Fine melting curves of chloroplast DNA of higher plants

1979 ◽  
Vol 16 (2-3) ◽  
pp. 157-164 ◽  
Author(s):  
V. Tabidze ◽  
T. Beridze
Author(s):  
Reinhold G. Herrmann ◽  
Hans-Jürgen Bohnert ◽  
Klaus V. Kowallik ◽  
Jürgen M. Schmitt

1978 ◽  
Vol 62 (6) ◽  
pp. 975-977 ◽  
Author(s):  
Martin J. Pascoe ◽  
John Ingle

1978 ◽  
Vol 79 (3) ◽  
pp. 623-630 ◽  
Author(s):  
T W James ◽  
C Jope

The DNA in isolated chloroplasts was visualized by the fluorescent probe 4'6-diamidino-2-phenylindole (DAPI). When excited with light of 360 nm, the DNA-DAPI complex fluoresces brilliantly at 450 nm. Nuclei also fluoresce but their nucleoli do not. RNase and Pronase treatment of chloroplasts did not affect the fluorescence but both pre- and posttreatment of DAPI-stained chloroplasts with DNase specifically destroyed the fluorescence. DNA-DAPI complexes in the chloroplasts show up as bright dots. These are distributed uniformly within the chloroplast except for the outer margins. The fluorescent dots can be seen at different focal levels. The number of DNA dots is roughly proportional to chloroplast area which, in turn, is a function of leaf size. The number of fluorescent dots also gave the impression that large leaves with large chloroplasts contain more chloroplast DNA than nuclear DNA.


2010 ◽  
Vol 284 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Szilvia Bajkán ◽  
Gyula Váradi ◽  
Márta Balogh ◽  
Ágota Domonkos ◽  
György B. Kiss ◽  
...  

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