scholarly journals Yields of tubulin paracrystals, vinblastine-crystals, induced in unfertilized and fertilized sea urchin eggs in the presence of D2O.

1984 ◽  
Vol 9 (1) ◽  
pp. 45-52 ◽  
Author(s):  
T. Choku Takahashi ◽  
Hidemi Sato
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Eggs ◽  
Tubulin Paracrystals

The effects of mitotic inhibitors on the structure of vinblastine-induced tubulin paracrystals from sea-urchin eggs

1976 ◽  
Vol 20 (1) ◽  
pp. 91-100
Author(s):  
D. Starling
Keyword(s):  
Sea Urchin ◽  
Ultrastructural Level ◽  
Tubulin Polymerization ◽  
Sea Urchin Eggs ◽  
Mitotic Inhibitors ◽  
Vinblastine Sulphate ◽  
Tubulin Paracrystals ◽  
Careful Standardization

Vinblastine sulphate (VLB) is known to induce in vivo formation of tubulin paracrystals in sea-urchin eggs. Corresponding paracrystals have been prepared in the presence of both vinblastine sulphate and other mitoclasic agents. Careful standardization of conditions was required to restrict the formation of alternative forms of the paracrystals induced by vinblastine alone. Comparisons were made between preparations in terms of paracrystal shape, size, proportion of eggs containing paracrystals, number per egg and their relative times of first appearance. A correlation between such properties were established. Comparison of paracrystals at the ultrastructural level showed them all to be similar regardless of the drugs present during their formation. The implications of tubulin polymerization in the presence of mitoclasic agents are discussed and mechanisms for paracrystal enhancement by combinations of such drugs are suggested. Some similarities of paracrystal and microtubule seeding are discussed together with the activation of tubulin in the pool.

Two ultrastructurally distinct tubulin paracrystals induced in sea-urchin eggs by vinblastine sulphate

1976 ◽  
Vol 20 (1) ◽  
pp. 79-89 ◽  
Author(s):  
D. Starling
Keyword(s):  
Sea Urchin ◽  
Binding Sites ◽  
Mammalian Cells ◽  
The Other ◽  
Sea Urchin Eggs ◽  
Vincristine Sulphate ◽  
Vinblastine Sulphate ◽  
Available Information ◽  
Tubulin Paracrystals

Two types of ultrastructurally distinct tubulin paracrystals have been induced in sea-urchin eggs with vinblastine sulphate (VLB) under different sets of conditions. One type of paracrystal appears to consist of hexagonally-close packed microtubules and closely resembles paracrystals present in mammalian cells treated with vinblastine or vincristine sulphate, but not previously reported in sea-urchin eggs. The other type is also made up of tubulin subunits, but these do not seem to have polymerized into microtubules. Both types of paracrystal are induced in sea-urchin eggs in the presence of VLB at a time when tubulin subunits would not normally polymerize. Possible mechanisms for tubulin activation and the induction of paracrystal formation are discussed in respect to the available information on the binding sites of the tubulin subunits.

scholarly journals Identification of polysialic acid-containing glycoprotein in the jelly coat of sea urchin eggs. Occurrence of a novel type of polysialic acid structure.

1994 ◽  
Vol 269 (36) ◽  
pp. 22712-22718 ◽  
Author(s):  
S. Kitazume ◽  
K. Kitajima ◽  
S. Inoue ◽  
F.A. Troy ◽  
J.W. Cho ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Polysialic Acid ◽  
Sea Urchin Eggs ◽  
Jelly Coat ◽  
Acid Structure

scholarly journals A Novel Sialic Acid and Fucose-containing Disaccharide Isolated from the Jelly Coat of Sea Urchin Eggs

1973 ◽  
Vol 248 (2) ◽  
pp. 629-631
Author(s):  
Kyoko Hotta ◽  
Masaharu Kurokawa ◽  
Saburo Isaka
Keyword(s):  
Sialic Acid ◽  
Sea Urchin ◽  
Sea Urchin Eggs ◽  
Jelly Coat

scholarly journals Ovothiol: a novel thiohistidine compound from sea urchin eggs that confers NAD(P)H-O2 oxidoreductase activity on ovoperoxidase.

1986 ◽  
Vol 261 (28) ◽  
pp. 13056-13063
Author(s):  
E Turner ◽  
R Klevit ◽  
P B Hopkins ◽  
B M Shapiro
Keyword(s):  
Sea Urchin ◽  
Oxidoreductase Activity ◽  
Sea Urchin Eggs

scholarly journals Dynein isoforms in sea urchin eggs.

1988 ◽  
Vol 263 (14) ◽  
pp. 6759-6771 ◽  
Author(s):  
M E Porter ◽  
P M Grissom ◽  
J M Scholey ◽  
E D Salmon ◽  
J R McIntosh
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Eggs
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