Correlation analysis of the parameters of oats assimilation apparatus and productivity elements

On sod-podzolic soils, new data is obtained on the functioning of the photosynthetic structures of oat leaves in various climatic conditions and of biomass accumulation (plant productivity). Based on three-year data (2015-2017) of field experiments with 29 oat genotypes, the relationship between the development of productivity elements and the characteristics of the plant assimilation apparatus is analyzed. The pigment content (chlorophyll a, chlorophyll b, carotenoids) was evaluated in acetone extracts on a UVmini-1240 spectrophotometer (SHIMADZU Corporation, Japan). Statistically significant correlations were established between the total leaf surface area and grain mass per plant (r = 0.664), the number of spikelets in a panicle, grain mass per panicle and the mass of the panicle itself (r = 0.548 ... 0.613). These correlations decreased during a drought and increased in conditions of excessive moisture. Oat genotypes with a larger sub-flag leaf had a larger panicle, with a large number of spikelets, grains and of larger grain mass per panicle and plant. During a drought, these correlations decreased. The correlation of the flag leaf area with the grain mass of the panicle and plant under stressful conditions of excessive moisture or a drought increased. With excessive moisture, an increase in the proportion of chlorophyll a in the total chlorophyll pool and in the composition of the reaction centers of photosystems was observed in genotypes with an increased number of spikelets and grains in a panicle, with larger grain masses per panicle and plant, and a mass of 1000 grains. During a drought, the correlation of the content of chlorophylls a and b increased with the following signs: panicle length, panicle mass, number of spikelets in a panicle, and number of grains in a panicle. However, an increase in panicle mass and the number of grains in it was accompanied by a decrease in the proportion of chlorophyll a in the total pigment pool and in the composition of reaction centers.

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Effect of Temperature on Sowing Dates of Wheat under Arid and Semi-Arid Climatic Regions and Impact Quantification of Climate Change through Mechanistic Modeling with Evidence from Field

Atmosphere ◽

10.3390/atmos12070927 ◽

2021 ◽

Vol 12 (7) ◽

pp. 927

Author(s):

Jamshad Hussain ◽

Tasneem Khaliq ◽

Muhammad Habib ur Rahman ◽

Asmat Ullah ◽

Ishfaq Ahmed ◽

...

Keyword(s):

Climate Change ◽

Grain Yield ◽

Arid Region ◽

Field Experiments ◽

Climatic Conditions ◽

Seasonal Temperature ◽

Sowing Dates ◽

Rcp 8.5 ◽

Semi Arid Region ◽

Semi Arid

Rising temperature from climate change is the most threatening factor worldwide for crop production. Sustainable wheat production is a challenge due to climate change and variability, which is ultimately a serious threat to food security in Pakistan. A series of field experiments were conducted during seasons 2013–2014 and 2014–2015 in the semi-arid (Faisalabad) and arid (Layyah) regions of Punjab-Pakistan. Three spring wheat genotypes were evaluated under eleven sowing dates from 16 October to 16 March, with an interval of 14–16 days in the two regions. Data for the model calibration and evaluation were collected from field experiments following the standard procedures and protocols. The grain yield under future climate scenarios was simulated by using a well-calibrated CERES-wheat model included in DSSAT v4.7. Future (2051–2100) and baseline (1980–2015) climatic data were simulated using 29 global circulation models (GCMs) under representative concentration pathway (RCP) 8.5. These GCMs were distributed among five quadrants of climatic conditions (Hot/Wet, Hot/Dry, Cool/Dry, Cool/Wet, and Middle) by a stretched distribution approach based on temperature and rainfall change. A maximum of ten GCMs predicted the chances of Middle climatic conditions during the second half of the century (2051–2100). The average temperature during the wheat season in a semi-arid region and arid region would increase by 3.52 °C and 3.84 °C, respectively, under Middle climatic conditions using the RCP 8.5 scenario during the second half-century. The simulated grain yield was reduced by 23.5% in the semi-arid region and 35.45% in the arid region under Middle climatic conditions (scenario). Mean seasonal temperature (MST) of sowing dates ranged from 16 to 27.3 °C, while the mean temperature from the heading to maturity (MTHM) stage was varying between 12.9 to 30.4 °C. Coefficients of determination (R2) between wheat morphology parameters and temperature were highly significant, with a range of 0.84–0.96. Impacts of temperature on wheat sown on 15 March were found to be as severe as to exterminate the crop before heading. The spikes and spikelets were not formed under a mean seasonal temperature higher than 25.5 °C. In a nutshell, elevated temperature (3–4 °C) till the end-century can reduce grain yield by about 30% in semi-arid and arid regions of Pakistan. These findings are crucial for growers and especially for policymakers to decide on sustainable wheat production for food security in the region.

