scholarly journals IN VITRO INDUCTION OF A PRIMARY RESPONSE TO THE DINITROPHENYL DETERMINANT

1970 ◽  
Vol 131 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Shraga Segal ◽  
Amiela Globerson ◽  
Michael Feldman ◽  
Joseph Haimovich ◽  
Michael Sela
Keyword(s):  
Antibody Response ◽  
Normal Mouse ◽  
Culture Medium ◽  
Primary Response ◽  
Primary Antibody ◽  
Primary Antibody Response ◽  
T4 Phage ◽  
In Vitro Induction ◽  
Stimulation Of

Primary antibody response against the dinitrophenyl group has been elicited in vitro after the stimulation of normal mouse spleen explants with 2,4-dinitrophenyl (DNP)-hemocyanin or α-DNP-poly-L-lysine (PLL). Antibodies were detected in the culture medium by the inactivation of DNP-T4 phage. The specificity of the reaction was manifested by the lack of the capacity of the medium to inactivate the unmodified bacteriophage and by the inhibition of the inactivation of DNP-T4 with DNP-lysine.

scholarly journals INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES

1969 ◽  
Vol 130 (4) ◽  
pp. 895-906 ◽  
Author(s):  
Samuel Strober
Keyword(s):  
Antibody Response ◽  
Thoracic Duct ◽  
Salmonella Typhi ◽  
Primary Response ◽  
Primary Antibody ◽  
Spleen Cells ◽  
Normal Cells ◽  
Duct Cells ◽  
Primary Antibody Response

Thoracic duct cells and spleen cells were tested for their ability to restore the primary antibody response of X-irradiated rats to bovine serum albumin (BSA), sheep red blood cells (SRBC), horse spleen femtin (HSF), and Salmonella typhi flagella. Spleen cells were at least as efficient as thoracic duct cells in restoring the response to BSA, HSF, and Salmonella typhi flagella. In further experiments thoracic duct cells lacking large dividing lymphocytes were tested for their ability to restore the primary response. Large lymphocytes were eliminated by the in vitro incubation of thoracic duct cells for 24 hr at 37°C or by treatment of thoracic duct cell donors with the mitotic inhibitor vinblastine sulfate 24 hr prior to cannulation of the thoracic duct. Experiments with SRBC show that incubated cells and cells from vinblastine-treated donors are as efficient as normal cells in restoring the primary antibody response. On the other hand, experiments with HSF and Salmonella typhi flagella show that incubated cells and cells from vinblastine-treated donors are about five times less efficient than normal cells in restoring the response. Normal thoracic duct cells were more efficient than incubated cells but less efficient than cells from vinblastine-treated donors in restoring the early response to BSA. The experimental findings indicate that the classes of thoracic duct lymphocytes which initiate the primary antibody response to SRBC differ from the classes which initiate the response to HSF and Salmonella typhi flagella, or BSA.

scholarly journals HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT

1964 ◽  
Vol 120 (6) ◽  
pp. 987-1005 ◽  
Author(s):  
Donald A. Rowley ◽  
Frank W. Fitch
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Antibody Formation ◽  
Sheep Erythrocyte ◽  
Passive Immunization ◽  
Primary Antibody ◽  
Secondary Response ◽  
Primary Antibody Response

Passive immunization of rats with homologous anti-sheep erythrocyte serum markedly inhibited the primary antibody response to various doses of sheep erythrocytes. Inhibition was "specific" and apparently produced by either "19S" or "7S" antibody to the antigen. Passive immunization inhibited splenic hyperplasia associated with the primary antibody response. Passive immunization 24 hours after active immunization effectively inhibited the primary antibody response. The markedly suppressive effect of specific antibody on the primary antibody response contrasted sharply with the absence of this effect on the secondary response. Antigen-antibody complexes formed in vitro elicited no measurable primary antibody response but did elicit a high secondary response. Exposure of normal spleen cells to the antibody in vivo or in vitro suppressed their response to the antigen in x-irradiated recipients. In contrast, cells from previously immunized animals transferred to x-irradiated animals produced antibody in the presence of passively given antibody. Thus, "potential antibody-forming cells" from normal animals were unresponsive to the antigen in the presence of specific antibody, while "antibody-forming cells" from previously immunized animals responded to the antigen in the presence of antibody. Presumably, antibody actively produced in small quantities by a few antibody-forming cells might inhibit antibody formation by potential antibody-forming cells. Confirmation of this suggestion was obtained by showing that some animals initially injected with small doses of antigen failed to produce measurable antibody to subsequent injections of larger doses of the antigen. Low doses of antigen capable of inducing unresponsiveness produced no measurable circulating antibody, but these doses did produce increased numbers of plaque-forming (antibody-releasing) cells in spleens of rats. Thus, the formation of specific antibody may provide a homeostatic or "feed-back" mechanism which controls or limits production of specific antibody to the portion of the antibody-forming system previously stimulated by the antigen. This mechanism may account in part for immunological unresponsiveness produced in certain other related experimental systems.

scholarly journals STUDIES ON ANTIBODY PRODUCTION

1963 ◽  
Vol 117 (6) ◽  
pp. 1063-1074 ◽  
Author(s):  
Thomas F. O'Brien ◽  
Albert H. Coons
Keyword(s):  
Lymph Node ◽  
Antibody Response ◽  
Antibody Production ◽  
Culture Medium ◽  
Popliteal Lymph Node ◽  
Cell Multiplication ◽  
Measurable Effect ◽  
In Vitro System ◽  
Stimulation Of

Incorporation of 5-bromodeoxyuridine (BUDR) in the culture medium for the 2nd, 3rd, or 4th day after in vitro antigen stimulation of rabbit popliteal lymph node fragments suppressed the in vitro anamnestic antibody response described previously. Before or after this 3-day period, BUDR had no measurable effect. The results suggest that the antibody response in this in vitro system depends upon cell multiplication during this period.

Transfer to Neonatal Chickens of the Primary Antibody Response initiated in vitro

Nature ◽  
1963 ◽  
Vol 200 (4907) ◽  
pp. 696-697
Author(s):  
EUGENE F. GOLD ◽  
ALBERT A. BENEDICT
Keyword(s):  
Antibody Response ◽  
Primary Antibody ◽  
Primary Antibody Response
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