Preparation, Crystallization, and Preliminary X-Ray Diffraction Study of Mutant Carboxypeptidase T Bearing the Primary Specificity Pocket and the Active-Site Loop of Carboxypeptidase B

2020 ◽  
Vol 65 (6) ◽  
pp. 900-902
Author(s):  
V. Kh. Akparov ◽  
G. E. Konstantinova ◽  
V. I. Timofeev ◽  
I. P. Kuranova ◽  
I. G. Khaliullin
Keyword(s):  
Diffraction Study ◽  
Active Site ◽  
X Ray Diffraction ◽  
Carboxypeptidase B ◽  
X Ray ◽  
Carboxypeptidase T ◽  
Primary Specificity

Crystallization and preliminary X-ray diffraction study of porcine carboxypeptidase B

2015 ◽  
Vol 60 (3) ◽  
pp. 367-369 ◽  
Author(s):  
V. Kh. Akparov ◽  
V. I. Timofeev ◽  
I. P. Kuranova
Keyword(s):  
Diffraction Study ◽  
X Ray Diffraction ◽  
Carboxypeptidase B ◽  
X Ray

scholarly journals Study of the Interaction of Sorption and Catalytic Centers in Carboxypeptidase T by X-ray Analysis

Crystals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1088
Author(s):  
Valerij Akparov ◽  
Vladimir Timofeev ◽  
Inna Kuranova ◽  
Ilias Khaliullin
Keyword(s):  
Substrate Specificity ◽  
Transition State ◽  
Kinetic Data ◽  
Carboxypeptidase B ◽  
Catalytic Center ◽  
X Ray ◽  
Thermoactinomyces Vulgaris ◽  
Distant Homolog ◽  
Carboxypeptidase T ◽  
Primary Specificity

Carboxypeptidase T (CPT; EC 3.4.17.18) from Thermoactinomyces vulgaris is a distant homolog of the highly specific pancreatic carboxypeptidase B; but has a broad substrate specificity; the source of which remains unclear. A previous study of the structural bases of the substrate specificity of CPT using stable sulfamoyl analogs of the transition state of the elimination of leucine; phenylalanine; arginine; and glutamic acid; showed that the binding of the C-terminal residue of the substrate to the primary selectivity pocket of CPT leads to a change in the distance between Zn2+ and the sulfur atom. This value is related to the efficiency of catalysis of the corresponding substrate or the inhibition constant of the corresponding stable analog of the transition state. In this work; we obtained crystallographic and kinetic data of the complex of CPT with N-sulfamoyl-L-valine; confirming the effect of the binding of the ligand’s side group by the primary specificity pocket of CPT on the structure of the catalytic center; which can explain the unusual substrate specificity of CPT.

Volume and X-ray diffraction study of terephthal-bis-4,n-decylaniline (TBDA)

1986 ◽  
Vol 47 (1) ◽  
pp. 133-138 ◽  
Author(s):  
D. Guillon ◽  
A. Skoulios ◽  
J.J. Benattar
Keyword(s):  
Diffraction Study ◽  
X Ray Diffraction ◽  
X Ray

X-ray diffraction study on uniformly oriented quasicrystals

1987 ◽  
Vol 48 (8) ◽  
pp. 1357-1361 ◽  
Author(s):  
F. Dénoyer ◽  
G. Heger ◽  
M. Lambert ◽  
J.M. Lang ◽  
P. Sainfort
Keyword(s):  
Diffraction Study ◽  
X Ray Diffraction ◽  
X Ray

AN X-RAY DIFFRACTION STUDY OF THE THERMAL MOTION OF ATOMS IN β-TIN

1980 ◽  
Vol 41 (C1) ◽  
pp. C1-145-C1-146 ◽  
Author(s):  
B. Greenberg ◽  
G. M. Rothberg
Keyword(s):  
Diffraction Study ◽  
Thermal Motion ◽  
X Ray Diffraction ◽  
X Ray

Synchrotron X-ray diffraction study on the cation distribution in quaternary Li-Fe-Mn spinel oxides

2007 ◽  
Vol 2007 (suppl_26) ◽  
pp. 477-482
Author(s):  
W. Nowicki ◽  
J. Darul ◽  
P. Piszora ◽  
C. Baehtz ◽  
E. Wolska
Keyword(s):  
Diffraction Study ◽  
Cation Distribution ◽  
X Ray Diffraction ◽  
X Ray ◽  
Spinel Oxides
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