scholarly journals Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage ϕ29 DNA mimic protein p56

2013 ◽  
Vol 41 (13) ◽  
pp. 6761-6773 ◽  
Author(s):  
José Ignacio Baños-Sanz ◽  
Laura Mojardín ◽  
Julia Sanz-Aparicio ◽  
José M. Lázaro ◽  
Laurentino Villar ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase

Crystal Structure of Family 5 Uracil-DNA Glycosylase Bound to DNA

2007 ◽  
Vol 373 (4) ◽  
pp. 839-850 ◽  
Author(s):  
Hiromichi Kosaka ◽  
Jun Hoseki ◽  
Noriko Nakagawa ◽  
Seiki Kuramitsu ◽  
Ryoji Masui
Keyword(s):  
Crystal Structure ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase

scholarly journals SMUG2 DNA glycosylase from Pedobacter heparinus as a new subfamily of the UDG superfamily

2017 ◽  
Vol 474 (6) ◽  
pp. 923-938 ◽  
Author(s):  
Panjiao Pang ◽  
Ye Yang ◽  
Jing Li ◽  
Zhong Wang ◽  
Weiguo Cao ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Excision Repair ◽  
Phylogenetic Analyses ◽  
Dna Glycosylase ◽  
Amino Acid Residues ◽  
Uracil Dna Glycosylase ◽  
Important Amino Acid ◽  
Repair Mechanisms ◽  
Ap Sites ◽  
Base Excision

Base deamination is a common type of DNA damage that occurs in all organisms. DNA repair mechanisms are essential to maintain genome integrity, in which the base excision repair (BER) pathway plays a major role in the removal of base damage. In the BER pathway, the uracil DNA glycosylase superfamily is responsible for excising the deaminated bases from DNA and generates apurinic/apyrimidinic (AP) sites. Using bioinformatics tools, we identified a family 3 SMUG1-like DNA glycoyslase from Pedobacter heparinus (named Phe SMUG2), which displays catalytic activities towards DNA containing uracil or hypoxanthine/xanthine. Phylogenetic analyses show that SMUG2 enzymes are closely related to family 3 SMUG1s but belong to a distinct branch of the family. The high-resolution crystal structure of the apoenzyme reveals that the general fold of Phe SMUG2 resembles SMUG1s, yet with several distinct local structural differences. Mutational studies, coupled with structural modeling, identified several important amino acid residues for glycosylase activity. Substitution of G65 with a tyrosine results in loss of all glycosylase activity. The crystal structure of the G65Y mutant suggests a potential misalignment at the active site due to the mutation. The relationship between the new subfamily and other families in the UDG superfamily is discussed. The present study provides new mechanistic insight into the molecular mechanism of the UDG superfamily.

Crystal Structure of a Family 4 Uracil-DNA Glycosylase from Thermus thermophilus HB8

2003 ◽  
Vol 333 (3) ◽  
pp. 515-526 ◽  
Author(s):  
Jun Hoseki ◽  
Akihiro Okamoto ◽  
Ryoji Masui ◽  
Takehiko Shibata ◽  
Yorinao Inoue ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Thermus Thermophilus ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Thermus Thermophilus Hb8

scholarly journals Crystal Structure of the Vaccinia Virus Uracil-DNA Glycosylase in Complex with DNA

2015 ◽  
Vol 290 (29) ◽  
pp. 17923-17934 ◽  
Author(s):  
Wim P. Burmeister ◽  
Nicolas Tarbouriech ◽  
Pascal Fender ◽  
Céline Contesto-Richefeu ◽  
Christophe N. Peyrefitte ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Vaccinia Virus ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase

scholarly journals Crystal structure of family 4 uracil-DNA glycosylase fromSulfolobus tokodaiiand a function of tyrosine 170 in DNA binding

FEBS Letters ◽  
2015 ◽  
Vol 589 (19PartB) ◽  
pp. 2675-2682 ◽  
Author(s):  
Akito Kawai ◽  
Shigesada Higuchi ◽  
Masaru Tsunoda ◽  
Kazuo T. Nakamura ◽  
Yuriko Yamagata ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase

scholarly journals Crystal structure of vaccinia virus uracil-DNA glycosylase reveals dimeric assembly

2007 ◽  
Vol 7 (1) ◽  
pp. 45 ◽  
Author(s):  
Norbert Schormann ◽  
Alexei Grigorian ◽  
Alexandra Samal ◽  
Raman Krishnan ◽  
Lawrence DeLucas ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Vaccinia Virus ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase

scholarly journals Crystal structure and mutational analysis of human uracil-DNA glycosylase: Structural basis for specificity and catalysis

Cell ◽  
1995 ◽  
Vol 80 (6) ◽  
pp. 869-878 ◽  
Author(s):  
Clifford D Mol ◽  
Andrew S Arvai ◽  
Geir Slupphaug ◽  
Bodil Kavli ◽  
Ingrun Alseth ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Mutational Analysis ◽  
Dna Glycosylase ◽  
Structural Basis ◽  
Uracil Dna Glycosylase
Sign in / Sign up

Export Citation Format

Share Document