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Improved Rice Technology Adoption: The Role of Spatially-Dependent Risk Preference

Agriculture ◽

10.3390/agriculture11080691 ◽

2021 ◽

Vol 11 (8) ◽

pp. 691

Author(s):

Omotuyole Isiaka Ambali ◽

Francisco Jose Areal ◽

Nikolaos Georgantzis

Keyword(s):

Field Experiments ◽

Climatic Factors ◽

Risk Preferences ◽

Risk Preference ◽

Probit Model ◽

Climatic Conditions ◽

High Yield ◽

Risk Attitudes ◽

Policy Action ◽

Rice Varieties

This study analyses farmers’ adoption of improved rice technology, taking into account farmers’ risk preferences; the unobserved spatial heterogeneity associated with farmers’ risk preferences; farmers’ household and farm characteristics; farm locations, farmers’ access to information, and their perceptions on the rice improved varieties (i.e., high yield varieties, HYV). The study used data obtained from field experiments and a survey conducted in 2016 in Nigeria. An instrumental-variable probit model was estimated to account for potential endogenous farmers’ risk preference in the adoption decision model. Results show that risk averse (risk avoidant) farmers are less likely to adopt HYV, with the spatial lags of farmers’ risk attitudes found to be a good instrument for spatially unobserved variables (e.g., environmental and climatic factors). We conclude that studies supporting policy action aiming at the diffusion of improved rice varieties need to collect information, if possible, on farmers’ risk attitudes, local environmental and climatic conditions (e.g., climatic, topographic, soil quality, pest incidence) in order to ease the design and evaluation of policy actions on the adoption of improved agricultural technology.

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An experiment in the control of the ground water-level in a fen peat soil

The Journal of Agricultural Science ◽

10.1017/s0021859600058639 ◽

1951 ◽

Vol 41 (1-2) ◽

pp. 149-162 ◽

Cited By ~ 3

Author(s):

H. H. Nicholson ◽

G. Alderman ◽

D. H. Firth

Keyword(s):

Ground Water ◽

Water Level ◽

Large Scale ◽

Field Experiments ◽

Peat Soil ◽

Climatic Conditions ◽

Ground Water Level ◽

Effective Control ◽

Soil Conditions ◽

Local Soil

1. The methods of investigation of the effect of ground water-level on crop growth, together with tho field installations in use, are discussed.2. Direct field experiments are handicapped by the difficulties of achieving close control on a sufficiently large scale, due to considerable variations of surface level and depth of peat within individual fields and to rapid fluctuations in rainfall and evaporation. Many recorded experiments are associated with climatic conditions of substantial precipitation during the growing season.3. Seasonal fluctuations of ground water-level in Fen peat soils in England, in natural and agricultural conditions, are described.4. The local soil conditions are outlined and the implications of profile variations are discussed.5. The effective control of ground water-level on a field scale requires deep and commodious ditches and frequent large underdrains to ensure the movement of water underground with sufficient freedom to give rapid compensatory adjustment for marked disturbances of ground water-level following the incidence of heavy rain or excessive evaporation.6. A working installation for a field experiment in ordinary farming conditions is described and the measure of control attained is indicated.

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The use of natural conditions of the high alpine province of Dagestan for reproduction of prospective varieties and hybrids of potato

Kartofel` i ovoshi ◽

10.25630/pav.2021.67.60.001 ◽

2021 ◽

pp. 34-37

Author(s):

В.К. Сердеров ◽

Д.В. Сердерова

Keyword(s):

