DNA synthesis in strains of Escherichia coli K12 with temperature-sensitive DNA ligase and DNA polymerase I

1974 ◽  
Vol 90 (1) ◽  
pp. 115-126 ◽  
Author(s):  
E.Bruce Konrad ◽  
Paul Modrich ◽  
I.R. Lehman
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Ligase ◽  
Temperature Sensitive ◽  
Dna Polymerase I ◽  
Escherichia Coli K12 ◽  
Polymerase I

scholarly journals The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.

Genetics ◽  
1995 ◽  
Vol 139 (4) ◽  
pp. 1483-1494 ◽  
Author(s):  
Y Cao ◽  
T Kogoma
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Reca Protein ◽  
Minor Role ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Dna Polymerase I ◽  
Recombination Repair ◽  
Polymerase I

Abstract The mechanism of recA polA lethality in Escherichia coli has been studied. Complementation tests have indicated that both the 5'-->3' exonuclease and the polymerization activities of DNA polymerase I are essential for viability in the absence of RecA protein, whereas the viability and DNA replication of DNA polymerase I-defective cells depend on the recombinase activity of RecA. An alkaline sucrose gradient sedimentation analysis has indicated that RecA has only a minor role in Okazaki fragment processing. Double-strand break repair is proposed for the major role of RecA in the absence of DNA polymerase I. The lexA(Def)::Tn5 mutation has previously been shown to suppress the temperature-sensitive growth of recA200(Ts) polA25::spc mutants. The lexA(Def) mutation can alleviate impaired DNA synthesis in the recA200(Ts) polA25::spc mutant cells at the restrictive temperature. recF+ is essential for this suppression pathway. recJ and recQ mutations have minor but significant adverse effects on the suppression. The recA200(Ts) allele in the recA200(Ts) polA25::spc lexA(Def) mutant can be replaced by delta recA, indicating that the lexA(Def)-induced suppression is RecA independent. lexA(Def) reduces the sensitivity of delta recA polA25::spc cells to UV damage by approximately 10(4)-fold. lexA(Def) also restores P1 transduction proficiency to the delta recA polA25::spc mutant to a level that is 7.3% of the recA+ wild type. These results suggest that lexA(Def) activates a RecA-independent, RecF-dependent recombination repair pathway that suppresses the defect in DNA replication in recA polA double mutants.

scholarly journals The Effects of DNA Polymerase I and Nucleotides on Ligation of Hydrogen-Bonded lambdaDNA Circles by Escherichia coli DNA Ligase.

1987 ◽  
Vol 41b ◽  
pp. 551-554 ◽  
Author(s):  
Juhani Eerik Syväoja ◽  
Ann-Marie Eklund ◽  
Curt R. Enzell ◽  
Jan-Eric Berg ◽  
Lennart Eberson
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Ligase ◽  
Dna Polymerase I ◽  
Polymerase I ◽  
Hydrogen Bonded

scholarly journals The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.

1990 ◽  
Vol 265 (23) ◽  
pp. 13878-13887 ◽  
Author(s):  
K. Bebenek ◽  
C.M. Joyce ◽  
M.P. Fitzgerald ◽  
T.A. Kunkel
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Polymerase I ◽  
Derivatives Of
Sign in / Sign up

Export Citation Format

Share Document