Isotope Filtering, Editing

Measuring protein self-diffusion in protein–protein mixtures using a pulsed gradient spin-echo technique with WATERGATE and isotope filtering

Journal of Magnetic Resonance ◽

10.1016/j.jmr.2003.09.004 ◽

2004 ◽

Vol 166 (1) ◽

pp. 129-133 ◽

Cited By ~ 10

Author(s):

Irina V. Nesmelova ◽

Djaudat Idiyatullin ◽

Kevin H. Mayo

Keyword(s):

Spin Echo ◽

Self Diffusion ◽

Isotope Filtering ◽

Spin Echo Technique

Using delayed decoupling to attenuate residual signals in editing filters

Magnetic Resonance ◽

10.5194/mr-2-475-2021 ◽

2021 ◽

Vol 2 (1) ◽

pp. 475-487

Author(s):

Kenneth A. Marincin ◽

Indrani Pal ◽

Dominique P. Frueh

Keyword(s):

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Post Translational Modification ◽

Scalar Couplings ◽

Molecular Assemblies ◽

Nmr Studies ◽

Composite Pulse ◽

Preparation Period ◽

Isotope Filtering ◽

Nuclear Magnetic

Abstract. Isotope filtering methods are instrumental in biomolecular nuclear magnetic resonance (NMR) studies as they isolate signals of chemical moieties of interest within complex molecular assemblies. However, isotope filters suppress undesired signals of isotopically enriched molecules through scalar couplings, and variations in scalar couplings lead to imperfect suppressions, as occurs for aliphatic and aromatic moieties in proteins. Here, we show that signals that have escaped traditional filters can be attenuated with mitigated sensitivity losses for the desired signals of unlabeled moieties. The method uses a shared evolution between the detection and preceding preparation period to establish non-observable antiphase coherences and eliminates them through composite pulse decoupling. We demonstrate the method by isolating signals of an unlabeled post-translational modification tethered to an isotopically enriched protein.

Using delayed decoupling to attenuate residual signals in editing filters

10.5194/mr-2021-24 ◽

2021 ◽

Author(s):

Kenneth A. Marincin ◽

Indrani Pal ◽

Dominique P. Frueh

Keyword(s):

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Post Translational Modification ◽

Scalar Couplings ◽

Molecular Assemblies ◽

Nmr Studies ◽

Composite Pulse ◽

Preparation Period ◽

Isotope Filtering ◽

Nuclear Magnetic

Abstract. Isotope filtering methods are instrumental in biomolecular nuclear magnetic resonance (NMR) studies as they isolate signals of chemical moieties of interest within complex molecular assemblies. However, isotope filters suppress undesired signals of isotopically enriched molecules through scalar couplings, and variations in scalar couplings lead to imperfect suppressions, as occurs for aliphatic and aromatic moieties in proteins. Here, we show that signals that have escaped traditional filters can be attenuated without sensitivity losses for the desired signals of unlabeled moieties. The method uses a shared evolution between the detection and preceding preparation period to establish non-observable antiphase coherences and eliminates them through composite pulse decoupling. We demonstrate the method by isolating signals of an unlabeled post-translational modification tethered to an isotopically enriched protein.