Effects of pretreatment with 2,3,7,8-tetrachlorodibenzofuran on microsomal monooxygenase activity in Drosophila melanogaster

1986 ◽  
Vol 174 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Inger Hällström
Keyword(s):  
Drosophila Melanogaster ◽  
Monooxygenase Activity ◽  
Microsomal Monooxygenase

Cytosolic activation of hematin-dependent microsomal monooxygenase activity in the lung

1986 ◽  
Vol 58 ◽  
pp. 79-94 ◽  
Author(s):  
Phillip A. Dean ◽  
Allan E. Rettie ◽  
Susan M. Turnblom ◽  
Moses J. Namkung ◽  
Mont R. Juchau
Keyword(s):  
Monooxygenase Activity ◽  
Microsomal Monooxygenase

Characterization of isolated epithelial cells from rat small intestine

1982 ◽  
Vol 242 (2) ◽  
pp. G147-G155 ◽  
Author(s):  
F. Hartmann ◽  
R. Owen ◽  
D. M. Bissell
Keyword(s):  
Culture Conditions ◽  
Intestinal Lumen ◽  
Blue Dye ◽  
Isolated Cells ◽  
Monooxygenase Activity ◽  
Mucosal Cells ◽  
Ultrastructural Appearance ◽  
Microsomal Monooxygenase ◽  
A New Technique

Intestinal mucosal cells from the rat have been isolated by a new technique involving intravascular perfusion of an intestinal segment with collagenase. Detached cells were flushed from the intestinal lumen with a second perfusion circuit containing an oxygenated buffered solution with 1% bovine serum. Sequential collection of cells at intervals during the period of perfusion revealed that villus-tip cells are recovered first (after 15 min of collagenase perfusion), followed by midvillus (after 25 min) and lower villus cells (after 35 min). The isolated cells were judged intact and viable by the criteria of trypan blue dye exclusion, ultrastructural appearance, and metabolic activity. They were characterized as villus-tip, midvillus, and lower villus-crypt cells by their alkaline phosphatase and sucrase activity, glycoprotein formation, and [3H]thymidine incorporation. Microsomal monooxygenase activity was four to five times greater in villus-tip than in lower villus cells, whereas heme oxygenase exhibited a reverse gradient. The isolated cells synthesized heme and bilirubin under cell culture conditions.

Eine fluorometrische Bestimmungsmethode für mikrosomale Monooxygenase-Aktivität der Rattenleber mit Scoparon als Substrat / A Fluorometric Test for Microsomal Monooxygenase Activity in the Rat Liver with Scoparone as Substrate

1979 ◽  
Vol 34 (5-6) ◽  
pp. 481-482 ◽  
Author(s):  
Dieter Müller-Enoch ◽  
Helmut Thomas ◽  
Heinrich Ockenfels
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Polycyclic Hydrocarbons ◽  
Monooxygenase Activity ◽  
Microsomal Monooxygenase

Abstract A sensitive fluorometric test for the determination of monooxygenase activity in liver microsomes from rats is described. The assay is based on the O -demethylation of 6,7-dimethoxycoumarin (scoparone) to 7-hydroxy-6-methoxy-coumarin and 6-hydroxy-7-methoxycoumarin. Pretreatment of rats with phenobarbital or polycyclic hydrocarbons (benzo[a]pyrene, 3-methylcholanthrene) causes a significant increase in the amount of microsomal dealkylation activity.

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