G Proteins Large and Small Guanine Nucleotide Binding Proteins

2000 ◽  
pp. 27-39
Author(s):  
Thomas M. Wilkie ◽  
Michael A. White
Keyword(s):  
G Proteins ◽  
Binding Proteins ◽  
Nucleotide Binding ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins ◽  
Guanine Nucleotide Binding Proteins

scholarly journals Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3

1989 ◽  
Vol 262 (2) ◽  
pp. 403-408 ◽  
Author(s):  
F M Mitchell ◽  
S L Griffiths ◽  
E D Saggerson ◽  
M D Houslay ◽  
J T Knowler ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
G Proteins ◽  
Pertussis Toxin ◽  
Binding Proteins ◽  
Nucleotide Binding ◽  
Guanine Nucleotide ◽  
Alpha Subunits ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins ◽  
Guanine Nucleotide Binding Proteins

Considerable debate has focused on the molecular identity of the guanine-nucleotide-binding proteins (G-proteins) in adipose tissue which can be detected following pertussis-toxin-catalysed ADP-ribosylation [Rapiejko, Northup, Evans, Brown & Malbon (1986) Biochem. J. 240, 35-40; Hinsch, Rosenthal, Spicher, Binder, Gausepohl, Frank, Schultz & Joost (1988) FEBS Lett. 238, 191-196]. We have used a panel of selective anti-peptide antisera which are able to discriminate between the different pertussis-toxin-sensitive G-proteins to assess which of these are expressed in rat adipose tissue. We demonstrate that plasma membranes of rat white adipocytes contain alpha subunits corresponding to each of Gi1, Gi2 and Gi3. Furthermore, using synthetic oligonucleotides complimentary to unique regions of each of the three polypeptides, we demonstrate that the mRNAs for the three G-protein alpha subunits can also be detected in adipose tissue.

scholarly journals Changes in the guanine nucleotide-binding proteins, Gi and go , during differentiation of 3T3-L1 cells

FEBS Letters ◽  
1986 ◽  
Vol 199 (1) ◽  
pp. 103-106 ◽  
Author(s):  
Peter Gierschik ◽  
Bernice Morrow ◽  
Graeme Milligan ◽  
Charles Rubin ◽  
Allen Spiegel
Keyword(s):  
Binding Proteins ◽  
Nucleotide Binding ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins ◽  
Guanine Nucleotide Binding Proteins
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