Germination and initial development of Simira gardneriana seedling under water stress and at different temperatures

ABSTRACT The germination of seeds is affected by many factors, including water availability and temperature. This study aimed to evaluate the effects of water stress and different temperatures on germination and initial development of S. gardneriana M. R. Barbosa & Peixoto seedlings. The experiment was carried out in a completely randomized design, in a 7 x 4 factorial scheme, with seven potentials (0; -0.1; -0.2; -0.3; -0.4; -0.5 and - 0.6 MPa) and four temperatures (25, 30, 35 and 20-30 °C), with four replicates of 25 seeds. The analyzed variables were germination, percentage of normal seedlings, germination speed index, seedling length (shoot and root) and dry weight (shoot and root). There is a decrease in the germination and vigor of the seeds as the water potential decreases, being less affected under the temperature of 25 °C and very accelerated at 35 °C. The tolerance of S. gardneriana seed germination to water stress is variable between the temperatures and limiting to the formation of normal seedlings from the potential -0.5 MPa at temperatures of 25 to 30 °C and -0.4 and -0.2 MPa for 20-30 and 35 °C, respectively.

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Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

Acta Scientiarum Biological Sciences ◽

10.4025/actascibiolsci.v41i1.46030 ◽

2019 ◽

Vol 41 ◽

pp. e46030

Author(s):

João Everthon da Silva Ribeiro ◽

Arliston Pereira Leite ◽

Jackson Silva Nóbrega ◽

Edna Ursulino Alves ◽

Riselane de Lucena Alcântara Bruno ◽

...

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Seed Vigor ◽

Randomized Design ◽

Temperature Regimes ◽

Seedling Length ◽

Physiological Quality ◽

Mean Germination Time ◽

Completely Randomized Design ◽

Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

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Physiological potential of Dyckia spp. bromeliad seeds under different temperatures

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n5p2639 ◽

2021 ◽

Vol 42 (5) ◽

pp. 2639-2650

Author(s):

Verônica Pellizzaro Moresco ◽

◽

Mônica Satie Omura ◽

Jean Carlo Baudraz de Paula ◽

Felipe Favoretto Furlan ◽

...

Keyword(s):

Growth And Development ◽

Ornamental Plants ◽

Dry Weight ◽

Natural Environments ◽

The Third ◽

Tukey Test ◽

Seed Physiology ◽

Randomized Design ◽

Different Temperatures ◽

Completely Randomized Design

Bromeliads have been gaining ground in the economic scenario due mainly to their use as ornamental plants. Bromeliads of the genus Dyckia exhibit different morphologies, and therefore, have relevance in this market. However, some species are threatened due to the extraction of plants from their natural environments. Many of these species are endemic and native to Brazil and there are few studies on their seed physiology; hence, expansion of research into this topic is essential. An alternative for the preservation of such plants is the production of seedlings using seeds. To this end, the objective of this work was to evaluate the physiological potential of seeds of Dyckia brevifolia, Dyckia beateae, and Dyckia excelsa at different temperatures, and determine the number of days to carry out the tests. A completely randomized design was adopted, with three species of Dyckia, analyzed separately, and four temperatures (20, 25, 30, and 35 °C). The following were evaluated: first germination count, germination, germination speed index, length of seedling, and seedling dry weight. The data were analyzed using the Sisvar software for normality and homogeneity, and when the assumptions were met, they were subjected to analysis of variance using the F test (p < 0.05) and the means were compared using the Tukey test at 5% probability. Seeds of D. excelsa emit the radicle more quickly at 20 and 35 °C; however, temperatures of 25 and 30 °C provide better conditions for seedling growth and development. The greatest vigor of D. brevifolia seeds is observed at temperatures of 30 and 35 °C. Seeds of D. beateae emit the radicle more quickly at 20 °C, and the largest seedlings are observed at 35 °C. The first germination count of D. brevifolia, D. beateae, and D. excelsa should be evaluated on the third day after sowing. The germination evaluations for D. excelsa and D. beateae should be carried out on the ninth day after sowing, and those for D. brevifolia on the sixth day after sowing.

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Physiological quality of parsley seeds in function of size

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n3supl1p1499 ◽

2021 ◽

Vol 42 (3Supl1) ◽

pp. 1499-1510

Author(s):

Renato Téo de Barros ◽

◽

Cibele Chalita Martins ◽

Tatiane Sanches Jeromini ◽

Givanildo Zildo da Silva ◽

...

