Constrained Peptides Mimic a Viral Suppressor of RNA Silencing
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The structure-based design of constrained alpha-helical peptides derived from the viral suppressor of RNA silencing TAV2b is described. We observe that the introduction of two inter-side chain crosslinks provides peptides with increased alpha-helicity and protease stability. One of these modified peptides (B3) shows high affinity for different double-stranded RNA structures including a palindromic siRNA as well as microRNA-21 and its precursor pre-miR-21. Notably, B3 binding to pre-miR-21 inhibits Dicer processing in a biochemical assay. As a further characteristic this peptide also exhibits cellular entry. <br>
2020 ◽
2011 ◽
Vol 412
(2)
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pp. 165-172
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2002 ◽
Vol 99
(23)
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pp. 15228-15233
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2014 ◽
Vol 290
(5)
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pp. 3106-3120
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