Production of Recombinant Polypeptides Binding α2-Macroglobulin and Analysis of Their Ability to Bind Human Serum α2-Macroglobulin

2020 ◽  
Vol 169 (4) ◽  
pp. 478-482
Author(s):  
E. A. Bormotova ◽  
T. V. Gupalova
Keyword(s):  
Human Serum ◽  
Α2 Macroglobulin ◽  
Recombinant Polypeptides

Isolation of a zinc α2-macroglobulin from human serum

Biochemistry ◽  
1970 ◽  
Vol 9 (12) ◽  
pp. 2421-2426 ◽  
Author(s):  
Alfred F. Parisi ◽  
Bert L. Vallee
Keyword(s):  
Human Serum ◽  
Α2 Macroglobulin

Application of Crossed Radio Immunoelectrophoresis for the Demonstration of Thrombin Binding Proteins in Human Plasma

1979 ◽  
Author(s):  
L. Grimmer ◽  
L. Aukrust ◽  
P. Andersen
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Binding Proteins ◽  
Antithrombin Iii ◽  
Crossed Immunoelectrophoresis ◽  
Α2 Macroglobulin

In order to demonstrate thrombin binding proteins in human plasma in a direct way, crossed Immunoelectrophoresis (CIE) presipitates were prepared by using human plasma and anti human serum. These presipitates were incubated with 125 I-thrombin and subsequently subjected to autoradiography. Binding of thrombin was demonstrated to α2-macroglobulin only By preiricubating heat defibrinated plasma with 125I-thrombin prior to CIE and performing autoradiography without further incubation with 125I-thrombin, binding to antithrombin III was demonstrated as well. No binding of thrombin to α1-antitrypsin was observed in any of the experiments.

Purification of haptoglobin and α2-macroglobulin from human serum

1972 ◽  
Vol 49 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Charles J. Betlach ◽  
Donald E. McMillan
Keyword(s):  
Human Serum ◽  
Α2 Macroglobulin

Tartrate-resistant acid phosphatase 5B circulates in human serum in complex with α2-macroglobulin and calcium

2003 ◽  
Vol 308 (2) ◽  
pp. 320-324 ◽  
Author(s):  
Hannele Ylipahkala ◽  
Jussi M Halleen ◽  
Helena Kaija ◽  
Pirkko Vihko ◽  
H Kalervo Väänänen
Keyword(s):  
Acid Phosphatase ◽  
Human Serum ◽  
Tartrate Resistant Acid Phosphatase ◽  
Α2 Macroglobulin
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