scholarly journals Deciphering Dead-End Docking of Large Dense Core Vesicles in Bovine Chromaffin Cells

2013 ◽  
Vol 33 (43) ◽  
pp. 17123-17137 ◽  
Author(s):  
Sandra Hugo ◽  
Ekta Dembla ◽  
Mahantappa Halimani ◽  
Ulf Matti ◽  
Jens Rettig ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Dead End ◽  
Large Dense Core Vesicles ◽  
Bovine Chromaffin Cells

scholarly journals Trachynilysin mediates SNARE-dependent release of catecholamines from chromaffin cells via external and stored Ca2+

2000 ◽  
Vol 113 (7) ◽  
pp. 1119-1125 ◽  
Author(s):  
F.A. Meunier ◽  
C. Mattei ◽  
P. Chameau ◽  
G. Lawrence ◽  
C. Colasante ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Motor Nerve ◽  
Neuroendocrine Cells ◽  
Dense Core ◽  
Frog Neuromuscular Junction ◽  
Dense Core Vesicles ◽  
Protein Receptor ◽  
Large Dense Core Vesicles ◽  
Quantal Transmitter Release ◽  
Bovine Chromaffin Cells

Trachynilysin, a 159 kDa dimeric protein purified from stonefish (Synanceia trachynis) venom, dramatically increases spontaneous quantal transmitter release at the frog neuromuscular junction, depleting small clear synaptic vesicles, whilst not affecting large dense core vesicles. The basis of this insensitivity of large dense core vesicles exocytosis was examined using a fluorimetric assay to determine whether the toxin could elicit catecholamine release from bovine chromaffin cells. Unlike the case of the motor nerve endings, nanomolar concentrations of trachynilysin evoked sustained Soluble N-ethylmaleimide-sensitive fusion protein Attachment Protein REceptor-dependent exocytosis of large dense core vesicles, but only in the presence of extracellular Ca2+. However, this response to trachynilysin does not rely on Ca2+ influx through voltage-activated Ca2+ channels because the secretion was only slightly affected by blockers of L, N and P/Q types. Instead, trachynilysin elicited a localized increase in intracellular fluorescence monitored with fluo-3/AM, that precisely co-localized with the increase of fluorescence resulting from caffeine-induced release of Ca2+ from intracellular stores. Moreover, depletion of the latter stores inhibited trachynilysin-induced exocytosis. Thus, the observed requirement of external Ca2+ for stimulation of large dense core vesicles exocytosis from chromaffin cells implicates plasma membrane channels that signal efflux of Ca2+ from intracellular stores. This study also suggests that the bases of exocytosis of large dense core vesicles from motor nerve terminals and neuroendocrine cells are distinct.

The GTPase Rab3a is associated with large dense core vesicles in bovine chromaffin cells and rat PC12 cells

1995 ◽  
Vol 108 (4) ◽  
pp. 1639-1649 ◽  
Author(s):  
F. Darchen ◽  
J. Senyshyn ◽  
W.H. Brondyk ◽  
D.J. Taatjes ◽  
R.W. Holz ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Pc12 Cells ◽  
Immunoelectron Microscopy ◽  
Chromaffin Cells ◽  
Secretory Granules ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Large Dense Core Vesicles ◽  
Bovine Chromaffin Cells ◽  
Specific Association

Small GTPases of the rab family control intracellular vesicle traffic in eukaryotic cells. Although the molecular mechanisms underlying the activity of the Rab proteins have not been elucidated yet, it is known that the function of these proteins is dependent on their precise subcellular localization. It has been suggested that Rab3a, which is mainly expressed in neural and endocrine cells, might regulate exocytosis. Recently, direct experimental evidence supporting this hypothesis has been obtained. Consistent with such a role for Rab3a in regulated exocytosis was the previously reported specific association of Rab3a with synaptic vesicles and with secretory granules in adrenal chromaffin cells. Since the latter result, based on subcellular fractionation, has been controversial, we have re-investigated the subcellular localization of this GTP-binding protein by using a combination of morphological techniques. Bovine chromaffin cells were labelled with an affinity-purified polyclonal anti-Rab3a antibody and analyzed by confocal microcopy. Rab3a was found to colocalize partially with dopamine beta-hydroxylase, a chromaffin granule marker. In agreement with this observation, immunoelectron microscopy revealed a specific staining of chromaffin granules. In addition to large dense core vesicles, some small vesicles were labelled. To eliminate the possibility that the staining was due to a Rab3a-related protein, we investigated by immunoelectron microscopy the localization of an epitope-tagged Rab3a expressed in rat PC12 cells. Secretory granules were specifically labelled, whereas clear microvesicles were not. These results provide further evidence supporting a specific association of the GTPase Rab3a with large dense core secretory vesicles.

scholarly journals Munc13-1 acts as a priming factor for large dense-core vesicles in bovine chromaffin cells

2000 ◽  
Vol 19 (14) ◽  
pp. 3586-3596 ◽  
Author(s):  
Uri Ashery ◽  
Frederique Varoqueaux ◽  
Thomas Voets ◽  
Andrea Betz ◽  
Pratima Thakur ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Large Dense Core Vesicles ◽  
Bovine Chromaffin Cells

Vesicular Exocytosis under Hypotonic Conditions Shows Two Distinct Populations of Dense Core Vesicles in Bovine Chromaffin Cells

ChemPhysChem ◽  
2007 ◽  
Vol 8 (4) ◽  
pp. 578-585 ◽  
Author(s):  
Christian Amatore ◽  
Stéphane Arbault ◽  
Imelda Bonifas ◽  
Frédéric Lemaître ◽  
Yann Verchier
Keyword(s):  
Chromaffin Cells ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Bovine Chromaffin Cells ◽  
Vesicular Exocytosis

scholarly journals Impaired maturation of large dense-core vesicles in muted-deficient adrenal chromaffin cells

2015 ◽  
Vol 128 (7) ◽  
pp. 1365-1374 ◽  
Author(s):  
Z. Hao ◽  
L. Wei ◽  
Y. Feng ◽  
X. Chen ◽  
W. Du ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Dense Core ◽  
Adrenal Chromaffin Cells ◽  
Dense Core Vesicles ◽  
Large Dense Core Vesicles ◽  
Adrenal Chromaffin
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