Field Experiments ◽

Climatic Conditions ◽

High Yield ◽

Potato Varieties ◽

Caspian Lowland ◽

The Republic ◽

High Yielding Varieties ◽

Almost All ◽

The Cost ◽

Mountain Province

Большое значение в увеличении продуктивности картофеля имеет внедрение в производство перспективных высокоурожайных сортов и гибридов, приспособленных к местным природно-климатическим условиям возделывания, от чего зависят эффективность отрасли и себестоимость продукции. Цель работы – изучить влияние климатических условий на продуктивность и качественные показатели сортов и гибридов картофеля, а также использование ими благоприятных почвенно-климатических условий высокогорья Республики Дагестан, для размножения и внедрения в производство новых перспективных, высокоурожайных, приспособленных к условиям среды выращивания сортов картофеля с комплексом хозяйственно-ценных признаков. Объектом изучения послужили сорта картофеля раннего, среднераннего и среднего сроков созревания российской, белорусской и иностранной селекции. Полевые опыты закладывали в 2014–2017 годах на высоте 2000–2200 м, на землях горного опорного пункта «Курахский» Курахского района и на равнинной провинции (Прикаспийской низменности) на территории дачного массива «Наука» Махачкалинского района Республики Дагестан. Схема посадки – 70×30 см, повторность – четырехкратная. Технология выращивания картофеля – гребневая. При проведении полевых опытов использовали общепринятые методики. Практически все сорта картофеля, выращенные на равнинной провинции, сформировали высокий урожай в первый год и при дальнейшем размножении в результате развития вирусных болезней резко снизили свои продуктивные качества. Наиболее высокая урожайность в среднем за три года отмечена у сортов Жуковский ранний (20,8 т/га), Импала (20,5 т/га), Невский (17,8 т/га), Предгорный (16,9 т/га) и Удача (16,3 т/га). В свою очередь, горная провинция Дагестана характеризовалась благоприятными почвенно-климатическими условиями для возделывания как продовольственного картофеля, так и для организации первичного семеноводства на безвирусной основе и размножения перспективных сортов этой культуры. В среднем за годы исследований в горной провинции по урожайности выделились сорта Сильвана (37,4 т/га), Удача (37,6 т/га), Рокко (33,3 т/га), Импала (31,7 т/га), Жуковский ранний (29,0 т/га), Предгорный (27,7 т/га) и Примобелла (27,7 т/га), а также гибриды № 12.40/17 (43,1 т/га) и № 13.61/61 (38,8 т/га). The introduction of promising high-yielding varieties and hybrids adapted to the local natural and climatic conditions of cultivation is of great importance in increasing the productivity of potatoes, which determines the efficiency of the industry and the cost of production. The aim of the work is to study the influence of climatic conditions on the productivity and quality indicators of potato varieties and hybrids, as well as their use of favorable soil and climatic conditions in the highlands of the Republic of Dagestan, for breeding and introducing into production new promising, high-yielding, adapted to the growing environment of potato varieties with a complex of economically valuable characteristics. The object of the study was potato varieties of early, mid-early and medium maturation periods of Russian, Belarusian and foreign selection. Field experiments were conducted in 2014–2017 at an altitude of 2000–2200 m, on the lands of the mountain stronghold Kurakhsky of the Kukhar district and on the plain province (the Caspian lowland) on the territory of the suburban area Nauka of the Makhachkala district of the Republic of Dagestan. The landing scheme is 70×30 cm, the repeatability is four times. The technology of growing potatoes is combed. When conducting field experiments, generally accepted methods were used. Almost all potato varieties grown in the lowland province formed a high yield in the first year and, with further propagation, as a result of the development of viral diseases, sharply reduced their productive qualities. The highest yield on average for three years was observed in the varieties Zhukovskiy ranniy (20.8 t/ha), Impala (20.5 t/ha), Nevsky (17.8 t/ha), Predgornyy (16.9 t/ha) and Udacha (16.3 t/ha). In turn, the mountain province of Dagestan was characterized by favorable soil and climatic conditions for the cultivation of both food potatoes, and for the organization of primary seed production on a virus-free basis and the reproduction of promising varieties of this crop. On average, over the years of research in the mountain province, the varieties Silvana (37.4 t/ha), Udacha (37.6 t/ha), Rocco (33.3 t/ha), Impala (31.7 t/ha), Zhukovskiy ranniy (29.0 t/ha), Predgornyy (27.7 t/ha) and Primobella (27.7 t/ha), as well as hybrids No.12.40/17 (43.1 t/ha) and No.13.61/61 (38.8 t/ha) were distinguished by yield.

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Living mulch strategy for organic cauliflower (Brassica oleracea L.) production in central and southern Italy

Italian Journal of Agronomy ◽

10.4081/ija.2015.644 ◽

2015 ◽

Vol 10 (2) ◽

pp. 90 ◽

Cited By ~ 7

Author(s):

Stefano Canali ◽

Gabriele Campanelli ◽

Corrado Ciaccia ◽

Mariangela Diacono ◽

Fabrizio Leteo ◽

...

