection strategies included two focus groups (n=14) and written reflections (n=19). Results: Students identified their increased confidence, development as a professional, wider learning and community engagement. They also appreciated the relief from formal assessment of practice and the chance to focus on the experience. Conclusion: Students positively evaluated this experience, reporting a wider understanding of health and wellbeing in the community. Consideration needs to be given to risk assessments in the areas students undertake the placements and the embedding of the experience into the overall curriculum.

Simulation-based validation of activity logger data for animal behavior studies

Bio-loggers are widely used for studying the movement and behavior of animals. However, some sensors provide more data than is practical to store given experiment or bio-logger design constraints. One approach for overcoming this limitation is to utilize data collection strategies, such as non-continuous recording or data summarization that may record data more efficiently, but need to be validated for correctness. In this paper we address two fundamental questions—how can researchers determine suitable parameters and behaviors for bio-logger sensors, and how do they validate their choices? We present a methodology that uses software-based simulation of bio-loggers to validate various data collection strategies using recorded data and synchronized, annotated video. The use of simulation allows for fast and repeatable tests, which facilitates the validation of data collection methods as well as the configuration of bio-loggers in preparation for experiments. We demonstrate this methodology using accelerometer loggers for recording the activity of the small songbird Junco hyemalis hyemalis.