atomic force microscopy
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2022 ◽  
Vol 23 (2) ◽  
pp. 889
Author(s):  
Atsuya Matsui ◽  
Jean-Pierre Bellier ◽  
Takeshi Kanai ◽  
Hiroki Satooka ◽  
Akio Nakanishi ◽  
...  

The most common type of dementia, Alzheimer’s disease, is associated with senile plaques formed by the filamentous aggregation of hydrophobic amyloid-β (Aβ) in the brains of patients. Small oligomeric assemblies also occur and drugs and chemical compounds that can interact with such assemblies have attracted much attention. However, these compounds need to be solubilized in appropriate solvents, such as ethanol, which may also destabilize their protein structures. As the impact of ethanol on oligomeric Aβ assembly is unknown, we investigated the effect of various concentrations of ethanol (0 to 7.2 M) on Aβ pentameric assemblies (Aβp) by combining blue native-PAGE (BN-PAGE) and ambient air atomic force microscopy (AFM). This approach was proven to be very convenient and reliable for the quantitative analysis of Aβ assembly. The Gaussian analysis of the height histogram obtained from the AFM images was correlated with band intensity on BN-PAGE for the quantitative estimation of Aβp. Our observations indicated up to 1.4 M (8.3%) of added ethanol can be used as a solvent/vehicle without quantitatively affecting Aβ pentamer stability. Higher concentration induced significant destabilization of Aβp and eventually resulted in the complete disassembly of Aβp.


2022 ◽  
Vol 23 (2) ◽  
pp. 806
Author(s):  
Farida Akhatova ◽  
Ilnur Ishmukhametov ◽  
Gölnur Fakhrullina ◽  
Rawil Fakhrullin

The concerns regarding microplastics and nanoplastics pollution stimulate studies on the uptake and biodistribution of these emerging pollutants in vitro. Atomic force microscopy in nanomechanical PeakForce Tapping mode was used here to visualise the uptake and distribution of polystyrene spherical microplastics in human skin fibroblast. Particles down to 500 nm were imaged in whole fixed cells, the nanomechanical characterization allowed for differentiation between internalized and surface attached plastics. This study opens new avenues in microplastics toxicity research.


2022 ◽  
Author(s):  
Y.V. Kornev

Abstract. New elastomeric composites were synthesized with filler particles from rice husk products both in micro and sub-micron sizes. The obtained composites surface structure was investigated by scanning electron and atomic force microscopy. The application as a prospective strengthening sub-micron sizes filler for the composite complexes creation with high elastic and strength properties have been shown.


2022 ◽  
Author(s):  
I. Pelevin

Abstract. The study was aimed at microstructure investigations of melt-spun rare-earth intermetallic compounds using atomic force microscopy. Surface morphology of R2Fe14B (R = Y, Nd, Gd, Er) was studied with nanometric resolution. Grain structure features were discovered depending on the rare-earth element composition and quenching regime. Grain size dependence on rare earth elements' composition decreased with the metal's serial number and atomic weight. Wherein structural size dependence on quenching wheel speed had non-linear character: increase the speed from 20 to 30 m/s led to 3 times decrease of the grain size and significant surface roughness reduction.


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