scholarly journals Contribution of α-Adrenergic and β-Adrenergic Stimulation to Ischemia-Induced Glucose Transporter (GLUT) 4 and GLUT1 Translocation in the Isolated Perfused Rat Heart

1999 ◽  
Vol 84 (12) ◽  
pp. 1407-1415 ◽  
Author(s):  
Silvia Egert ◽  
Ngoc Nguyen ◽  
Markus Schwaiger
Keyword(s):  
Rat Heart ◽  
Glucose Transporter ◽  
Adrenergic Stimulation ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart ◽  
Glut 4

Greater glycogen utilization during β1- than β2-adrenergic receptor stimulation in the isolated perfused rat heart

2007 ◽  
Vol 293 (6) ◽  
pp. E1828-E1835 ◽  
Author(s):  
Patrick McConville ◽  
Edward G. Lakatta ◽  
Richard G. Spencer
Keyword(s):  
Rat Heart ◽  
Adrenergic Receptor ◽  
Left Ventricular ◽  
Rate Pressure Product ◽  
Lv Function ◽  
Adrenergic Stimulation ◽  
Functional Responses ◽  
Perfused Rat Heart ◽  
Phosphorylation Potential ◽  
Isolated Perfused Rat Heart

Differences in energy metabolism during β1- and β2-adrenergic receptor (AR) stimulation have been shown to translate to differences in the elicited functional responses. It has been suggested that differential access to glycogen during β1- compared with β2-AR stimulation may influence the peak functional response and modulation of the response during sustained adrenergic stimulation. Interleaved 13C- and 31P-NMR spectroscopy was used during β1- and β2-AR stimulation at matched peak workload (2.5 times baseline) in the isolated perfused rat heart to monitor glycogen levels, phosphorylation potential, and intracellular pH. Simultaneous measurements of left ventricular (LV) function [LV developed pressure (LVDP)], heart rate (HR), and rate-pressure product (RPP = LVDP × HR) were also performed. The heart was perfused under both substrate-free (SF) conditions and with exogenous glucose (G). The greater glycogenolysis was observed during β1- than β2-AR stimulation with G (54% vs. 38% reduction, P = 0.006) and SF (92% vs. 79% reduction, P = 0.04) perfusions. The greater β1-AR-mediated glycogenolysis was correlated with greater ability to sustain the initial contractile response. However, with SF perfusion, the duration of this ability was limited: excessive early glycogen depletion caused an earlier decline in LVDP and phosphorylation potential during β1- than β2-AR stimulation. Therefore, endogenous glycogen stores are depleted earlier and to a greater extent, despite a slightly weaker overall inotropic response, during β1- than β2-AR stimulation. These findings are consistent with β1-AR-specific PKA-dependent glycogen phosphorylase kinase signaling.

Global ischemic duration and reperfusion function in the isolated perfused rat heart

Resuscitation ◽  
2004 ◽  
Vol 62 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Brian S. Palmer ◽  
Mersiha Hadziahmetovic ◽  
Timothy Veci ◽  
Mark G. Angelos
Keyword(s):  
Rat Heart ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart ◽  
Ischemic Duration
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