scholarly journals Cloning and Characterization of a cDNA Encoding a Novel Extracellular Peroxidase from Trametes versicolor

1999 ◽  
Vol 65 (3) ◽  
pp. 1343-1347 ◽  
Author(s):  
Patrick J. Collins ◽  
Margaret M. O’Brien ◽  
Alan D. W. Dobson
Keyword(s):  
Trametes Versicolor ◽  
Cdna Sequence ◽  
White Rot ◽  
Transcript Levels ◽  
Extracellular Peroxidase ◽  
Low Concentrations ◽  
Lignin Peroxidases

ABSTRACT The white rot basidiomycete Trametes versicolorsecretes a large number of peroxidases which are believed to be involved in the degradation of polymeric lignin. These peroxidases have been classified previously as lignin peroxidases or manganese peroxidases (MnP). We have isolated a novel extracellular peroxidase-encoding cDNA sequence from T. versicolor CU1, the transcript levels of which are repressed by low concentrations of Mn2+ and induced by nitrogen and carbon but not induced in response to a range of stresses which have been reported to induce MnP expression.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF THREE ORGANOTIN(IV) CARBOXYLATE OF ENT-KAURENOIC ACID: ANTIFUNGAL ACTIVITY AGAINST TRAMETES VERSICOLOR (L.: FR) PILÀT

2019 ◽  
Vol 16 (32) ◽  
pp. 912-918
Author(s):  
P. QUINTERO-RINCÓN ◽  
B. FONTAL-RIVERA ◽  
R. CONTRERAS ◽  
Y. FONSECA ◽  
J. VELÁSQUEZ-GIL
Keyword(s):  
Trametes Versicolor ◽  
Forest Products ◽  
White Rot ◽  
Wood Preservation ◽  
Dilution Method ◽  
Forest Products Industry ◽  
Kaurenoic Acid ◽  
Standing Trees ◽  
Anti Fungal

The forest products industry has a very important role in the development toward a sustainable, biobased society. However, many fungi that deteriorate the main structural components of wood, such as cellulose, hemicellulose and lignin, are able to cause decay in standing trees, leading to significant losses in forest output due to impacts on biomass production. Trametes versicolor (L.: Fr) Pilát is a fungus able to attack the trees by saprotrophic and parasitic life strategies, causing white-rot of wood species, a serious problem for the industry that is focused on wood and its uses. The objectives of the work were the synthesis of organotin(IV) carboxylate of a natural product (ent-kaurenoic acid), characterization of the structures and anti-fungal evaluation against T. versicolor, as a contribution of new alternatives for the wood preservation. The synthesis of organotin(IV) complexes derived from the diterpene tetracyclic ent-kaur-16-en-19-oic acid (ent-kaurenoic acid, KA) gave three new complexes potentially active against T. versicolor. The complexes were characterized by FTIR and NMR spectroscopy. The biological activity was evaluated by a gel dilution method with superficialplate inoculation, using 60 and 120 μg/mL concentration, for the KA and the three organotin(IV) complexes. A considerably higher bioactivity was observed to the 120 μg/mL concentration, and for the complex with greater molecular weight.

Synthesis and characterization of immobilized white-rot fungus Trametes versicolor in sol–gel ceramics

2015 ◽  
Vol 77 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Wan Nurul Izyani Wan Mohd Zawawi ◽  
Azmi Fadziyana Mansor ◽  
Nurul Sakinah Othman ◽  
Nur Atikah Mohidem ◽  
Nik Ahmad Nizam Nik Malek ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Sol Gel ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthesis And Characterization

scholarly journals A Manganese(II)-Dependent Extracellular Peroxidase from the White-Rot Fungus Trametes versicolor.

1987 ◽  
Vol 41b ◽  
pp. 762-765 ◽  
Author(s):  
Tomas Johansson ◽  
Per Olof Nyman ◽  
Jacek Koziol ◽  
Franz Müller ◽  
Michael Sjöström
Keyword(s):  
Trametes Versicolor ◽  
White Rot ◽  
White Rot Fungus ◽  
Extracellular Peroxidase

scholarly journals Characterization of Bioethanol Production from Hexoses and Xylose by the White Rot Fungus Trametes versicolor

2011 ◽  
Vol 5 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Rasika L. Kudahettige ◽  
Marie Holmgren ◽  
Peter Imerzeel ◽  
Anita Sellstedt
Keyword(s):  
Trametes Versicolor ◽  
White Rot ◽  
White Rot Fungus ◽  
Bioethanol Production

scholarly journals Differential Regulation of mnp2, a New Manganese Peroxidase-Encoding Gene from the Ligninolytic Fungus Trametes versicolor PRL 572

2002 ◽  
Vol 68 (4) ◽  
pp. 2077-2080 ◽  
Author(s):  
Tomas Johansson ◽  
Per Olof Nyman ◽  
Daniel Cullen
Keyword(s):  
Reverse Transcriptase ◽  
Manganese Peroxidase ◽  
Trametes Versicolor ◽  
Differential Regulation ◽  
White Rot ◽  
Structural Domains ◽  
Transcript Levels ◽  
Response Elements ◽  
Plant Peroxidases ◽  
Encoding Gene

ABSTRACT A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology.

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