Periodic sequences over finite fields, constructed by classical cyclotomic classes and generalized cyclotomic classes, have good pseudo-random properties. The linear complexity of a period sequence plays a fundamental role in the randomness of sequences. In this paper, we construct a new family of quaternary generalized cyclotomic sequences with order [Formula: see text] and length [Formula: see text], which generalize the sequences constructed by Ke et al. in 2012. In addition, we determine its linear complexity using cyclotomic theory. The conclusions reveal that these sequences have high linear complexity, which means they can resist linear attacks.