As for the requirement of size and energy distribution of focal spot in the laser detection area, this paper designs a laser beam-shaping system of flow cytometry. The 640nm red diode laser and 488nm blue solid laser which are arranged vertically are used as the excitation source. The emergent ray of two lasers is combined by beam splitter, and the combined beam is focused on the screen through two orthogonal cylindrical lens. The focal spot is compressed by doublet lens which is also used to eliminate chromatic aberration of focal spot. Applying ZEMAX software to optimize the whole optical system, the simulated results meet fully the actual experiment for individual blood cells, and it has certain theoretical significance for actual design of optical system about flow cytometry.
Tailored laser beam shaping for efficient and accurate microstructuring