Keyword(s):

Brassica Oleracea ◽

Field Experiments ◽

Southern Italy ◽

Central Italy ◽

Climatic Conditions ◽

F1 Hybrid ◽

Agronomic Practices ◽

Burr Medic ◽

The Mean ◽

Sustainable Agricultural Systems

In sustainable agricultural systems, intercropping using living mulches (LM) provides many beneficial ecosystem services. The objective of these two-year field experiments was to study the suitability of different LM options of burr medic (Medicago polymorpha L. var. anglona) for organic cauliflower (Brassica oleracea L.) cultivation in two sites under Mediterranean conditions. In central Italy (Experiment 1) contemporary and delayed (to crop) sowings of LM were compared with a no-cover crop treatment, contrasting two local cauliflower cultivars and a F1 Hybrid. In southern Italy (Experiment 2) the sustainability of systems combining LM (anticipated and contemporary sowing compared with no-cover) and organic fertilisation strategies was assessed. The aboveground biomasses dry weights of cauliflower crop (heads and residues), burr medic and weeds were separately determined. Results suggested that in Experiment 1 the LM was not able to smother weeds establishment and growth, as a consequence of early sowing, while cauliflower yield was reduced. Moreover, the genotypes behaviour was greatly influenced by the LM sowing times. In Experiment 2, irrespective of the agronomic practices applied, climatic conditions notably influenced cauliflower cultivation and also reduced the mean yield. Therefore, the recorded differences between the two experimental sites highlighted the need to tailor the LM strategies to the different environmental conditions.

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Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings

Zeitschrift für Naturforschung C ◽

10.1515/znc-1981-5-614 ◽

1981 ◽

Vol 36 (5-6) ◽

pp. 421-430 ◽

Cited By ~ 37

Author(s):

H. K. Lichtenthaler ◽

G. Burkard ◽

G. Kuhn ◽

U. Prenzel

Keyword(s):

Chlorophyll A ◽

Early Stage ◽

Continuous Darkness ◽

Suitable Parameter ◽

Chlorophyll Accumulation ◽

Chlorophylls A And B ◽

Sds Page ◽

Chlorophyll Protein ◽

Chlorophyll A And B ◽

Radish Seedlings

Abstract Illumination of 3 day old etiolated radish seedlings with continuous white light results in a progressive accumulation of chlorophyll a and b. Both pigments are bound in a different way to the thylakoid chlorophyll-proteins, which appear parallel to the formation of chlorophylls. By applying the SDS-PAGE method to SDS-digested chloroplasts, it was possible to show that the chloroplasts of radish cotyledons contain the typical chlorophyll proteins LHCP1-3, CPa, CPI and CPIa which have been found in other plants. Between LHCP1 and CPI an additional chlorophyll protein is detected with the spectral properties of a LHCP; it is termed here LHCPy. When the green plants are transferred to continuous darkness, chlorophylls and the chlorophyll-proteins are progressively degraded. At an early stage of greening chlorophyll b is destroyed at a much higher rate in darkness than chlorophyll a, which yields high chlorophyll a/b ratios. This is paralleled by a faster decrease in the level of the corresponding chlorophyll a/b-protein LHCP3 than of CPI. At a later stage of greening, after the end of the logarithmic chlorophyll accumulation, the chlorophylls a and b and also the LHCP3 and CPI are destroyed in continuous darkness at equal rates; the a/b ratios and the LHCP3/CPI ratios are then little different from the light control. The data indicate that at an early stage of greening the light-harvesting chlorophyll a/b-protein LHCP3 is less stable than the other chlorophyll-proteins (CPI, CPIa, CPa), which contain pre dominantly chlorophyll a. The ratio chlorophyll a to β-carotene (a/c ratio) of CPIa, CPI and CPa is about 10, while that of the LHCP1-3 is found to be between 150 to 300. We therefore propose using the a/c ratio to define the chlorophyll-proteins which, besides the absorption spectra, is the most suitable parameter.

