Role of Protein Kinases on Acid-Induced Duodenal Bicarbonate Secretion in Rats

2002 ◽  
pp. 139-142
Author(s):  
Osamu Furukawa ◽  
Masahiko Hirokawa ◽  
Paul H. Guth ◽  
Eli Engel ◽  
Jonathan D. Kaunitz
Keyword(s):  
Protein Kinases ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Role of protein kinases on acid-induced duodenal bicarbonate secretion in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A156-A156
Author(s):  
O FURUKAWA ◽  
M HIROKAWA ◽  
P GUTH ◽  
E ENGEL ◽  
J KAUNITZ
Keyword(s):  
Protein Kinases ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Role of protein kinases on acid-induced duodenal bicarbonate secretion in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A156
Author(s):  
Osamu Furukawa ◽  
Masahiro Hirokawa ◽  
Paul H. Guth ◽  
Eli Engel ◽  
Jonathan D. Kaun Itz
Keyword(s):  
Protein Kinases ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Role of Protein Kinases on Acid-Induced Duodenal Bicarbonate Secretion in Rats

Pharmacology ◽  
2003 ◽  
Vol 67 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Osamu Furukawa ◽  
Masahiko Hirokawa ◽  
Paul H. Guth ◽  
Eli Engel ◽  
Jonathan D. Kaunitz
Keyword(s):  
Protein Kinases ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

scholarly journals The role of cGMP-cGKI-signaling for duodenal bicarbonate secretion

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Beate Spiessberger ◽  
Pascal Weinmeister ◽  
Franz Hofmann ◽  
Claudia Werner ◽  
Dieter Saur ◽  
...  
Keyword(s):  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Protein kinase C potentiates cAMP-stimulated mouse duodenal mucosal bicarbonate secretion in vitro

2004 ◽  
Vol 286 (5) ◽  
pp. G814-G821 ◽  
Author(s):  
Bi-Guang Tuo ◽  
Jimmy Y. C. Chow ◽  
Kim E. Barrett ◽  
Jon I. Isenberg
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Duodenal Mucosa ◽  
Bicarbonate Secretion ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Ussing Chambers ◽  
Duodenal Bicarbonate Secretion

PKC has been shown to regulate epithelial Cl- secretion in a variety of models. However, the role of PKC in duodenal mucosal bicarbonate secretion is less clear. We aimed to investigate the role of PKC in regulation of duodenal mucosal bicarbonate secretion. Bicarbonate secretion by murine duodenal mucosa was examined in vitro in Ussing chambers using a pH-stat technique. PKC isoform expression and activity were assessed by Western blotting and in vitro kinase assays, respectively. PMA (an activator of PKC) alone had no effect on duodenal bicarbonate secretion or short-circuit current ( Isc). When PMA and dibutyryl-cAMP (db-cAMP) were added simultaneously, PMA failed to alter db-cAMP-stimulated duodenal bicarbonate secretion or Isc ( P > 0.05). However, a 1-h preincubation with PMA potentiated db-cAMP-stimulated duodenal bicarbonate secretion and Isc in a concentration-dependent manner (from 10-8 to 10-5M) ( P < 0.05). PMA preincubation had no effects on carbachol- or heat-stable toxin-stimulated bicarbonate secretion. Western blot analysis revealed that PKCα, -γ, -ϵ, -θ, -μ, and -ι/λ were expressed in murine duodenal mucosa. Ro 31–8220 (an inhibitor active against PKCϵ, -α, -β, and -γ), but not Gö 6983 (an inhibitor active against PKCα, -γ, -β, and -δ), reversed the potentiating effect of PMA on db-cAMP-stimulated bicarbonate secretion. PMA also time- and concentration-dependently increased the activity of PKCϵ, an effect that was prevented by Ro 31–8220 but not Gö 6983. These results demonstrate that activation of PKC potentiates cAMP-stimulated duodenal bicarbonate secretion, whereas it does not modify basal secretion. The effect of PKC on cAMP-stimulated bicarbonate secretion is mediated by the PKCϵ isoform.

Role of prostaglandin EP4 receptors in duodenal bicarbonate secretion in rats

2003 ◽  
Vol 124 (4) ◽  
pp. A451-A452
Author(s):  
Masako Aoi ◽  
Emi Nakajima ◽  
Masato Nakashima ◽  
Koji Takeuchi
Keyword(s):  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

The role of SLC26A6 and CFTR in acid-induced and prostaglandin E2-stimulated duodenal bicarbonate secretion in vivo

2007 ◽  
Vol 45 (08) ◽  
Author(s):  
A Singh ◽  
M Sjöblom ◽  
A Krabbenhöft ◽  
B Riederer ◽  
MP Manns ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Role of Na+HCO3− cotransporter NBC1, Na+/H+ exchanger NHE1, and carbonic anhydrase in rabbit duodenal bicarbonate secretion

2000 ◽  
Vol 119 (2) ◽  
pp. 406-419 ◽  
Author(s):  
Petra Jacob ◽  
Stefanie Christiani ◽  
Heidi Rossmann ◽  
Georg Lamprecht ◽  
Dorothee Vieillard-Baron ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

scholarly journals The role of cGMP-cGKI-signaling for duodenal bicarbonate secretion

2011 ◽  
Vol 11 (S1) ◽  
Author(s):  
Beate Spiessberger ◽  
Dieter Saur ◽  
Ursula Seidler ◽  
Anurag K Singh ◽  
Robert Lukowski ◽  
...  
Keyword(s):  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion
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