scholarly journals GENERATION OF REACTIVE OXYGEN SPECIES IN RAT EPIDIDYMAL SPERMATOZOA AFTER CYCLOPHOSPHAMIDE TREATMENT

2018 ◽  
Vol 11 (4) ◽  
pp. 437
Author(s):  
Kashmiri Zn ◽  
Sastry Ms
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Morphology ◽  
Reactive Oxygen ◽  
Wistar Rat ◽  
Sperm Function ◽  
Oxygen Species ◽  
Cyclophosphamide Treatment ◽  
Epididymal Spermatozoa ◽  
Vehicle Treated Control ◽  
Different Doses

 Objective: The aim of this study was to examine the effect of an anticancer drug cyclophosphamide (CPA) on generation of reactive oxygen species (ROS) in epididymal spermatozoa of Wistar rat, Rattus norvegicus. Methods: For this purpose, the rats were injected intraperitoneally with different doses of CPA (5 mg, 15 mg, and 20 mg/Kg BW for 2 weeks).Results: Treatment resulted into significantly increased level of ROS in CPA-treated groups when compared to the vehicle-treated control group.Conclusion: The results revealed that CPA has deleterious effect on the sperm morphology and physiology, which is dose and duration dependent and at certain doses cause the production of a number of reactive molecules and free radicals derived from molecular oxygen consequently resulting into adverse effect on the sperm function and hence on reproduction.

scholarly journals Analysis of the Relationship Between Defective Sperm Function and the Generation of Reactive Oxygen Species in Cases of Oligozoospermia

1989 ◽  
Vol 10 (3) ◽  
pp. 214-220 ◽  
Author(s):  
R. JOHN AITKEN ◽  
JANE S. CLARKSON ◽  
TIMOTHY B. HARGREAVE ◽  
D. STEWART IRVINE ◽  
FREDERICK C. W. WU
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Sperm Function ◽  
Oxygen Species ◽  
The Relationship

Reactive Oxygen Species (ROS)-Responsive Nanoprobe For Bioimaging And Targeted Therapy of Osteoarthritis

2021 ◽  
Author(s):  
Chong Shen ◽  
Ming Gao ◽  
Haimin Chen ◽  
Yanting Zhan ◽  
Qiumei Lan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cartilage Damage ◽  
Reactive Oxygen ◽  
Disease Classification ◽  
Oxygen Species ◽  
Stimulus Responsive ◽  
Targeting Peptide ◽  
Physiological Markers ◽  
Different Doses ◽  
Selection Of

Abstract Stimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is one of the leading causes of OA, we selected ROS as markers and designed a cartilage-targeting and ROS-responsive theranostic nanoprobe that is highly specific for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKC, which was then encapsulated with Dexamethasone (DEX) to form TKC@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKC@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics.

scholarly journals Spermatic mitochondria: role in oxidative homeostasis, sperm function and possible tools for their assessment

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 251-260 ◽  
Author(s):  
João Diego de Agostini Losano ◽  
Daniel de Souza Ramos Angrimani ◽  
Roberta Ferreira Leite ◽  
Bárbara do Carmo Simões da Silva ◽  
Valquíria Hyppolito Barnabe ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mitochondrial Function ◽  
Reactive Oxygen ◽  
Sperm Function ◽  
Oxygen Species ◽  
Functional Dynamics ◽  
Fertilization Capacity ◽  
Oxidative Homeostasis

SummaryDespite sperm mitochondrial relevance to the fertilization capacity, the processes involved in the production of ATP and functional dynamics of sperm mitochondria are not fully understood. One of these processes is the paradox involved between function and formation of reactive oxygen species performed by the organelle. Therefore, this review aimed to provide data on the role of sperm mitochondria in oxidative homeostasis and functionality as well the tools to assess sperm mitochondrial function.

Free radicals, lipid peroxidation and sperm function

1995 ◽  
Vol 7 (4) ◽  
pp. 659 ◽  
Author(s):  
RJ Aitken
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Reactive Oxygen ◽  
Human Spermatozoa ◽  
Sperm Function ◽  
Oxygen Species ◽  
Normal Sperm ◽  
Oxidative Processes ◽  
Mammalian Spermatozoa

The cellular generation of reactive oxygen species was first observed in mammalian spermatozoa in the late 1940s. The field then remained dormant for 30 years until Thaddeus Mann and Roy Jones published a series of landmark papers in the 1970s in which the importance of lipid peroxidation as a mechanism for damaging mammalian spermatozoa was first intimated. The subsequent demonstration that human spermatozoa produce reactive oxygen species and are susceptible to peroxidative damage has triggered intense interest in the role of oxidative stress in the aetiology of male infertility. Moreover, data have recently been obtained to indicate that, although excessive exposure to reactive oxygen species may be harmful to spermatozoa, in physiological amounts these molecules are of importance in the control of normal sperm function. This review considers the dualistic role of reactive oxygen species and sets out the current understanding of the importance of oxidative processes in both the physiology and the pathology of the human spermatozoon. Extra keywords: human spermatozoa, reactive oxygen species.

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