tryptophan quenching
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2014 ◽  
Vol 77 (7) ◽  
pp. 510-516 ◽  
Author(s):  
Siddharth Nanguneri ◽  
Benjamin Flottmann ◽  
Frank Herrmannsdörfer ◽  
Kuner Thomas ◽  
Mike Heilemann

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Mary E. Peek ◽  
Abhinav Bhatnagar ◽  
Nael A. McCarty ◽  
Susu M. Zughaier

Pseudomonas aeruginosais the most common pathogen that persists in the cystic fibrosis lungs. Bacteria such asP. aeruginosasecrete siderophores (iron-chelating molecules) and the host limits bacterial growth by producing neutrophil-gelatinase-associated lipocalin (NGAL) that specifically scavenges bacterial siderophores, therefore preventing bacteria from establishing infection.P. aeruginosaproduces a major siderophore known as pyoverdine, found to be important for bacterial virulence and biofilm development. We report that pyoverdine did not bind to NGAL, as measured by tryptophan fluorescence quenching, while enterobactin bound to NGAL effectively causing a strong response. The experimental data indicate that pyoverdine evades NGAL recognition. We then employed a molecular modeling approach to simulate the binding of pyoverdine to human NGAL using NGAL’s published crystal structures. The docking of pyoverdine to NGAL predicted nine different docking positions; however, neither apo- nor ferric forms of pyoverdine docked into the ligand-binding site in the calyx of NGAL where siderophores are known to bind. The molecular modeling results offer structural support that pyoverdine does not bind to NGAL, confirming the results obtained in the tryptophan quenching assay. The data suggest that pyoverdine is a stealth siderophore that evades NGAL recognition allowingP. aeruginosato establish chronic infections in CF lungs.


2009 ◽  
Vol 113 (27) ◽  
pp. 7981-7981 ◽  
Author(s):  
Denisio M. Togashi ◽  
Boguslaw Szczupak ◽  
Alan G. Ryder ◽  
Amandine Calvet ◽  
Muireann O’Loughlin

2009 ◽  
Vol 113 (12) ◽  
pp. 2757-2767 ◽  
Author(s):  
Denisio M. Togashi ◽  
Boguslaw Szczupak ◽  
Alan G. Ryder ◽  
Amandine Calvet ◽  
Muireann O’Loughlin

2008 ◽  
Vol 1780 (10) ◽  
pp. 1143-1147 ◽  
Author(s):  
Wolfgang Erker ◽  
Rüdiger Hübler ◽  
Heinz Decker

1998 ◽  
Vol 67 (4) ◽  
pp. 371-377 ◽  
Author(s):  
Marta E. Daraio ◽  
Axel Völker ◽  
Pedro F. Aramendia ◽  
Enrique San Román

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