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Simulating soil organic C dynamics in managed grasslands under humid temperate climatic conditions

10.5194/soil-2020-76 ◽

2020 ◽

Author(s):

Asma Jebari ◽

Jorge Álvaro-Fuentes ◽

Guillermo Pardo ◽

María Almagro ◽

Agustin del Prado

Keyword(s):

Field Experiments ◽

Model Performance ◽

Climatic Conditions ◽

Plant Residue ◽

Plant Residues ◽

Organic C ◽

Natural Grasslands ◽

Rothc Model ◽

Below Ground ◽

Intensively Managed

Abstract. Temperate grasslands are of paramount importance in terms of soil organic carbon (SOC) dynamics. Globally, research on SOC dynamics has largely focused on forests, croplands and natural grasslands, while intensively managed grasslands has received much less attention. In this regard, we aimed to improve the prediction of SOC dynamics in managed grasslands under humid temperate regions. In order to do so, we modified and recalibrated the SOC model RothC, originally developed to model the turnover of SOC in arable topsoils, which requires limited amount of readily available input data. The modifications proposed for the RothC are: (1) water content up to saturation conditions in the soil water function of RothC to fit the humid temperate climatic conditions, (2) entry pools that account for particularity of exogenous organic matter (EOM) applied (e.g., ruminant excreta), (3) annual variation in the carbon inputs derived from plant residues considering both above- and below-ground plant residue and rhizodeposits components as well as their quality, and (4) the livestock treading effect (i.e., poaching damage) as a common problem in humid areas with higher annual precipitation. In the paper, we describe the basis of these modifications, carry out a simple sensitivity analysis and validate predictions against data from existing field experiments from four sites in Europe. Model performance showed that modified RothC reasonably captures well the different modifications. However, the model seems to be more sensitive to soil moisture and plant residues modifications than to the other modifications. The applied changes in RothC model could be appropriate to simulate both farm and regional SOC dynamics from managed grassland-based systems under humid temperate conditions.

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Variability assessment of productivity traits and quality of berries in the genetic collection of garden strawberry (Fragaria ananassa Duch.) in the Orenburg region

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/845/1/012015 ◽

2021 ◽

Vol 845 (1) ◽

pp. 012015

Author(s):

E V Aminova ◽

R R Salimova ◽

O E Merezhko

Keyword(s):

Field Experiments ◽

Sugar Content ◽

Climatic Conditions ◽

Soluble Solids ◽

Average Weight ◽

Marketable Yield ◽

Berry Quality ◽

Growing Conditions ◽

Productivity Potential

Abstract Nowadays the study of the interrelation of the genotype of strawberry plants in garden and climatic conditions is extremely relevant. Due to the various systems of genetic control and the modifying effects of growing conditions on the manifestation of quantitative traits, there is the need to assess the genotypic variability of economically valuable features, focused on the identification of genotypes characterized by stability and adaptive qualities in growing conditions. The study examined 15 varieties of garden strawberries of domestic and foreign selection. The field experiments and surveys were carried out according to the Program generally accepted in the Russian Federation and methodology for the variety study of fruit, berry and nut crops. We studied such features as the number of peduncles (pcs/bush), number of berries (pcs/bush), average weight of berries (g), total and marketable yield (g/bush), sugar content in berries, soluble solids and ascorbic acid. The purpose of this work was to assess the genotype-year interrelation in terms of the variability of productivity features and berry quality and to identify strawberry varieties with a stable genotype. As a result of two-way analysis of variance for the variety-year interrelation, the obtained values were 1.10-8.50 at standard Fst. - 1.24. Statistically important differences had indicators of productivity of a bush between the first and second clusters (t = 5.89 at p <0.01), the first and third (t = 15.83 at p <0.01), the second and third clusters (t = 8.13 at p <0.01), as well as the average berry weight between the first and third, second and third clusters (t = 15.50 and 6.99 at p <0.01, respectively). Significant differences in the value of the Euclidean distance were revealed for varieties Mishutka (54.5), Daryonka (54.5), Pervoklassnitsa (58) realizing their productivity potential in different years of cultivation.

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Fertilization of Algal Resources By an Exotic Snail May Facilitate Invasion

The UW National Parks Service Research Station Annual Reports ◽

10.13001/uwnpsrc.2003.3555 ◽

2003 ◽

Vol 27 ◽

pp. 116-122

Author(s):

Leslie Riley ◽

Mark Dybdahl ◽

Robert Hall

Keyword(s):

