Ribosomal DNA repeat unit polymorphism in 25 Hordeum species

1989 ◽  
Vol 78 (3) ◽  
pp. 387-392 ◽  
Author(s):  
S. J. Molnar ◽  
P. K. Gupta ◽  
G. Fedak ◽  
R. Wheatcroft
Keyword(s):  
Ribosomal Dna ◽  
Repeat Unit ◽  
Dna Repeat ◽  
Hordeum Species

Polymorphism in ribosomal DNA repeat units of 12 Hordeum species

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1124-1127 ◽  
Author(s):  
Stephen J. Molnar ◽  
George Fedak
Keyword(s):  
Ribosomal Dna ◽  
Repeat Unit ◽  
Unit Length ◽  
Intergenic Spacer ◽  
Rdna Repeat ◽  
Repeat Units ◽  
Dna Repeat ◽  
Rdna Repeat Unit ◽  
Bamhi Restriction Site ◽  
Hordeum Species

Total genomic DNA was isolated from leaves of individual accessions of 12 Hordeum species. SacI, HindIII, and BamHI restriction fragments hybridizing to the wheat rDNA probe pTA71 were compared. Thirteen rDNA repeat unit length variants were recovered, which combined to produce 11 distinct phenotypes. All rDNA repeat units had two SacI sites and no HindIII sites. The rDNA repeat units of H. secalinum, H. hrasdanicum, and H. distichum all had 3 BamHI sites, but in different configurations. The rDNA repeat units of the other species had at least 4 BamHI sites. Ladders of small hybridizing fragments in 8 of 9 of the latter accessions indicated the presence of occasional BamHI sites in some subrepeats within the intergenic spacer regions of the rDNA repeat units. Different distributions of BamHI restriction site maps within the Hordeum sections strengthen the concept of using variation in rDNA restriction sites as a taxonomic character.Key words: Hordeum, ribosomal DNA, polymorphism.

A physical map of the ribosomal DNA repeat unit of Aspergillus nidulans

Gene ◽  
1982 ◽  
Vol 20 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Robin A. Lockington ◽  
Graham G. Taylor ◽  
Michael Winther ◽  
Claudio Scazzocchio ◽  
R.Wayne Davies
Keyword(s):  
Aspergillus Nidulans ◽  
Ribosomal Dna ◽  
Physical Map ◽  
Repeat Unit ◽  
Dna Repeat

Ribosomal DNA repeat unit polymorphism in 49 Vicia species

1995 ◽  
Vol 90 (3-4) ◽  
pp. 477-486 ◽  
Author(s):  
S. N. Raina ◽  
Y. Ogihara
Keyword(s):  
Ribosomal Dna ◽  
Repeat Unit ◽  
Vicia Species ◽  
Dna Repeat

Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selections

1978 ◽  
Vol 123 (3) ◽  
pp. 371-386 ◽  
Author(s):  
Peter Philippsen ◽  
Richard A. Kramer ◽  
Ronald W. Davis
Keyword(s):  
Ribosomal Dna ◽  
Repeat Unit ◽  
Physical Size ◽  
Dna Repeat
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