Fluorescent labeled α-conotoxin GI: binding interactions with the nicotinic acetylcholine receptor and monoclonal antibodies

Toxicon ◽  
1996 ◽  
Vol 34 (3) ◽  
pp. 301
Author(s):  
J.D. Ashcom ◽  
B.G. Stiles
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Nicotinic Acetylcholine ◽  
Binding Interactions

scholarly journals Characterization of α-conotoxin interactions with the nicotinic acetylcholine receptor and monoclonal antibodies

1997 ◽  
Vol 328 (1) ◽  
pp. 245-250 ◽  
Author(s):  
D. James ASHCOM ◽  
G. Bradley STILES
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Dissociation Constants ◽  
Biologically Active ◽  
Binding Assays ◽  
Nicotinic Acetylcholine ◽  
Disulphide Bonds ◽  
Conus Species

The venoms of predatory marine cone snails, Conus species, contain numerous peptides and proteins with remarkably diverse pharmacological properties. One group of peptides are the α-conotoxins, which consist of 13-19 amino acids constrained by two disulphide bonds. A biologically active fluorescein derivative of Conus geographus α-conotoxin GI (FGI) was used in novel solution-phase-binding assays with purified Torpedo californica nicotinic acetylcholine receptor (nAchR) and monoclonal antibodies developed against the toxin. The binding of FGI to nAchR or antibody had apparent dissociation constants of 10-100 nM. Structure-function studies with α-conotoxin GI analogues composed of a single disulphide loop revealed that different conformational restraints are necessary for effective toxin interactions with nAchR or antibodies.

Interaction of nicotinic acetylcholine receptor with two monoclonal antibodies recognizing different epitopes

Biochemistry ◽  
1989 ◽  
Vol 28 (10) ◽  
pp. 4222-4229 ◽  
Author(s):  
Miguel A. Chinchetru ◽  
Javier Marquez ◽  
Jose C. Garcia-Borron ◽  
David P. Richman ◽  
Marino Martinez-Carrion
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Nicotinic Acetylcholine

Monoclonal antibodies against purified nicotinic acetylcholine receptor

1979 ◽  
Vol 88 (2) ◽  
pp. 575-582 ◽  
Author(s):  
Christopher M. Gomez ◽  
David P. Richman ◽  
Phillip W. Berman ◽  
Steven A. Burres ◽  
Barry G.W. Arnason ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Nicotinic Acetylcholine
Sign in / Sign up

Export Citation Format

Share Document