Quinones as Prosthetic Groups in Membrane Electron-Transfer Proteins III: Functional Recognition of a Special Quinone Associated with the Ubiquinone–Cytochrome b-c1 Oxidoreductase of Beef Heart Mitochondria

1982 ◽  
pp. 277-283 ◽  
Author(s):  
K. MATSUURA ◽  
N.K. PACKHAM ◽  
D.M. TIEDE ◽  
P. MUELLER ◽  
P. LESLIE DUTTON
Keyword(s):  
Electron Transfer ◽  
Cytochrome B ◽  
Heart Mitochondria ◽  
Beef Heart ◽  
Electron Transfer Proteins ◽  
Beef Heart Mitochondria ◽  
Prosthetic Groups

The inhibition of mammalian mitochondrial NADU oxidation by chloramphenicol and its isomers and analogues

1968 ◽  
Vol 46 (9) ◽  
pp. 1003-1008 ◽  
Author(s):  
K. B. Freeman ◽  
D. Haldar
Keyword(s):  
Cytochrome B ◽  
Respiratory Chain ◽  
Rat Liver ◽  
Nadh Dehydrogenase ◽  
Coenzyme Q ◽  
Liver Mitochondria ◽  
Heart Mitochondria ◽  
Rat Liver Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria

Chloramphenicol and its isomers and analogues have been found to inhibit the oxidation of NADH, but not that of succinate, by beef heart mitochondria. They must therefore inhibit the NADH dehydrogenase segment of the respiratory chain. Chloramphenicol gave 50% inhibition at a concentration of 1 mM. The methylthio analogue of chloramphenicol inhibited NADH – coenzyme Q6 reductase but not NADH–ferricyanide reductase. Spectrophotometric observations suggest that these inhibitors act between NADH and flavin in coupled rat liver mitochondria and between flavin and cytochrome b in uncoupled beef heart mitochondria.

Purification of cytochrome b from Complex III of beef heart mitochondria

1985 ◽  
Vol 132 (3) ◽  
pp. 1166-1173 ◽  
Author(s):  
Hideto Nakahara ◽  
Yoshiharu Shimomura ◽  
Takayuki Ozawa
Keyword(s):  
Cytochrome B ◽  
Complex Iii ◽  
Heart Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria

scholarly journals A MACROMOLECULAR REPEATING UNIT OF MITOCHONDRIAL STRUCTURE AND FUNCTION

1964 ◽  
Vol 22 (1) ◽  
pp. 63-100 ◽  
Author(s):  
H. Fernández-Morán ◽  
T. Oda ◽  
P. V. Blair ◽  
D. E. Green
Keyword(s):  
Elementary Particle ◽  
Electron Transfer ◽  
Elementary Particles ◽  
Heart Mitochondria ◽  
Beef Heart ◽  
Electron Transfer Chain ◽  
Beef Heart Mitochondria ◽  
Subunit Organization ◽  
Transfer Chain

A repeating particle associated with the cristae and the inner membrane of the external envelope has been recognized and characterized in beef heart mitochondria by correlated electron microscopic and biochemical studies. Many thousands (ca. 104 to 105) of these particles, disposed in regular arrays, are present in a single mitochondrion. The repeating particle, called the elementary particle (EP), consists of three parts: (1) a spherical or polyhedral head piece (80 to 100 A in diameter); (2) a cylindrical stalk (about 50 A long and 30 to 40 A wide); and (3) a base piece (40 x 110 A). The base pieces of the elementary particles form an integral part of the outer dense layers of the cristae. The elementary particles can be seen in electron micrographs of mitochondria in situ, of isolated mitochondria, and of submitochondrial particles with a complete electron transfer chain. Negative staining with phosphotungstate is only one of several techniques that can be used for reproducible demonstration of the repeating particles and underlying subunit organization of mitochondrial membranes. A particulate unit containing a complete electron transfer chain can be isolated from beef heart mitochondria. The isolated unit approximates in size that of the elementary particle in situ. The molecular weight of the particle in situ is calculated to be 1.3 x 106. Evidence is presented for identifying the isolated unit with the elementary particle visualized in situ. The elementary particle of the mitochondrion is believed to be a prototype of a class of functional particles or macromolecular assemblies of similar size found in association with membranes generally.

The isolation of cytochrome b from beef heart mitochondria

1960 ◽  
Vol 2 (3) ◽  
pp. 234-238 ◽  
Author(s):  
Ralph Bomstein ◽  
Robert Goldberger ◽  
Howard Tisdale
Keyword(s):  
Cytochrome B ◽  
Heart Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria
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