Isolating some fungi strains from diease roots of Dipterocarpus dyeri planted at the nursery in Dong Nai province

Thu Dau Mot University Journal of Science ◽

10.37550/tdmu.ejs/2020.02.046 ◽

2020 ◽

pp. 187-194

Keyword(s):

Aspergillus Nidulans ◽

28S Rdna ◽

Timber Industry ◽

Urban Streets ◽

Rdna Sequencing ◽

Moist Forest

Dipterocarpus dyeri is a typical plant of tropical evergreen moist forest at Southeast Vietnam. These plants have been planted popularly at parks and urban streets for the shade and it has been commonly materials for timber industry. Multiplication of Dipterocarpus dyeri at nurseries could face to some diseases, such as the withered disease cause serial death. Our study isolated three disease fungi strains from the root areas of the diseased Dipterocarpus dyeri planted Ma Da nursery, Dong Nai province. Result of 28s rDNA sequencing showed these fungi belong to Ophiostoma eucalypticagena, Aspergillus nidulans and Collectotrichum gloeosporioides. This result is base for conducting the following studies to control the withered disease on Dipterocarpus dyeri at the nursery.

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Hyperparasitism on Plasmopara viticola by Simplicillium lanosoniveum

PhytoFrontiers™ ◽

10.1094/phytofr-09-21-0061-sc ◽

2021 ◽

Author(s):

Hongmiao Shen ◽

Xiaomeng Shi ◽

Longxian Ran

Keyword(s):

Downy Mildew ◽

Potential Application ◽

Plasmopara Viticola ◽

Fungal Strain ◽

28S Rdna ◽

Vitis Labrusca ◽

First Report ◽

Rdna Sequencing ◽

Grape Downy Mildew ◽

Causal Pathogen

A previously unknown association between Plasmopara viticola, the causal pathogen of grape downy mildew, and the mycoparasite strain F2 was described in this paper. F2 could be consistently isolated from the mildew of diseased spots on grape leaves of Kyoho (Vitis labrusca × V. vinifera cv. Kyoho) infected with P. viticola, but neither from the surfaces of healthy grape leaves nor within lesions of other diseases. It could be observed that strain F2 was capable of coiling around sporangiophores and sporangia of P. viticola under microscope, causing hyphae deformation, decreasing the pathogen growth, and reducing the number of sporangia. This fungal strain was identified as Simplicillium lanosoniveum on the basis of morphological characterizations and 28S rDNA sequencing. To our knowledge, this is the first report of S. lanosoniveum with hyperparasitic characteristics on P. viticola, and the hyperparasite has potential application in the control of grape downy mildew.

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Real-Time PCR and Sequencing Assays for Rapid Detection and Identification of Avian Schistosomes in Environmental Samples

Applied and Environmental Microbiology ◽

10.1128/aem.00750-15 ◽

2015 ◽

Vol 81 (12) ◽

pp. 4207-4215 ◽

Cited By ~ 7

Author(s):

Narayanan Jothikumar ◽

Bonnie J. Mull ◽

Sara V. Brant ◽

Eric S. Loker ◽

Jeremy Collinson ◽

...

Keyword(s):

Lake Water ◽

Water Samples ◽

18S Rdna ◽

28S Rdna ◽

Taqman Assay ◽

Rrna Gene ◽

Content Type ◽

Cercarial Dermatitis ◽

Rdna Sequencing ◽

Avian Schistosomes

ABSTRACTCercarial dermatitis, also known as swimmer's itch, is an allergenic skin reaction followed by intense itching caused by schistosome cercariae penetrating human skin. Cercarial dermatitis outbreaks occur globally and are frequently associated with freshwater lakes and are occasionally associated with marine or estuarine waters where birds reside year-round or where migratory birds reside. In this study, a broadly reactive TaqMan assay targeting 18S rRNA gene (ribosomal DNA [rDNA]) sequences that was based on a genetically diverse panel of schistosome isolates representing 13 genera and 20 species (the 18S rDNA TaqMan assay) was developed. A PCR assay was also developed to amplify a 28S rDNA region for subsequent sequencing to identify schistosomes. When applied to surface water samples seeded withSchistosoma mansonicercariae, the 18S rDNA TaqMan assay enabled detection at a level of 5S. mansonicercariae in 100 liters of lake water. The 18S rDNA TaqMan and 28S rDNA PCR sequencing assays were also applied to 100-liter water samples collected from lakes in Nebraska and Wisconsin where there were reported dermatitis outbreaks. Avian schistosome DNA was detected in 11 of 34 lake water samples using the TaqMan assay. Further 28S rDNA sequence analysis of positive samples confirmed the presence of avian schistosome DNA and provided a preliminary identification of the avian schistosomes in 10 of the 11 samples. These data indicate that the broadly schistosome-reactive TaqMan assay can be effective for rapid screening of large-volume water samples for detection of avian schistosomes, thereby facilitating timely response actions to mitigate or prevent dermatitis outbreaks. Additionally, samples positive by the 18S rDNA TaqMan assay can be further assayed using the 28S rDNA sequencing assay to both confirm the presence of schistosomes and contribute to their identification.

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