Keyword(s):

Seed Size ◽

Dry Weight ◽

Germination Percentage ◽

Randomized Design ◽

Physiological Quality ◽

Completely Randomized Design ◽

Germination Vigor ◽

Selection Of

Parsley seeds are commercialized without classification by size during processing, unlike other crops. This classification procedure allows the standardization of seed size for high precision in sowing, disposal of unwanted material, and selection of high quality seeds. The aim of this study was to verify the effect of seed size on germination, vigor, and yield performance of two parsley cultivars. Seeds from eight lots of parsley of ‘Portuguesa’ and ‘Chacareira’ cultivar were separated into three size classes, represented by the seeds retained in the sieves of 0.84, 1.00 and 1.41mm mesh opening. After classification, seeds were evaluated in the laboratory for water content, germination percentage, first count, and thousand seed weight. In the field, the fresh and dry weight of aerial part and the final number of plants (stand) were evaluated after 78 days of sowing. The experimental design was the completely randomized design in an 8 x 3 factorial scheme (eight lots and three sieve opening) with four replicates, for both cultivars. Data obtained were subjected to analysis of variance and means were compared using Tukey test at 5% probability level. It was concluded that the parsley seeds separated by the 1.41 mm sieves showed higher vigor, and consequently, greater success in stand establishment at the field than those separated by other sieve size. The use of sieves in the classification of parsley seeds helps in obtaining lots with superior physiological quality.

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Pengaruh Berbagai Konsentrasi Atonik Terhadap Pertumbuhan Stek Panili (Vanilla plani

Agrotek ◽

10.30862/agt.v2i4.508 ◽

2018 ◽

Vol 2 (4) ◽

Author(s):

Amelia S. Sarungallo ◽

Bagyono Bagyono ◽

Aris Patiung ◽

Aris Patiung

Keyword(s):

Root Length ◽

Dry Weight ◽

Germination Percentage ◽

Single Node ◽

Growing Media ◽

Root Dry Weight ◽

Randomized Design ◽

Two Factors ◽

Media Types ◽

Completely Randomized Design

This research was aimed to identify the influence of Atonic cencentration to the growth of single-node Vanilla cutting planted at various growing media types. Completely randomized design was used in this research with two factors namely Atonic concentration (500 ppm, 1000 ppm, 1500 ppm, 2000 ppm, dan 2500 ppm) and growing media types (tanah, pasir, tanah + pupuk kandang, tanah + pasir, tanah + pasir + pupuk kandang). The result showed that there were no interaction between Atonic concentration and growing media types to the growth of single-node vanilla cutting. Furthermore, the application of Atonic concentration at 2000 ppm established the best result of root length (12,32 cm) and root dry weight (0,16); while the best result of germination percentage (92,23%) revealed by Atonic concentration of 1500 ppm. Regression analysis of germination percentage showed that the optimal concentration of Atonic was 1842 ppm with R<sup>2</sup> = 60,3%. Mixed media of soil + sand + dung performed the best result in root length, root dry weight and germination percentage.

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Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11919 ◽

2015 ◽

Vol 9 (1) ◽

pp. 6-11

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Seedling Length ◽

Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

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Morphometry and Germination of Passovia pyrifolia, Struthanthus marginatus and Phoradendron mucronatum Diaspores

Planta Daninha ◽

10.1590/s0100-83582018360100010 ◽

2018 ◽

Vol 36 (0) ◽

Author(s):

J.M.L. AZEVEDO ◽

C.G.G. SANTOS ◽

C.S. CAIRES ◽

J.C. ARAÚJO NETO ◽

R.C. SOUZA

Keyword(s):

Moisture Content ◽

Germination Rate ◽

Germination Percentage ◽

Percentage Germination ◽

Essential Characteristic ◽

Germination Time ◽

Randomized Design ◽

Different Temperatures ◽

Mean Germination Time ◽

Completely Randomized Design

ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.

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Influence of Shade and Bed Types on Attaining Optimum Temperature for The Germination of Peach (Prunus Persica L. Batsch) Seeds at Holetta, Central Ethiopia

Agricultural Science ◽

10.30560/as.v2n1p154 ◽

2020 ◽

Vol 2 (1) ◽

pp. p154

Author(s):

Habtam Setu ◽

Tajebe Mosie ◽

Kidist Firdie ◽

Tenagne Eshete ◽

Getaneh Sileshi

Keyword(s):

Prunus Persica ◽

Agricultural Research ◽

Germination Percentage ◽

Limiting Factor ◽

Optimum Temperature ◽

Randomized Design ◽

Germination Speed ◽

Completely Randomized Design ◽

Peach Trees ◽

Good Germination

Poor germination of peach seeds is the major limiting factor for rootstock production under the Holetta condition because of the seed dormancy problem. Stratification plays an important role in shortening the dormancy and increasing the germination of peach seeds. The study aimed to find easy stratification techniques for the germination of peach seeds using shade and different bed types. The experiment was carried out at the Holetta Agricultural Research Center in two successive years of 2017 and 2018. We got seeds used for this study from peach trees, which were well adapted for the Holetta area. It was arranged in a completely randomized design with five treatments of under tree shade on a flatbed, under tree shade in sunken bed, out of tree shade on a flatbed, out of tree shade in sunken bed and refrigerator as a standard check. They put all treatments except the refrigerator under the grass shade constructed 1 m3 wide. The treatments replicated four times, and each replication contained 200 seeds. The results showed that temperature is the most important factor affecting the germination percentage of the peach seeds. Seeds sown under the temperature close to 8 0C which is in the refrigerator found to have better germination percentage (47.1%) followed by the seeds sown in the grass shade under the tree shade both on sunken (35.8%) and flatbeds (34.9%). Peach seeds stratified in the refrigerator had good germination percentage whereas survival of the seedling was better at out of tree shade in sunken bed and out of tree shade on the flatbed. Even though the germination percentage was lower and the germination speed index was higher than the refrigerator. Seeds stratified at a temperature of above 17 OC experienced a poor germination percentage.