Chlorophyll A ◽

Field Experiments ◽

Gross Primary Production ◽

Nutrient Recycling ◽

Algal Growth ◽

Grazing Pressure ◽

Nitrogen Excretion ◽

Macroinvertebrate Communities ◽

Negative Effects ◽

The Difference

We studied positive and negative effects of snail consumers on their resource to determine if positive consumer effects may be facilitating invasion. Consumer- resource interactions often focus on losses to the resource, even though the resource might benefit if consumers recycle nutrients. The New Zealand mudsnail, Potamopyrgus antipodarum, an exotic in western U.S. rivers, attains high densities and dominates macroinvertebrate communities. In one well-studied river, it consumes the majority of primary productivity, cycles most nitrogen and can grow faster at higher densities. In field experiments, we tested the hypothesis that this invasive grazer stimulates algal growth via nitrogen excretion, which might explain its self-facilitation and invasiveness. Using in-stream cages subdivided into "with snails" and "without snails" sections, we examined the response of periphytic algae to snail grazing and excretion and snail excretion alone at various levels of snail biomass. We found that chlorophyll a and GPP (gross primary production) decreased as the biomass of snails increased in the grazed sections. Snail excretion, in the absence of grazing, increased both chlorophyll a and GPP, demonstrating a positive effect of snails on the resource, consistent with the nutrient recycling and enrichment hypothesis. We found no evidence for increased algal growth at intermediate snail densities in grazed treatments, as predicted by the Herbivore Optimization Curve hypothesis. However, the difference in chlorophyll a between "with snails" and "without snails" treatments increased as snail biomass increased. This suggests that snail compensation of the resource, through excretion, decreases at extremely high levels of grazing pressure and the net effect of snail grazing becomes negative. Together, these results suggest that invasiveness in some rivers may be fostered by this self-facilitation and recycling of essential nutrients.

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Chlorophyll Content in Leaves of Wheat as Influenced by Inorganic, Organic and Integrated Sources of Nutrient Application

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2021/v33i230412 ◽

2021 ◽

pp. 31-45

Author(s):

Megha Vishwakarma ◽

P. S. Kulhare ◽

G. S. Tagore

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Field Experiments ◽

Winter Season ◽

Leaf Tissue ◽

Coefficient Of Determination ◽

Growth Stages ◽

Total Chlorophyll ◽

Pooled Data ◽

Spad Value

Field experiments were conducted during winter season of 2018-19 and 2019-20 with three sources of nutrient viz., inorganic, organics (FYM, VC and biofertilizers) and their integration as main treatments and five levels [S1-0 (0-0-0 kg NPK ha-1), S2-100% (120-60-40 kg NPK ha-1), S3-150% (180-90-60 kg NPK ha-1), S4-200% (240-120-80 kg NPK ha-1) and S5-Soil Test based (STV) NPK i.e. 149-176-33 kg ha-1 in split plot design with three replications. The chlorophyll content (‘a’, ‘b’ and total) in leaves and Soil Plant Analyzer Development (SPAD) value were recorded at crown root initiation (CRI), tillering, jointing and milking stage of wheat. The pooled data of findings revealed that the treatment with inorganic sources showed significant increase in the SPAD readings (9.62, 15.54, 23.77 and 29.83), chlorophyll ‘a’ (0.76, 0.83, 1.47 and 0.63 mg g-1 leaf tissue), ‘b’ (0.44, 0.78, 0.87 and 0.57 mg g-1 leaf tissue) and total (1.19, 1.64, 2.25 and 1.14 mg g-1 leaf tissue) chlorophyll content in leaves over organic source at all the growth stages. All the levels of nutrient were significantly increased the chlorophyll content and SPAD value over control at all the stages except chlorophyll ‘a’ at jointing and milking stage. However, amongst the levels 150% and 200% NPK were found significantly superior to 100% NPK for SPAD value (8.32 and 8.71 at CRI and 12.56 and 12.19 at tillering), chlorophyll ‘a’ (0.73 and 0.70 mg g-1 leaf tissue at CRI), chlorophyll ‘b’ (0.46 and 0.45 mg g-1 leaf tissue at CRI, 0.68 and 0.71 mg g-1 leaf tissue at tillering and 0.53 and 0.59 mg g-1 leaf tissue at milking), respectively. The interaction results suggested that the 200% NPK with inorganic and integrated sources significantly superior to 100% NPK for chlorophyll ‘a’ content at jointing and milking stage. The application of 150% and 200% NPK with inorganic source were found significantly higher over the same level of NPK with integrated source of nutrient for total chlorophyll content and SPAD value at all the growth stages except 150% NPK for total chlorophyll at jointing and milking stage and SPAD value at milking stage. The correlation between SPAD value and chlorophyll ’a’, ‘b’, total were found significantly and positively at all growth stages. Coefficient of determination values between SPAD and chlorophyll content showed linear relationship at all the growth stages.

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