THE EFFECTS OF SOLUBILIZATION PROCEDURES ON THE RELEASE AND MOLECULAR STATE OF ACETYLCHOLINESTERASE FROM ELECTRIC ORGAN TISSUE

1974 ◽  
Vol 23 (6) ◽  
pp. 1177-1187 ◽  
Author(s):  
Y. Dudai ◽  
I. Silman
Keyword(s):  
Electric Organ ◽  
Molecular State ◽  
Organ Tissue

scholarly journals A beta-D-galactoside binding protein from electric organ tissue of Electrophorus electricus.

1975 ◽  
Vol 72 (4) ◽  
pp. 1383-1387 ◽  
Author(s):  
V. I. Teichberg ◽  
I. Silman ◽  
D. D. Beitsch ◽  
G. Resheff
Keyword(s):  
Binding Protein ◽  
Electric Organ ◽  
Electrophorus Electricus ◽  
Organ Tissue

scholarly journals Carbohydrate patterns of the pure cholinergic synapse of Torpedo electric organ: a cytochemical and immunocytochemical electron microscopic approach.

1992 ◽  
Vol 40 (4) ◽  
pp. 513-521 ◽  
Author(s):  
G Egea ◽  
J Marsal
Keyword(s):  
Electric Organ ◽  
T Antigen ◽  
Neuraminic Acid ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Organ Tissue ◽  
Staining Techniques ◽  
Torpedo Electric Organ ◽  
Pre Treatment ◽  
Synaptic Structures

Using post-embedding gold staining techniques, we investigated the ultrastructural distribution of terminal sugars and carbohydrate chains located at the pure cholinergic electric organ tissue of Torpedo marmorata. Neither alpha-N-acetylgalactosamine (GalNAc)-specific lectins (DBA, SBA, HPA) nor monoclonal antibodies (MAb) recognizing Tn (Gal-NAc alpha-O-Ser/Thr; MAb Cu-1) and sialyl-Tn epitopes (NeuAc alpha 2,6GalNAc alpha-O-Ser/Thr; MAb B72.3 and OSM-10) were capable of labeling any of the synaptic structures. The absence of gold labeling was likewise noted with UEA-I (L-fucose) and with PNA (T-antigen, Gal beta 1,3GalNAc alpha). After neuraminidase pre-treatment of ultra-thin sections, PNA labeling was rendered evident, indicating the presence of neuraminic acid-masked T-antigen. Certain synaptic vesicles were labeled for neuraminic acid (LFA) and for N-acetyllactosamine (DSA), whereas others were not labeled at all. Gold labeling with LFA, RCA-I (beta-galactose), and DSA in the membrane infoldings of the dorsal face of the electrocyte was visualized. As noted above, the PNA reaction was revealed only after pre-treatment with neuraminidase. Dorsal (non-synaptic) basal lamina were reactive with DSA, whereas the synaptic portion was likewise labeled with LFA and RCA-I. Finally, RCA-I labeling was noted in the Schwann cell nucleus. Comparisons between these results and those described at the neuromuscular junction were made.

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