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MAGNETIC FIELD AND GIBBERELIC ACID AS PRE-GERMINATION TREATMENTS OF PASSION FRUIT SEEDS

Revista Ciência Agrícola ◽

10.28998/rca.v17i1.6522 ◽

2019 ◽

Vol 17 (1) ◽

pp. 15 ◽

Cited By ~ 2

Author(s):

Renata Diane Menegatti ◽

Luana Oliveira de Oliveira ◽

Ádrya Vanessa Lira da Costa ◽

Eugenia Jacira Bolacel Braga ◽

Valmor João Bianchi

Keyword(s):

Magnetic Field ◽

Dry Weight ◽

Germination Percentage ◽

Passion Fruit ◽

Passiflora Edulis ◽

Chemical Substances ◽

Randomized Design ◽

Gibberelic Acid ◽

Mean Germination Time ◽

Completely Randomized Design

<p>The aim of this study was to evaluate the effect of pre-germination treatments (magneto-priming and immersion of seeds in gibberellic acid solution) on variables associated with germination, emergence and vigor of Passiflora edulis seeds ‘BRS Gigante Amarelo’ cultivar. Seeds were extracted from fruits, washed, immersed for 6 hours in solutions with different GA3 concentrations and later arranged in a circular form in Petri dishes at temperature of 25°C, with and without exposure to magnetic field. Subsequently, analyses associated with the germination and emergency test were carried out. The experimental design was completely randomized design, with 3x2 factorial, three GA3 concentrations (0, 50 and 100 mg L-1) and presence/absence of magnetic field (MF), with four replicates of 20 seeds each. Variables germination percentage, germination speed index, mean germination time, percentage of emerged seedlings, emergence speed index, shoot length and root length and seedling dry weight were evaluated. Results indicate that the exposure of passion fruit seeds to MF in an isolated way stimulates seed germination, emergence and vigor, being an alternative to conventional treatments based on chemical substances.</p>

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Effect of Drying Temperature in the Physiological Quality of Jatropha curcas Seeds

Journal of Agricultural Science ◽

10.5539/jas.v10n9p443 ◽

2018 ◽

Vol 10 (9) ◽

pp. 443

Author(s):

Alessandra Olmo Dardengo ◽

Henrique Duarte Vieira ◽

Bruno Borges Deminicis ◽

Pedro Amorim Berbert ◽

Márcia Terezinha Ramos de Oliveira ◽

...

Keyword(s):

Global Warming ◽

Jatropha Curcas ◽

Fossil Fuels ◽

Germination Percentage ◽

Randomized Design ◽

Different Temperatures ◽

Physiological Quality ◽

Completely Randomized Design ◽

Jatropha Seeds

Stimulating the use of renewable energy instead of fossil fuels, especially biofuels, has become one of the alternatives to the issue of global warming. Of these species listed for the production of biodiesel is the Jatropha curcas crop. The aim of this study was to analyze the drying of the seeds of Jatropha, as well as to verify the effect of the different temperatures on the quality of the product, germination percentage and Emergence speed index (ESI). The experimental design was a completely randomized design, with four replications. Experimental drier was used to test the temperatures of 29, 35, 40, 45 and 50 °C and the data were compared by regression equation. The vigor and germination of the jatropha seeds were little affected as the drying temperatures were increased to 50 °C.

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Variation of beneficial effects of Trichoderma harzianum by the maize variety under water stress

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.051028 ◽

2018 ◽

Vol 5 (10) ◽

pp. 529-535

Author(s):

J. Chepsergon ◽

L. A. Mwamburi ◽

K. E. Kiprop

Keyword(s):

Water Stress ◽

Seedling Growth ◽

Trichoderma Harzianum ◽

Growth Parameters ◽

Morphological Characteristics ◽

Dry Weight ◽

Beneficial Effects ◽

Randomized Design ◽

Seedling Length ◽

Maize Variety

The aim of the study was to out whether the beneficial effect of T. harzianum in enhancing maize seed germination and seedling growth under water stress is dependent on the maize variety soil samples were collected from Marigat Rangeland, Kenya. Trichoderma harzianum was isolated using macro and micro-morphological characteristics. The present study employed a three-factor factorial (3 x 2 x 2) design, arranged in a completely randomized design (CRD) with three replications. There was no significant (p > 0.05) difference in percentage germination, seedling length, fresh weight, shoot-root dry weight, superoxide dismutase and catalase activities among the three varieties of maize. In addition, significant differences in seed germinations, seedling growth parameters and antioxidant enzyme enzymes were recorded between treated and control plants among the three varieties of maize. Maize variety had no significant influence on the beneficial activity of T. harzianum.

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