1P035 Structure, Stability, and Function of Deletion Mutants atβC-βD Loop of Dihydrofolate Reductase from Escherichia coli

Seibutsu Butsuri ◽

10.2142/biophys.45.s40_3 ◽

2005 ◽

Vol 45 (supplement) ◽

pp. S40

Author(s):

Yuji Horiuchi ◽

Eiji Ohmae ◽

Kunihiko Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Structure Stability ◽

Deletion Mutants ◽

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Effects of Five-Tryptophan Mutations on Structure, Stability and Function of Escherichia coli Dihydrofolate Reductase

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a003004 ◽

2001 ◽

Vol 130 (3) ◽

pp. 439-447 ◽

Author(s):

E. Ohmae ◽

Y. Sasaki ◽

K. Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Structure Stability ◽

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Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1

Extremophiles ◽

10.1007/s00792-015-0732-7 ◽

2015 ◽

Vol 19 (2) ◽

pp. 479-493 ◽

Author(s):

Yurina Miyashita ◽

Eiji Ohmae ◽

Kaoru Nakasone ◽

Katsuo Katayanagi

Keyword(s):

Dihydrofolate Reductase ◽

Structure Stability ◽

Haloarcula Japonica ◽

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Effects of Point Mutations at the Flexible Loop Glycine-67 of Escherichia coli; Dihydrofolate Reductase on its Stability and Function

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a021299 ◽

1996 ◽

Vol 119 (4) ◽

pp. 703-710 ◽

Author(s):

E. Ohmae ◽

K. Iriyama ◽

S. Ichihara ◽

K. Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Point Mutations ◽

Flexible Loop ◽

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Effects of Point Mutations at the Flexible Loop Alanine-145 of Escherichia coli Dihydrofolate Reductase on Its Stability and Function

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a022013 ◽

1998 ◽

Vol 123 (5) ◽

pp. 839-846 ◽

Author(s):

E. Ohmae ◽

K. Ishimura ◽

M. Iwakura ◽

K. Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Point Mutations ◽

Flexible Loop ◽

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Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2009.12.011 ◽

2010 ◽

Vol 1804 (4) ◽

pp. 846-855 ◽

Author(s):

Yuji Horiuchi ◽

Eiji Ohmae ◽

Shin-ichi Tate ◽

Kunihiko Gekko

Keyword(s):

Escherichia Coli ◽

Enzymatic Activity ◽

Structural Stability ◽

Dihydrofolate Reductase ◽

Deletion Mutants ◽

Coupling Effects

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Effects of Mutation at Methionine-42 of Escherichia coli Dihydrofolate Reductase on Stability and Function: Implication of Hydrophobic Interactions

The Journal of Biochemistry ◽

10.1093/jb/mvi079 ◽

2005 ◽

Vol 137 (5) ◽

pp. 643-652 ◽

Author(s):

Eiji Ohmae ◽

Yukari Fukumizu ◽

Masahiro Iwakura ◽

Kunihiko Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Hydrophobic Interactions ◽

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Nonadditive Effects of Double Mutations at the Flexible Loops, Glycine-67 and Glycine-121, of Escherichia coli Dihydrofolate Reductase on Its Stability and Function

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a021913 ◽

1998 ◽

Vol 123 (1) ◽

pp. 33-41 ◽

Author(s):

E. Ohmae ◽

K. Iriyama ◽

S. Ichihara ◽

K. Gekko

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

And Function ◽

Nonadditive Effects ◽

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Faculty Opinions recommendation of Improving protein solubility: the use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1030480.359463 ◽

2006 ◽

Author(s):

David Waugh

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Protein Solubility ◽

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Faculty Opinions recommendation of Conformational change of the methionine 20 loop of Escherichia coli dihydrofolate reductase modulates pKa of the bound dihydrofolate.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1086898.539831 ◽

2007 ◽

Author(s):

Vivian Cody

Keyword(s):

Escherichia Coli ◽

Conformational Change ◽

Dihydrofolate Reductase

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Faculty Opinions recommendation of Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.719587029.793511938 ◽

2015 ◽

Author(s):

Amnon Kohen

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

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