scholarly journals Interleukin 10 inhibits transforming growth factor-beta (TGF-beta) synthesis required for osteogenic commitment of mouse bone marrow cells

1994 ◽  
Vol 124 (4) ◽  
pp. 569-577 ◽  
Author(s):  
P Van Vlasselaer ◽  
B Borremans ◽  
U van Gorp ◽  
JR Dasch ◽  
R De Waal-Malefyt
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Interleukin 10 ◽  
Mouse Bone Marrow ◽  
Adherent Cells ◽  
Tgf Beta ◽  
Osteoprogenitor Cell ◽  
Mineralized Matrix ◽  
Beta 2 ◽  
Marrow Cells

Interleukin 10 (IL-10) suppressed TGF-beta synthesis in mouse bone marrow cultures. Coincidingly, IL-10 down-regulated the production of bone proteins including alkaline phosphatase (ALP), collagen and osteocalcin, and the formation of mineralized extracellular matrix. The mAb 1D11.16 which neutralizes TGF-beta 1 and TGF-beta 2, induced suppressive effects comparable to IL-10 when administered before the increase of cell proliferation in the culture. It appears that mainly TGF-beta 1 plays a role in this system since (a) TGF-beta 2 levels were undetectable in supernatants from osteogenic cultures, (b) no effect was observed when the anti-TGF-beta 2 neutralizing mAb 4C7.11 was added and (c) the suppressive effect of IL-10 could be reversed by adding exogenous TGF-beta 1. It is unlikely that TGF-beta 1 modulates osteogenic differentiation by changing the proliferative potential of marrow cells since 1D11.16 did not affect [3H]thymidine ([3H]TdR) incorporation or the number of fibroblast colony forming cells (CFU-F) which harbor the osteoprogenitor cell population. Furthermore, 1D11.16 did not alter [3H]TdR uptake by the cloned osteoprogenitor cell lines MN7 and MC3T3. Light and scanning electron microscopy showed that IL-10 and 1D11.16 induced comparable morphological changes in the marrow cultures. Control cultures contained flat adherent cells embedded in a mineralized matrix. In contrast, IL-10 and 1D11.16 treated cultures were characterized by round non-adherent cells and the absence of a mineralized matrix. In this study, the mechanism by which IL-10 suppresses the osteogenic differentiation of mouse bone marrow was identified as inhibition of TGF-beta 1 production which is essential for osteogenic commitment of bone marrow cells.

Interleukin-10 inhibits RANKL-mediated expression of NFATc1 in part via suppression of c-Fos and c-Jun in RAW264.7 cells and mouse bone marrow cells

Bone ◽  
2007 ◽  
Vol 41 (4) ◽  
pp. 592-602 ◽  
Author(s):  
Saad Gad-Kamel Mohamed ◽  
Eiji Sugiyama ◽  
Kouichiro Shinoda ◽  
Hirofumi Taki ◽  
Hiroyuki Hounoki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Interleukin 10 ◽  
Raw264.7 Cells ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals Brahmarasayana protects against Ethyl methanesulfonate or Methyl methanesulfonate induced chromosomal aberrations in mouse bone marrow cells

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Kanive Parashiva Guruprasad ◽  
Advait Subramanian ◽  
Vikram Jeet Singh ◽  
Raghavendra Sudheer Kumar Sharma ◽  
Puthiya Mundyat Gopinath ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberrations ◽  
Bone Marrow Cells ◽  
Ethyl Methanesulfonate ◽  
Methyl Methanesulfonate ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals Hepatocyte-like cells from directed differentiation of mouse bone marrow cells in vitro1

2005 ◽  
Vol 26 (4) ◽  
pp. 469-476 ◽  
Author(s):  
Xiao-lei SHI ◽  
Yu-dong QIU ◽  
Qiang LI ◽  
Ting XIE ◽  
Zhang-hua ZHU ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Directed Differentiation ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Induction of micronuclei in mouse bone marrow cells: An evaluation of nucleoside analogues used in the treatment of AIDS

