scholarly journals Purification, properties and recognition sequence of site-specific restriction endonuclease from "Acetobacter liquefaciens".

1983 ◽  
Vol 29 (2) ◽  
pp. 157-166 ◽  
Author(s):  
YUZO YAMADA ◽  
HAJIME YOSHIOKA ◽  
JOJI SASAKI ◽  
YASUTAKA TAHARA
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Sequence ◽  
Site Specific ◽  
Specific Restriction

Purification, Properties and Recognition Sequence of Site-specific Restriction Endonuclease from “Acetobacter xylinus”

1983 ◽  
Vol 47 (12) ◽  
pp. 2871-2879
Author(s):  
Hajime Yoshioka ◽  
Hiroshi Nakamura ◽  
Joji Sasaki ◽  
Yasutaka Tahara ◽  
Yuzo Yamada
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Sequence ◽  
Site Specific ◽  
Specific Restriction

scholarly journals INHERITANCE OF THE 2μm DNA PLASMID FROM SACCHAROMYCES

Genetics ◽  
1977 ◽  
Vol 86 (1) ◽  
pp. 73-84
Author(s):  
Dennis M Livingston
Keyword(s):  
Restriction Endonuclease ◽  
Vegetative Growth ◽  
Restriction Site ◽  
Site Specific ◽  
Restriction Sites ◽  
Different Types ◽  
Ecori Restriction ◽  
Specific Restriction ◽  
Dna Plasmid ◽  
Restriction Endonuclease Recognition Site

ABSTRACT A variety of Saccharomyces strains were examined for the presence of 2µ DNA and, if present, for the pattern of fragments produced by its digestion with site-specific (restriction) endonucleases. Two strains were found that did not contain detectable levels of 2µ DNA, and two strains contained 2µ DNA molecules having only one EcoRI restriction endonuclease recognition site rather than the usual two.—A haploid containing 2µ DNA with one EcoRI restriction site was mated with a haploid containing 2µ DNA with two EcoRI restriction sites and the resulting diploid maintained both types during vegetative growth. Sporulation of the diploid produced four spores, and the clones from these spores contained both types.—A haploid lacking 2µ DNA was mated with a haploid containing 2µ DNA and the resulting diploid contained 2µ DNA. The four clones derived from the haploid spores after sporulation of this diploid all contained 2µ DNA. A rho  - strain without 2µ DNA was mated to a rho  + strain with 2µ DNA, and heteroplasmons were selected that had received the nucleus from the strain without 2µ DNA and the mitochondria from the strain with 2µ DNA. Twelve of twenty-four such clones contained 2µ DNA.—I conclude that: (1) the different types of 2µ DNA identified in these strains do not restrict one another, (2) the different types are inherited extrachromosomally, (3) lack of 2µ DNA in two strains is not due to the absence of genes needed for maintenance and (4) the approximately 100 copies of 2µ DNA contained within a single cell are probably clustered within one or a few cytoplasmic organelles.

scholarly journals Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens.

1981 ◽  
Vol 145 (2) ◽  
pp. 873-877 ◽  
Author(s):  
J Timko ◽  
A H Horwitz ◽  
J Zelinka ◽  
G Wilcox
Keyword(s):  
Restriction Endonuclease ◽  
Site Specific ◽  
Streptomyces Aureofaciens ◽  
Specific Restriction ◽  
A Site

scholarly journals Site-specific restriction endonuclease from the filamentous cyanobacteriumNostocsp. MAC PCC 8009

FEBS Letters ◽  
1985 ◽  
Vol 179 (1) ◽  
pp. 129-132 ◽  
Author(s):  
Reginald H. Lau ◽  
Louis P. Visentin ◽  
Stan M. Martin ◽  
Jason D. Hofman ◽  
W.Ford Doolittle
Keyword(s):  
Restriction Endonuclease ◽  
Site Specific ◽  
Specific Restriction
Sign in / Sign up

Export Citation Format

Share Document