scholarly journals STUDIES ON TYPHUS FEVER

1931 ◽  
Vol 53 (4) ◽  
pp. 493-497 ◽  
Author(s):  
Hans Zinsser ◽  
M. Ruiz Castaneda
Keyword(s):  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Active Immunization ◽  
Rocky Mountain Spotted Fever ◽  
Typhus Fever

Guinea pigs can be immunized against Mexican typhus virus by peritoneal injections of formalinized Rickettsia material, provided sufficient amounts of the organisms are used. Our results in this respect are analogous to those of Spencer and Parker with carbolized virus of Rocky Mountain spotted fever. The Rickettsia suspensions appear to possess considerable toxicity. We do not wish to be misunderstood as implying that the results in guinea pigs offer anything more than a demonstration of the principle of active immunization with killed Rickettsiae. Application to man will have to be worked out, and preliminary to this, we are now attempting to apply the methods to a limited number of monkeys.

scholarly journals MORPHOLOGICAL STRUCTURE OF RICKETTSIAE

1943 ◽  
Vol 77 (4) ◽  
pp. 355-358 ◽  
Author(s):  
Harry Plotz ◽  
Joseph E. Smadel ◽  
Thomas F. Anderson ◽  
Leslie A. Chambers
Keyword(s):  
Vaccinia Virus ◽  
Q Fever ◽  
Spotted Fever ◽  
Morphological Structure ◽  
Rocky Mountain ◽  
Rocky Mountain Spotted Fever ◽  
Dense Granules ◽  
Endemic Typhus ◽  
Typhus Fever

The morphological structures of the rickettsiae of epidemic and endemic typhus fever, Rocky Mountain spotted fever, and Q fever are similar to one another and to certain bacteria. The rickettsial organisms in common with the elementary bodies of vaccinia virus and all bacteria would appear to have a limiting membrane which surrounds a substance that seems to be protoplasmic in nature; numbers of dense granules are embedded in the inner protoplasm.

scholarly journals LOCALIZATION OF ANTIGEN IN TISSUE CELLS

1950 ◽  
Vol 91 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Albert H. Coons ◽  
John C. Snyder ◽  
F. Sargent Cheever ◽  
Edward S. Murray
Keyword(s):  
Spotted Fever ◽  
Rocky Mountain ◽  
Virus Antigen ◽  
Rocky Mountain Spotted Fever ◽  
Infected Monkey ◽  
Tissue Sections ◽  
Epidemic Typhus ◽  
Cotton Rats ◽  
Antigenic Material ◽  
Typhus Fever

Rickettsiae of epidemic typhus fever and Rocky Mountain spotted fever have been microscopically localized and identified in smears of exudates and tissue sections from infected cotton rats by means of homologous antibody labelled with fluorescein. Epidemic typhus has also been identified in smears from single infected human body lice. Mumps virus antigen has been microscopically localized in the parotid of the experimentally infected monkey by the same method. The antigenic material, probably active virus, was found in the cytoplasm of the acinar cells. Such infected acini were scattered irregularly throughout the gland. Some antigen could be seen in the lumens of the parotid ducts and small amounts were present in the cytoplasm of the epithelial cells lining the ducts.

scholarly journals IMMUNITY STUDIES OF ROCKY MOUNTAIN SPOTTED FEVER

1923 ◽  
Vol 38 (5) ◽  
pp. 605-626 ◽  
Author(s):  
Hideyo Noguchi
Keyword(s):  
Experimental Infection ◽  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Rocky Mountain Spotted Fever ◽  
Refrigerator Temperature ◽  
Considerable Loss ◽  
Immune Rabbit

Freshly prepared mixtures of spotted fever virus and immune rabbit serum in neutral or superneutral proportions confer complete immunity on guinea pigs. The mixtures undergo a considerable loss in immunizing power when heated to 60°C. for 20 minutes, but are still capable, if used in sufficient quantity, of conferring a degree of immunity on the vaccinated animal such that a subsequent experimental infection is rendered less severe and non-fatal. Unheated mixtures which had been preserved in the refrigerator at 4°C. for a period of 32 days still retained a certain degree of immunizing property. The virus alone, or mixed with normal rabbit serum, when allowed to die out by prolonged preservation at refrigerator temperature, or when killed either by heating at 60°C. for 20 minutes or by chemicals (chloroform, ether, xylene) does not induce immunity in guinea pigs.

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