Detection of ribosomal RNA genes in soybean, Glycine max (L.) Merr., by in situ hybridization

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1091-1095 ◽  
Author(s):  
Halina Skorupska ◽  
Marc C. Albertsen ◽  
Kim D. Langholz ◽  
Reid G. Palmer
Keyword(s):  
In Situ Hybridization ◽  
Glycine Max ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Single Pair ◽  
Glycine Max L ◽  
Labeled Probe ◽  
Rna Genes

A biotinylated maize rRNA probe was hybridized to soybean nuclei. Hybridization was detected by using a streptavidin horseradish peroxidase biotin system. The procedure used enabled detection of heterologous complementary 18S and 25S rRNA coding genes in soybean. In diploid cultivars 'Hark' and 'Lincoln' a single pair of satellited chromosomes was present and two binding sites were detected at interphase. In plants trisomic for the satellited chromosome, three sites were observed, and in tetraploid nuclei, four sites were seen. The in situ hybridization results indicated that, for ribosomal RNA genes, Glycine max behaves as a diploid. We discuss the possibility of loss of a pair of satellited chromosomes in the evolution of soybean.Key words: biotin-labeled probe, rRNA genes, ploidy, Glycine max.

Ribosomal RNA genes and the substructure of nucleolar organizing regions in Lilium

1984 ◽  
Vol 26 (2) ◽  
pp. 158-166 ◽  
Author(s):  
L. von Kalm ◽  
D. R. Smyth
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Relative Size ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Gene Number ◽  
Nucleolar Organizing Regions ◽  
Band Size ◽  
Rna Genes

We have examined the location and organization of nucleolar organizing regions (NORs) in the species Lilium henryi (with four NORs per diploid cell), L. longiflorum, and L. speciosum (each with six NORs), and the hybrids Lilium × 'Black Beauty' (five NORs) and Lilium × parkmannii (eight NORs). The relative number of genes in individual NORs was assayed by in situ hybridization using in vitro labelled ribosomal RNA (rRNA). An estimate of their relative transcriptional activity was obtained by scoring silver band size over the constriction. There was no clear correlation between gene number and activity at specific NORs. Rather, gene number correlated quite well with the relative size of heterochromatin usually found adjacent to NOR constrictions and stained by an acid-banding method. It is possible that many but a variable fraction of the rRNA genes in Lilium NORs are held inactive in nucleolar heterochromatin.Key words: ribosomal RNA genes, Lilium, nucleolus, in situ hybridization, heterochromatin, silver banding.

Localization of 18S+28S and 5S ribosomal RNA genes in the dog by fluorescence in situ hybridization

1997 ◽  
Vol 78 (3-4) ◽  
pp. 231-235 ◽  
Author(s):  
A. Mäkinen ◽  
C. Zijlstra ◽  
N.A. de Haan ◽  
C.H.M. MellInk ◽  
A.A. Bosma
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
5S Ribosomal Rna ◽  
Rna Genes

scholarly journals Karyotype Analysis of Three Juniperus Species using Fluorescence in situ Hybridization (FISH) with Two Ribosomal RNA Genes

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Katsuya Nagano ◽  
Hideyuki Matoba ◽  
Kouhei Yonemura ◽  
Yasushi Matsuda ◽  
Tatsurou Murata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Karyotype Analysis ◽  
Ribosomal Rna Genes ◽  
Rna Genes
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