Effect of Pre-treatment of Zeatin on Multiple Shoot Regeneration from Leaf Explants of Aquatic Plant Bacopa monnieri (L.) Wettst.

Investigation on in vitro multiple shoot regeneration in Bacopa monnieri (L.) Penn. using leaf and nodal explants was carried out on MS containing various concentrations and combinations of BAP, Kn, NAA and 2,4-D. Of the two explants, leaf showed the best response towards shoot regeneration and subsequent plant development on MS with 1.0 mg/l BAP and 0.25 mg/l Kn. In this combination, the mean number of shoots/explant was 10.6 ± 0.11 in leaf and 9.6 ± 0.29 in nodal explants. Maximum shoot length was recorded as 12.6 ± 0.21 and 11.20 ± 0.30 from leaf and nodal explants after six weeks of culture, respectively. Half strength of MS supplemented with 0.25 mg/l IBA was found to be the best medium for root formation. The in vitro regenerated plantlets were successfully transplanted in soil after acclimatization. Plant Tissue Cult. & Biotech. 30(1): 57-63, 2020 (June)

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Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell

Plant Cell Reports ◽

10.1007/s002990050684 ◽

1999 ◽

Vol 18 (11) ◽

pp. 919-923 ◽

Cited By ~ 29

Author(s):

N. Shrivastava ◽

M. Rajani

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Multiple Shoot ◽

Bacopa Monnieri ◽

Culture Studies ◽

Multiple Shoot Regeneration

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TDz Induced Multiple Shoot Regeneration and in Vitro Flowering From Nodal Explant in Withania Somnifera (L.)

International Journal of Scientific Research ◽

10.15373/22778179/apr2014/20 ◽

2012 ◽

Vol 3 (4) ◽

pp. 50-52

Author(s):

Nishesh Sharma ◽

◽

Manjul Dhiman ◽

Eapen P Koshy

Keyword(s):

Shoot Regeneration ◽

Withania Somnifera ◽

Multiple Shoot ◽

In Vitro Flowering ◽

Nodal Explant ◽

Multiple Shoot Regeneration

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An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v18i1.3246 ◽

2009 ◽

Vol 18 (1) ◽

pp. 17-24

Author(s):

Md. Nesawar Miah ◽

Shahina Islam ◽

Syed Hadiuzzaman

Keyword(s):

Shoot Regeneration ◽

Multiple Shoots ◽

Multiple Shoot ◽

Nodal Explants ◽

Cow Dung ◽

Half Strength ◽

Multiple Shoot Regeneration ◽

Shoot Tip Explants ◽

In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

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An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

Natural Product Communications ◽

10.1177/1934578x1000501223 ◽

2010 ◽

Vol 5 (12) ◽

pp. 1934578X1000501

Author(s):

Sanjog T. Thul ◽

Arun K. Kukreja

Keyword(s):

Shoot Regeneration ◽

Multiple Shoots ◽

Shoot Elongation ◽

Shoot Formation ◽

Multiple Shoot ◽

Ms Medium ◽

Mentha X Piperita ◽

Half Strength ◽

Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

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