Preliminary studies of the nature of the papain digestion products of human serum α2-macroglobulin

1964 ◽  
Vol 82 (1) ◽  
pp. 195-198 ◽  
Author(s):  
K. James ◽  
D.R. Stanworth
Biochemistry ◽  
1970 ◽  
Vol 9 (12) ◽  
pp. 2421-2426 ◽  
Author(s):  
Alfred F. Parisi ◽  
Bert L. Vallee

1945 ◽  
Vol 158 (3) ◽  
pp. 609-617
Author(s):  
Randall G. Rice ◽  
Gerald A. Ballou ◽  
Paul D. Boyer ◽  
James Murray Luck ◽  
Funston G. Lum

1979 ◽  
Author(s):  
L. Grimmer ◽  
L. Aukrust ◽  
P. Andersen

In order to demonstrate thrombin binding proteins in human plasma in a direct way, crossed Immunoelectrophoresis (CIE) presipitates were prepared by using human plasma and anti human serum. These presipitates were incubated with 125 I-thrombin and subsequently subjected to autoradiography. Binding of thrombin was demonstrated to α2-macroglobulin only By preiricubating heat defibrinated plasma with 125I-thrombin prior to CIE and performing autoradiography without further incubation with 125I-thrombin, binding to antithrombin III was demonstrated as well. No binding of thrombin to α1-antitrypsin was observed in any of the experiments.


1972 ◽  
Vol 49 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Charles J. Betlach ◽  
Donald E. McMillan

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