scholarly journals Heterologous expression of nonribosomal peptide synthetases inB. subtilis: construction of a bi-functionalB. subtilis/E. colishuttle vector system

2002 ◽  
Vol 216 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Sascha Doekel ◽  
Katrin Eppelmann ◽  
Mohamed A Marahiel
Keyword(s):  
Heterologous Expression ◽  
Vector System ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases

scholarly journals One pathway, two cyclic pentapeptides: heterologous expression of BE-18257 A-C and pentaminomycins A-E from Streptomyces cacaoi CA-170360

2020 ◽  
Author(s):  
Fernando Román-Hurtado ◽  
Marina Sánchez-Hidalgo ◽  
Jesús Martín ◽  
Francisco Javier Ortiz-López ◽  
Daniel Carretero-Molina ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Binding Protein ◽  
Biosynthetic Gene Cluster ◽  
Protein Family ◽  
Biosynthetic Gene ◽  
Penicillin Binding Protein ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases

1.AbstractThe strain Streptomyces cacaoi CA-170360 produces the cyclic pentapeptides pentaminomycins A-E and BE-18257 A-C, two families of cyclopeptides synthesized by two nonribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the nonribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.

scholarly journals Nonprocessive [2 + 2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases

2012 ◽  
Vol 109 (15) ◽  
pp. 5681-5686 ◽  
Author(s):  
A. Chhabra ◽  
A. S. Haque ◽  
R. K. Pal ◽  
A. Goyal ◽  
R. Rai ◽  
...  
Keyword(s):  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases

scholarly journals Core Assembly Mechanism of Quinocarcin/SF-1739: Bimodular Complex Nonribosomal Peptide Synthetases for Sequential Mannich-type Reactions

2013 ◽  
Vol 20 (12) ◽  
pp. 1523-1535 ◽  
Author(s):  
Tomoshige Hiratsuka ◽  
Kento Koketsu ◽  
Atsushi Minami ◽  
Shunsuke Kaneko ◽  
Chiaki Yamazaki ◽  
...  
Keyword(s):  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Assembly Mechanism

Kinetics Profiling of Gramicidin S Synthetase A, a Member of Nonribosomal Peptide Synthetases

Biochemistry ◽  
2014 ◽  
Vol 53 (50) ◽  
pp. 7983-7989 ◽  
Author(s):  
Xun Sun ◽  
Hao Li ◽  
Jonas Alfermann ◽  
Henning D. Mootz ◽  
Haw Yang
Keyword(s):  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Gramicidin S

scholarly journals The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases

1999 ◽  
Vol 6 (8) ◽  
pp. 493-505 ◽  
Author(s):  
Torsten Stachelhaus ◽  
Henning D Mootz ◽  
Mohamed A Marahiel
Keyword(s):  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases
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