Uridine diphosphate glucose synthase from calf liver. Determinants of enzyme activity in vitro

Biochemistry ◽  
1975 ◽  
Vol 14 (25) ◽  
pp. 5445-5450 ◽  
Author(s):  
Peter J. Roach ◽  
Kenneth R. Warren ◽  
Daniel E. Atkinson
Keyword(s):  
Enzyme Activity ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucose ◽  
Diphosphate Glucose

scholarly journals In vitro synthesis of particulate glycogen from uridine diphosphate glucose

1969 ◽  
Vol 132 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Armando J. Parodi ◽  
José Mordoh ◽  
Clara R. Krisman ◽  
Luis F. Leloir
Keyword(s):  
Uridine Diphosphate ◽  
In Vitro Synthesis ◽  
Uridine Diphosphate Glucose ◽  
Diphosphate Glucose

scholarly journals Studies on glycogen synthesis in pigeon liver homogenates. Glycogen synthesis from glucose monophosphates and uridine diphosphate glucose

1967 ◽  
Vol 105 (2) ◽  
pp. 515-519 ◽  
Author(s):  
V. N. Nigam
Keyword(s):  
Time Course ◽  
Initial Rate ◽  
Glycogen Synthesis ◽  
Uridine Diphosphate ◽  
Cytoplasmic Fraction ◽  
Uridine Diphosphate Glucose ◽  
Diphosphate Glucose ◽  
Liver Homogenates ◽  
Pigeon Liver ◽  
Glycogen Formation

Comparative time-course studies of glycogen synthesis from glucose 6-phosphate, glucose 1-phosphate and UDP-glucose show that glucose 1-phosphate forms glycogen at an initial rate faster than that obtained with glucose 6-phosphate and UDP-glucose. After 5min. the rates from glucose monophosphates are considerably slower. 2,4-Dinitrophenol decreases glycogen synthesis from both glucose monophosphates, whereas arsenate and EDTA increase glycogen synthesis from glucose 1-phosphate and inhibit the reaction from glucose 6-phosphate, galactose and galactose 1-phosphate. Mitochondria-free pigeon liver cytoplasmic fraction forms less glycogen from glucose monophosphates than does the whole homogenate. 2-Deoxyglucose 6-phosphate inhibits glycogen synthesis from glucose monophosphates. Glycogen formation from UDP-glucose is relatively unaffected by dinitrophenol, by arsenate, by EDTA, by 2-deoxyglucose 6-phosphate and by the removal of mitochondria from the whole homogenate.

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