Purification and characterization of Bacillus thuringiensis var. tenebrionis insecticidal proteins produced in E. coli

1990 ◽  
Vol 170 (2) ◽  
pp. 665-672 ◽  
Author(s):  
S.C. MacIntosh ◽  
S.L. McPherson ◽  
F.J. Perlak ◽  
P.G. Marrone ◽  
R.L. Fuchs
Keyword(s):  
Bacillus Thuringiensis ◽  
Purification And Characterization ◽  
Insecticidal Proteins ◽  
E Coli

scholarly journals Purification and characterization of the entomocidal protoxin of Bacillus thuringiensis.

1981 ◽  
Vol 256 (6) ◽  
pp. 3000-3004
Author(s):  
L.A. Bulla ◽  
K.J. Kramer ◽  
D.J. Cox ◽  
B.L. Jones ◽  
L.I. Davidson ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Purification And Characterization

Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi

2006 ◽  
Vol 52 (7) ◽  
pp. 651-657 ◽  
Author(s):  
Luis Morales de la Vega ◽  
J Eleazar Barboza-Corona ◽  
Maria G Aguilar-Uscanga ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Sodium Dodecyl ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
Purification And Characterization ◽  
Chitinolytic Enzyme ◽  
Bacillus Thuringiensis Subsp ◽  
Sds Page ◽  
Fusarium Sp

A chitinolytic enzyme from Bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. 66 kDa by sodium dodecyl sulfate – polyacryamide gel electrophoresis (SDS–PAGE). The enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. Optimal pH and temperature were detected at 6 and 50 °C, respectively. Stability, in the absence of substrate, was observed at temperatures less than 60 °C and pH between 5 and 8. Enzyme activity was significantly inhibited by K+ and EDTA and completely inhibited by Hg2+. Purified chitinase showed lytic activity against cell walls from six phytopathogenic fungi and inhibited the mycelial growth of both Fusarium sp. and Sclerotium rolfsii. The biocontrol efficacy of the enzyme was tested in the protection of bean seeds infested with six phytopathogenic fungi.Key words: chitinase, Bacillus thuringiensis, purification, phytopathogenic fungi.

Expression, purification and characterization of human urodilatin in E. coli

2007 ◽  
Vol 55 (2) ◽  
pp. 312-318 ◽  
Author(s):  
Ziyong Sun ◽  
Wei Lu ◽  
Yanchun Tang ◽  
Jing Zhang ◽  
Junyong Chen ◽  
...  
Keyword(s):  
Purification And Characterization ◽  
E Coli

scholarly journals Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26

2004 ◽  
Vol 97 (5) ◽  
pp. 942-949 ◽  
Author(s):  
P.I. Kim ◽  
H. Bai ◽  
D. Bai ◽  
H. Chae ◽  
S. Chung ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Purification And Characterization

Purification and characterization of amylase from Bacillus thuringiensis subsp. kurstaki

Bt Research ◽  
2015 ◽  
Author(s):  
Robinson Smitha ◽  
Sreedharan Sajith ◽  
Prakasan Priji ◽  
Kizhakepowathial Nair Unni ◽  
Trikaryoor Roy ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Purification And Characterization

Expression, purification and characterization of the Cry2Aa14 toxin from Bacillus thuringiensis subsp. kenyae

Toxicon ◽  
2009 ◽  
Vol 54 (4) ◽  
pp. 519-524 ◽  
Author(s):  
Ramesh S. Hire ◽  
Ravindra D. Makde ◽  
Tanaji K. Dongre ◽  
Stanislaus F. D'souza
Keyword(s):  
Bacillus Thuringiensis ◽  
Purification And Characterization ◽  
Bacillus Thuringiensis Subsp
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