Impact of insulin concentration and mode of FSH addition on the in vitro survival and development of isolated bovine preantral follicles

2016 ◽  
Vol 86 (4) ◽  
pp. 1137-1145 ◽  
Author(s):  
R. Rossetto ◽  
M.V.A. Saraiva ◽  
M.P. Bernuci ◽  
G.M. Silva ◽  
I.R. Brito ◽  
...  
Keyword(s):  
Insulin Concentration ◽  
Preantral Follicles ◽  
Survival And Development ◽  
In Vitro Survival

scholarly journals Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles

2010 ◽  
Vol 30 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Valdevane R. Araújo ◽  
Cleidson M. Gomes da Silva ◽  
Deborah M. Magalhães ◽  
Gerlane Modesto da Silva ◽  
Sônia N. Báo ◽  
...  
Keyword(s):  
Oocyte Diameter ◽  
Cortical Tissue ◽  
Minimum Essential Medium ◽  
Preantral Follicles ◽  
Morphogenetic Protein ◽  
Transmission Electron ◽  
Survival And Development ◽  
Survival And Growth ◽  
In Vitro Survival

This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.

Fraction of Auxemma oncocalyx and Oncocalyxone A Affects the In Vitro Survival and Development of Caprine Preantral Follicles Enclosed in Ovarian Cortical Tissue

2016 ◽  
Vol 23 (5) ◽  
pp. 307-313 ◽  
Author(s):  
Johanna Leiva-Revilla ◽  
Laritza Ferreira Lima ◽  
Simone Vieira Castro ◽  
Claudio Cabral Campello ◽  
Valdevane Rocha Araújo ◽  
...  
Keyword(s):  
Cortical Tissue ◽  
Preantral Follicles ◽  
Survival And Development ◽  
In Vitro Survival

In vitro survival and development of goat preantral follicles in two different oxygen tensions

2010 ◽  
Vol 117 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
C.M.G. Silva ◽  
M.H.T. Matos ◽  
G.Q. Rodrigues ◽  
L.R. Faustino ◽  
L.C. Pinto ◽  
...  
Keyword(s):  
Preantral Follicles ◽  
Survival And Development ◽  
Oxygen Tensions ◽  
In Vitro Survival

Interaction between Estradiol and Follicle-Stimulating Hormone Promotes in vitro Survival and Development of Caprine Preantral Follicles

2010 ◽  
Vol 191 (3) ◽  
pp. 240-247 ◽  
Author(s):  
I.B. Lima-Verde ◽  
M.H.T. Matos ◽  
M.V.A. Saraiva ◽  
J.B. Bruno ◽  
S.B. Tenório ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Preantral Follicles ◽  
Survival And Development ◽  
In Vitro Survival ◽  
Stimulating Hormone

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

2017 ◽  
Vol 5 (9) ◽  
Author(s):  
Gildas Tetaping Mbemya ◽  
Denise Damasceno Guerreiro ◽  
Nathalie Jiatsa Donfack ◽  
Luciana Mascena Silva ◽  
Luis Alberto Vieira ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Preantral Follicles ◽  
Survival And Development ◽  
In Vitro Survival

Moment of Addition of LH to the Culture Medium Improves In Vitro Survival and Development of Secondary Goat Pre-antral Follicles

2010 ◽  
Vol 46 (4) ◽  
pp. 579-584 ◽  
Author(s):  
CMG Silva ◽  
SV Castro ◽  
LR Faustino ◽  
GQ Rodrigues ◽  
IR Brito ◽  
...  
Keyword(s):  
Culture Medium ◽  
Antral Follicles ◽  
Survival And Development ◽  
In Vitro Survival

Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture

2010 ◽  
Vol 191 (5) ◽  
pp. 414-421 ◽  
Author(s):  
J.B. Bruno ◽  
J.J.H. Celestino ◽  
I.B. Lima-Verde ◽  
M.H.T. Matos ◽  
L.F. Lima ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Vasoactive Intestinal Peptide ◽  
Preantral Follicles ◽  
In Vitro Tissue Culture ◽  
Survival And Development

scholarly journals Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

2012 ◽  
Vol 32 (4) ◽  
pp. 361-367 ◽  
Author(s):  
Isabel B. Lima-Verde ◽  
Maria H.T. Matos ◽  
Juliana J.H. Celestino ◽  
Rafael Rossetto ◽  
Khesller P.O. Name ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Follicle Stimulating Hormone ◽  
Primordial Follicles ◽  
Preantral Follicles ◽  
Ultrastructural Studies ◽  
Survival And Development ◽  
Survival And Growth ◽  
Development In Vitro ◽  
Stimulating Hormone

We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles.

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