Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration

FEBS Letters ◽  
2006 ◽  
Vol 581 (1) ◽  
pp. 84-90 ◽  
Author(s):  
Boris Görg ◽  
Natalia Qvartskhava ◽  
Peter Voss ◽  
Tilman Grune ◽  
Dieter Häussinger ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Tyrosine Nitration ◽  
Reversible Inhibition

scholarly journals Glutamine synthetase as a central element in hepatic glutamine and ammonia metabolism: novel aspects

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Benedikt Frieg ◽  
Boris Görg ◽  
Holger Gohlke ◽  
Dieter Häussinger
Keyword(s):  
Glutamine Synthetase ◽  
Protein Function ◽  
Nitrosative Stress ◽  
Point Mutations ◽  
Central Element ◽  
Ph Sensitive ◽  
Tyrosine Nitration ◽  
Ammonia Metabolism ◽  
Functional Consequences

Abstract Glutamine synthetase (GS) in the liver is expressed in a small perivenous, highly specialized hepatocyte population and is essential for the maintenance of low, non-toxic ammonia levels in the organism. However, GS activity can be impaired by tyrosine nitration of the enzyme in response to oxidative/nitrosative stress in a pH-sensitive way. The underlying molecular mechanism as investigated by combined molecular simulations and in vitro experiments indicates that tyrosine nitration can lead to a fully reversible and pH-sensitive regulation of protein function. This approach was also used to understand the functional consequences of several recently described point mutations of human GS with clinical relevance and to suggest an approach to restore impaired GS activity.

scholarly journals Tyrosine nitration of glutamine synthetase is increased in the prefrontal cortex of chronic immobilization stress-induced depressive mouse

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S233
Author(s):  
Jae Soon Kang ◽  
Ji Hyeong Baek ◽  
Hyeonwi Son ◽  
Hyun Joon Kim
Keyword(s):  
Prefrontal Cortex ◽  
Glutamine Synthetase ◽  
Immobilization Stress ◽  
Tyrosine Nitration ◽  
Chronic Immobilization Stress

scholarly journals Glutamine Synthetase Is a Molecular Target of Nitric Oxide in Root Nodules of Medicago truncatula and Is Regulated by Tyrosine Nitration

2011 ◽  
Vol 157 (3) ◽  
pp. 1505-1517 ◽  
Author(s):  
Paula M. Melo ◽  
Liliana S. Silva ◽  
Isa Ribeiro ◽  
Ana R. Seabra ◽  
Helena G. Carvalho
Keyword(s):  
Nitric Oxide ◽  
Glutamine Synthetase ◽  
Medicago Truncatula ◽  
Root Nodules ◽  
Molecular Target ◽  
Tyrosine Nitration

Mechanism of Fully Reversible, pH-Sensitive Inhibition of Human Glutamine Synthetase by Tyrosine Nitration

2020 ◽  
Vol 16 (7) ◽  
pp. 4694-4705
Author(s):  
Benedikt Frieg ◽  
Boris Görg ◽  
Natalia Qvartskhava ◽  
Thomas Jeitner ◽  
Nadine Homeyer ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Ph Sensitive ◽  
Tyrosine Nitration

Impact of tyrosine nitration on cellular glutamine synthetase turnover and functionality

2015 ◽  
Vol 86 ◽  
pp. S30
Author(s):  
Christiane Ott ◽  
Martin Hugo-Pereira ◽  
Walter Meinl ◽  
Nicolás Campolo ◽  
Silvina Bartesaghi ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Tyrosine Nitration

scholarly journals Lipopolysaccharide-induced tyrosine nitration and inactivation of hepatic glutamine synthetase in the rat

Hepatology ◽  
2005 ◽  
Vol 41 (5) ◽  
pp. 1065-1073 ◽  
Author(s):  
Boris Görg ◽  
Matthias Wettstein ◽  
Sabine Metzger ◽  
Freimut Schliess ◽  
Dieter Häussinger
Keyword(s):  
Glutamine Synthetase ◽  
Tyrosine Nitration

scholarly journals LPS-induced tyrosine nitration of hepatic glutamine synthetase

Hepatology ◽  
2005 ◽  
Vol 42 (2) ◽  
pp. 499-499 ◽  
Author(s):  
Boris Görg ◽  
Matthias Wettstein ◽  
Sabine Metzger ◽  
Freimut Schliess ◽  
Dieter Häussinger
Keyword(s):  
Glutamine Synthetase ◽  
Tyrosine Nitration

Evaluation of single-electron movies of glutamine synthetase molecules

1983 ◽  
Vol 41 ◽  
pp. 280-281
Author(s):  
W. Kunath ◽  
E. Zeitler ◽  
M. Kessel
Keyword(s):  
Electron Microscope ◽  
Glutamine Synthetase ◽  
Electron Irradiation ◽  
Structural Damage ◽  
Structural Changes ◽  
Single Electron ◽  
Continuous Series ◽  
Digital Recording ◽  
Recording Device ◽  
Low Exposure

The features of digital recording of a continuous series (movie) of singleelectron TV frames are reported. The technique is used to investigate structural changes in negatively stained glutamine synthetase molecules (GS) during electron irradiation and, as an ultimate goal, to look for the molecules' “undamaged” structure, say, after a 1 e/Å2 dose.The TV frame of fig. la shows an image of 5 glutamine synthetase molecules exposed to 1/150 e/Å2. Every single electron is recorded as a unit signal in a 256 ×256 field. The extremely low exposure of a single TV frame as dictated by the single-electron recording device including the electron microscope requires accumulation of 150 TV frames into one frame (fig. lb) thus achieving a reasonable compromise between the conflicting aspects of exposure time per frame of 3 sec. vs. object drift of less than 1 Å, and exposure per frame of 1 e/Å2 vs. rate of structural damage.

Rapid and Reversible Inhibition of Brain Aromatase Activity

2001 ◽  
Vol 13 (1) ◽  
pp. 63-73 ◽  
Author(s):  
J. Balthazart ◽  
M. Baillien ◽  
G. F. Ball
Keyword(s):  
Aromatase Activity ◽  
Reversible Inhibition ◽  
Brain Aromatase
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