Cost and Practice Size Limit Adoption of EHRs

2006 ◽  
Vol 39 (14) ◽  
pp. 63
Author(s):  
JANE NEFF ROLLINS
Keyword(s):  
2011 ◽  
Vol 158 (1-2) ◽  
pp. 154-160 ◽  
Author(s):  
Takayuki Suzuki ◽  
Tomomi Sasaki ◽  
Koyori Yano ◽  
Fuminori Sakurai ◽  
Kenji Kawabata ◽  
...  

2016 ◽  
Vol 195 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Axel W. Fischer ◽  
Enrica Bordignon ◽  
Stephanie Bleicken ◽  
Ana J. García-Sáez ◽  
Gunnar Jeschke ◽  
...  

1999 ◽  
Vol 67 (10) ◽  
pp. 920-922 ◽  
Author(s):  
Boye K. Ahlborn ◽  
Robert W. Blake

1999 ◽  
Vol 112 (12) ◽  
pp. 2033-2041
Author(s):  
J.J. Ludtke ◽  
G. Zhang ◽  
M.G. Sebestyen ◽  
J.A. Wolff

Although the entry of DNA into the nucleus is a crucial step of non-viral gene delivery, fundamental features of this transport process have remained unexplored. This study analyzed the effect of linear double stranded DNA size on its passive diffusion, its active transport and its NLS-assisted transport. The size limit for passive diffusion was found to be between 200 and 310 bp. DNA of 310–1500 bp entered the nuclei of digitonin treated cells in the absence of cytosolic extract by an active transport process. Both the size limit and the intensity of DNA nuclear transport could be increased by the attachment of strong nuclear localization signals. Conjugation of a 900 bp expression cassette to nuclear localization signals increased both its nuclear entry and expression in microinjected, living cells.


2013 ◽  
Vol 63 (614) ◽  
pp. 459-460 ◽  
Author(s):  
Pieter van den Hombergh ◽  
Stephen Campbell

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