Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming

2003 ◽  
pp. 73-76 ◽  
Author(s):  
Tim McCreery ◽  
Tom Helentjaris
Keyword(s):  
Dna Hybridization ◽  
Modified Nucleotides ◽  
Hybridization Probes

scholarly journals Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.

Genetics ◽  
1991 ◽  
Vol 127 (4) ◽  
pp. 681-698 ◽  
Author(s):  
A J Link ◽  
M V Olson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Physical Map ◽  
Single Copy ◽  
Restriction Endonucleases ◽  
Yeast Genome ◽  
Hybridization Probes ◽  
Copy Dna ◽  
Single Copy Dna

Abstract A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.

“Multicolor” Electrochemical Labeling of DNA Hybridization Probes with Osmium Tetroxide Complexes

2007 ◽  
Vol 79 (3) ◽  
pp. 1022-1029 ◽  
Author(s):  
Miroslav Fojta ◽  
Pavel Kostečka ◽  
Mojmír Trefulka ◽  
Luděk Havran ◽  
Emil Paleček
Keyword(s):  
Dna Hybridization ◽  
Osmium Tetroxide ◽  
Hybridization Probes

scholarly journals Identification of Borrelia burgdorferi and B. hermsii using DNA hybridization probes.

1989 ◽  
Vol 27 (8) ◽  
pp. 1734-1738 ◽  
Author(s):  
T G Schwan ◽  
W J Simpson ◽  
M E Schrumpf ◽  
R H Karstens
Keyword(s):  
Borrelia Burgdorferi ◽  
Dna Hybridization ◽  
Hybridization Probes
Sign in / Sign up

Export Citation Format

Share Document