1991 ◽  
Vol 18 (3) ◽  
pp. 168-183 ◽  
Author(s):  
Marcia D. Phillips ◽  
Bruce Nascimbeni ◽  
Raymond R. Tice ◽  
Michael D. Shelby ◽  
A. A. Van Zeeland
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Nucleoside Analogues ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

J-LEAPS Vaccines Mature Mouse Bone Marrow Cells to Dendritic Cells-1 (DC1) which can Deliver Protective Immunity

2010 ◽  
Vol 135 ◽  
pp. S32
Author(s):  
Patricia Taylor ◽  
Gary Koski ◽  
Erin Bailey ◽  
Daniel Zimmerman ◽  
Ken S. Rosenthal
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Protective Immunity ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Mature Mouse ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Acute cytogenetic effects of tyramine and MTCAs on mouse bone marrow cells in vivo by the micronucleus test

1990 ◽  
Vol 240 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Kimiko Fujie ◽  
Junko Nishi ◽  
Mieko Wada ◽  
Sakan Maeda ◽  
Taketoshi Sugiyama
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Mouse Bone Marrow ◽  
Cytogenetic Effects ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Genotoxic Evaluation of Sodium Fluoride and Sodium Perborate in Mouse Bone Marrow Cells

2005 ◽  
Vol 74 (3) ◽  
pp. 566-572 ◽  
Author(s):  
N. Velazquez-Guadarrama ◽  
E. Madrigal-Bujaidar ◽  
D. Molina ◽  
G. Chamorro
Keyword(s):  
Bone Marrow ◽  
Sodium Fluoride ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Sodium Perborate ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals CONTRIBUTION OF A THYMIC HUMORAL FACTOR TO THE DEVELOPMENT OF AN IMMUNOLOGICALLY COMPETENT POPULATION FROM CELLS OF MOUSE BONE MARROW

1971 ◽  
Vol 134 (3) ◽  
pp. 786-800 ◽  
Author(s):  
Myra Small ◽  
Nathan Trainin
Keyword(s):  
Bone Marrow ◽  
Lymphoid Tissue ◽  
Host Response ◽  
Bone Marrow Cells ◽  
Immune Reactivity ◽  
Mouse Bone Marrow ◽  
Graft Versus Host ◽  
Peripheral Lymphoid Tissue ◽  
Marrow Cells

The hypothesis that cells located in mouse bone marrow can acquire immunological competence by a process that involves interaction with a noncellular component of the thymus was tested using an in vitro assay of graft-versus-host reactivity as a criterion of cell competence. When suspensions of C57BL bone marrow cells were incubated in thymus extract and injected into mice incapable of inducing a response in the graft-versus-host assay as a result of neonatal thymectomy, or adult thymectomy plus irradiation, or because of genetic similarity with the (C3H x C57BL)F1 tissue used for challenge in the assay, competent cells were recovered from the spleens of the injected mice. The reactive cells were shown to be of bone marrow origin since immune reactivity was related to the genetic makeup of the bone marrow cells rather than that of the intermediate recipients. A thymic factor was involved in the process leading to immune reactivity by these cells, as bone marrow cells incubated in xenogeneic or syngeneic thymic extracts induced a graft-versus-host response after passage through nonresponsive mice, whereas incubation of bone marrow cells in xenogeneic lymph node or spleen extracts or in culture medium only did not lead to subsequent reactivity. Participation of peripheral lymphoid tissue seemed essential in this process since bone marrow cells tested directly after exposure to thymic extract failed to induce a graft-versus-host response. C57BL bone marrow cells exposed to thymus extract and cultured together with fragments of (C3H x C57BL)F1 spleen tissue in vitro were competent to induce a graft-versus-host response; thus, these components would seem to be sufficient as well as necessary for the immunodifferentiation process leading to graft-versus-host activity. It is concluded that one step in the process by which bone marrow cells acquire competence vis-a-vis the graft-versus-host response depends upon a thymic agent that is noncellular and extractable, and that another stage in this process is under the influence of components found within the peripheral lymphoid tissue environment. It is suggested that differentiation of precursor cells to competence could occur by progressive development of the cells in separate compartments of the lymphoid system.

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