Development of monoclonal antibodies to Rift Valley Fever Virus and their application in antigen detection and indirect immunofluorescence

2018 ◽  
Vol 460 ◽  
pp. 36-44 ◽  
Author(s):  
Claudia Mroz ◽  
Kristina M. Schmidt ◽  
Sven Reiche ◽  
Martin H. Groschup ◽  
Martin Eiden
Keyword(s):  
Monoclonal Antibodies ◽  
Indirect Immunofluorescence ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Antigen Detection ◽  
Fever Virus ◽  
Valley Fever

scholarly journals Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus

2019 ◽  
Vol 4 (7) ◽  
pp. 1231-1241 ◽  
Author(s):  
Qihui Wang ◽  
Tong Ma ◽  
Yan Wu ◽  
Zhihai Chen ◽  
Hui Zeng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Human Monoclonal Antibodies ◽  
Valley Fever

scholarly journals Potent neutralization of Rift Valley fever virus by human monoclonal antibodies through fusion inhibition

2021 ◽  
Vol 118 (14) ◽  
pp. e2025642118
Author(s):  
Nathaniel S. Chapman ◽  
Haiyan Zhao ◽  
Nurgun Kose ◽  
Jonna B. Westover ◽  
Birte Kalveram ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Antigenic Sites ◽  
Fusion Inhibition ◽  
Neutralizing Capacity ◽  
Fusion Loop

Rift Valley fever virus (RVFV), an emerging arboviral and zoonotic bunyavirus, causes severe disease in livestock and humans. Here, we report the isolation of a panel of monoclonal antibodies (mAbs) from the B cells of immune individuals following natural infection in Kenya or immunization with MP-12 vaccine. The B cell responses of individuals who were vaccinated or naturally infected recognized similar epitopes on both Gc and Gn proteins. The Gn-specific mAbs and two mAbs that do not recognize either monomeric Gc or Gn alone but recognized the hetero-oligomer glycoprotein complex (Gc+Gn) when Gc and Gn were coexpressed exhibited potent neutralizing activities in vitro, while Gc-specific mAbs exhibited relatively lower neutralizing capacity. The two Gc+Gn–specific mAbs and the Gn domain A-specific mAbs inhibited RVFV fusion to cells, suggesting that mAbs can inhibit the exposure of the fusion loop in Gc, a class II fusion protein, and thus prevent fusion by an indirect mechanism without direct fusion loop contact. Competition-binding analysis with coexpressed Gc/Gn and mutagenesis library screening indicated that these mAbs recognize four major antigenic sites, with two sites of vulnerability for neutralization on Gn. In experimental models of infection in mice, representative mAbs recognizing three of the antigenic sites reduced morbidity and mortality when used at a low dose in both prophylactic and therapeutic settings. This study identifies multiple candidate mAbs that may be suitable for use in humans against RVFV infection and highlights fusion inhibition against bunyaviruses as a potential contributor to potent antibody-mediated neutralization.

Generation and application of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein NP and glycoproteins Gn and Gc

2013 ◽  
Vol 159 (3) ◽  
pp. 535-546 ◽  
Author(s):  
Susanne Jäckel ◽  
Martin Eiden ◽  
Malte Dauber ◽  
Anne Balkema-Buschmann ◽  
Alejandro Brun ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

MONOCLONAL ANTIBODIES TO IDENTIFY ZINGA VIRUS AS RIFT VALLEY FEVER VIRUS

The Lancet ◽  
1983 ◽  
Vol 321 (8325) ◽  
pp. 641 ◽  
Author(s):  
J.M. Meegan ◽  
J.P. Digoutte ◽  
C.J. Peters ◽  
R.E. Shope
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

scholarly journals Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization

mAbs ◽  
2010 ◽  
Vol 2 (3) ◽  
pp. 275-284 ◽  
Author(s):  
Raquel Martín-Folgar ◽  
Gema Lorenzo ◽  
Hani Boshra ◽  
Javier Iglesias ◽  
Francisco Mateos ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Dna Immunization ◽  
Valley Fever

scholarly journals Generation and Characterization of Monoclonal Antibodies Against Rift Valley Fever Virus Nucleoprotein

2013 ◽  
Vol 60 ◽  
pp. 24-30 ◽  
Author(s):  
J. M. Fafetine ◽  
A. Domingos ◽  
S. Antunes ◽  
A. Esteves ◽  
J. T. Paweska ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever
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