This book is a counter-history of the emergence of lesbian sexuality in early cinema. Drawing on the critical insights of queer theory and the history of sexuality, it challenges approaches to lesbian representation, initially by reframing the emergence of lesbian figures in cinema in the late 1920s and early 1930s as only the most visible and belated signs of an array of strategies of sexuality. The emergence of lesbian representation and spectatorship in early cinema is not a linear progression and consolidation but rather arises across multiple sites in dispersed forms that are modern and backward-looking, recursive and anachronistic. In this tumultuous period, new but not always coherent sexual knowledges and categories emerge, even as older modalities of homoeroticism persist. The book articulates some of the discursive and institutional processes by which women’s same-sex desires and identities have been reorganized as impossible, marginal or—perhaps not so surprisingly—central to new forms of cinematic representation and spectatorship. Complicating the critical consensus of feminist film theory and history, the book foregrounds the centrality of women’s same-sex desire to historically distinct cinematic discourses of both homo- and heterosexuality. It articulates across its chapters the emergence of lesbian sexuality—and that of its intimate “other,” heterosexuality—as the effect of diverse discursive operations of early cinema, considered as a complex assemblage of film texts, exhibition practices, modes of female spectatorship, and reception.