scholarly journals ISOLATION OF APICAL MEMBRANES FROM HORMONE-SENSITIVE RAT COLONIC CRYPT CELLS

1989 ◽  
Vol 26 (3) ◽  
pp. 280-280
Author(s):  
S Walther ◽  
P Heinz-Erian ◽  
A Roscher ◽  
Y Shin-Zoulek ◽  
W Stolz ◽  
...  
Keyword(s):  
Colonic Crypt ◽  
Hormone Sensitive ◽  
Apical Membranes ◽  
Rat Colonic Crypt ◽  
Crypt Cells

The basolateral Ca 2+ -dependent K + channel in rat colonic crypt cells

1997 ◽  
Vol 435 (2) ◽  
pp. 267-272 ◽  
Author(s):  
M. S. Nielsen ◽  
R. Warth ◽  
M. Bleich ◽  
B. Weyand ◽  
R. Greger
Keyword(s):  
K Channel ◽  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Cells

Serotonin Activates Electrolyte Transport via 5-HT2AReceptor in Rat Colonic Crypt Cells

1997 ◽  
Vol 230 (2) ◽  
pp. 437-441 ◽  
Author(s):  
Yuko Imada-Shirakata ◽  
Tohru Kotera ◽  
Shunji Ueda ◽  
Minoru Okuma
Keyword(s):  
Electrolyte Transport ◽  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Cells

Thromboxane A2Receptor Linked with the Ca2+Pathway in Rat Colonic Crypt Cells

1999 ◽  
Vol 258 (3) ◽  
pp. 708-712 ◽  
Author(s):  
Akira Ikari ◽  
Hideki Sakai ◽  
Takahiro Sato ◽  
Noriaki Takeguchi
Keyword(s):  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Cells

scholarly journals Mobilization of Intracellular Ca2+ by Thromboxane A2 Does Not Affect Ca2+-Activated K+ Channels in Rat Colonic Crypt Cells.

2000 ◽  
Vol 50 (3) ◽  
pp. 389-393 ◽  
Author(s):  
Tomoyuki Suzuki ◽  
Hideki Sakai ◽  
Akira Ikari ◽  
Noriaki Takeguchi
Keyword(s):  
Thromboxane A2 ◽  
Colonic Crypt ◽  
K Channels ◽  
Rat Colonic Crypt ◽  
Crypt Cells

Effect of the flavonol quercetin on membrane conductances in rat colonic crypt cells

2004 ◽  
Vol 503 (1-3) ◽  
pp. 31-34 ◽  
Author(s):  
Rainer Cermak ◽  
Siegfried Wolffram ◽  
Gisela Kuhn
Keyword(s):  
Colonic Crypt ◽  
Membrane Conductances ◽  
Rat Colonic Crypt ◽  
Crypt Cells

Steroid specificity of the putative DHB receptor: evidence that the receptor is not 11βHSD

1998 ◽  
Vol 275 (1) ◽  
pp. E124-E131 ◽  
Author(s):  
Karen E. Sheppard ◽  
Karen Khoo ◽  
Zygmunt S. Krozowski ◽  
Kevin X. Z. Li
Keyword(s):  
Steroid Receptors ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Rat Colon ◽  
Colonic Crypt ◽  
Binding Assays ◽  
Ovary Cells ◽  
High Affinity ◽  
Steroid Binding ◽  
Crypt Cells

Recently, we identified a novel putative nuclear receptor in colonic crypt cells distinct from both mineralocorticoid receptor and glucocorticoid receptor, with high affinity for 11-dehydrocorticosterone (11-DHB) (33). In the present study, competitive nuclear binding assays demonstrated that this site has a unique steroid binding specificity that distinguishes it from other steroid receptors. Western blot analysis showed the presence of 11β-hydroxysteroid dehydrogenase-2 (11βHSD2) but not 11βHSD1 in colonic crypt cells and showed that 11βHSD2 was present in the nuclear pellet. Differences in steroid specificity between the putative DHB receptor and inhibition of 11βHSD activity indicate that binding is not to the enzyme. Furthermore, modified Chinese hamster ovary cells transfected with the 11βHSD2 gene express nuclear 11βHSD2 but not a nuclear DHB binding site. In conclusion, these data support the existence of a novel nuclear DHB receptor in rat colon that is distinct from the classic steroid receptors and from both 11βHSD1 and 11βHSD2.

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