HUBUNGAN SIKAP IBU YANG MEMPUNYAI BAYI DENGAN PELAKSANAAN IMUNISASI DPT DI PUSKESMAS KAWALI KABUPATEN CIAMIS

Kendala utama keberhasilan vaksinasi bayi dan anak dalam sistem kesehatan adalah pengetahuan yang rendah dan kurangnya kebutuhan vaksinasi masyarakat. Sikap ibu terhadap vaksinasi akan menimbulkan perilaku yang mendukung terhadap kegiatan vaksinasi. Ini merupakan faktor dominan keberhasilan vaksinasi, jika sikap positif diharapkan pelaksanaan vaksinasi meningkat. Sikap ibu meningkatkan kepercayaan ibu terhadap kesehatan dan mempengaruhi status vaksinasi bayi dan balita. TUJUAN: Penelitian ini bertujuan untuk mengetahui hubungan antara sikap ibu yang memiliki bayi terhadap pemberian vaksin DPT. Metode penelitian yang digunakan penelitian korelasional dengan desain penelitian cross sectional. Sampel yang digunakan sebanyak 73 orang ibu yang memiliki anak balita dengan teknik total sampling. Hasil penelitian menunjukkan bahwa sebanyak 11 orang (15,1%) memiliki sikap negatif, 2 responden memiliki sikap negatif (2,7%) tetapi telah mendapatkan vaksin dan sebanyak 62 orang (84,9%) memiliki sikap positif terhadap vaksinasi. Analisis bivariat dengan nilai P & lt; a (0,001 < 0,05). Jadi Ha diterima dan Ho ditolak. Kesimpulan: Kesimpulan dari penelitian ini adalah ada hubungan antara sikap ibu yang memiliki bayi dengan penerapan vaksin DPT.The main obstacle to the success of infant and child vaccination in the health system is the low level of knowledge and the lack of community vaccination needs. Mother's attitude towards vaccination will lead to supportive behavior towards vaccination activities. This is a dominant factor in the success of vaccination, if a positive attitude is expected to increase vaccination implementation. Mother's attitude increases maternal confidence in health and affects the vaccination status of infants and toddlers. OBJECTIVE: This study aims to determine the relationship between the attitudes of mothers who have babies to the DPT vaccine. The research method used was correlational research with a cross sectional research design. The sample used as many as 73 mothers who have children under five with total sampling technique. The results showed that as many as 11 people (15.1%) had a negative attitude, 2 respondents had a negative attitude (2.7%) but had received the vaccine and as many as 62 people (84.9%) had a positive attitude towards vaccination. Bivariate analysis with P value & lt; a (0.001 < 0.05). So Ha is accepted and Ho is rejected. Conclusion: The conclusion of this study is that there is a relationship between the attitude of mothers who have babies with the application of the DPT vaccine.

Draft Genome Sequences of Corynebacterium diphtheriae Clinical Isolates from Colombia

Since the implementation of the diphtheria-tetanus-pertussis (DPT) vaccine in Colombia, there has been a decrease in the reporting of cases. Here, we report two isolates of Corynebacterium diphtheriae bv. mitis isolated in the reference laboratory at Instituto Nacional de Salud from samples received from Norte de Santander and La Guajira; both areas are located on the northeast border of Colombia.

ANTIPYRETIC ACTIVITY OF CENTELLA (CENTELLA ASIATICA (L.) URBAN) HERB IN VACCINE INDUCED MICE

Objective: This study aimed to determine the antipyretic activity of the infusion of centella (Centella asiatica (L.) Urban) herb, based on empirically usage of Indonesian people. Methods: Antipyretic activity assay was conducted to Swiss-Webster mice, which induced by diphtheria-pertussis-tetanus (DPT) vaccine with paracetamol as positive control. Results: Centella herb was contained tannins, flavonoids, monoterpenoids, sesquiterpenoids, triterpenoids, steroids, and saponins. The antipyretic activity of centella infusion has a ratio 1:44.6, compare to paracetamol. Conclusion: Antipyretic activity of centella herbs was predicted due to its secondary metabolites.

UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

UJI EFEK ANTIPIRETIK EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.)

ABSTRACTStarfruit leaves (Averrhoa Bilimbi L) is a plant that contains tannins and flavonoids. In accordance with previous research it was stated that tannins and flavonoids have antiperetic activity. This study aims to determine whether the ethanol extract of starfruit leaves has an antiperetic effect in wistar male rats (Rattus Norvegicus L) induced by the DPT vaccine. This study used 15 test animals and devided into 5 grops consisting of negative control (cmc 1%), positive control (paracetamol) and the test dose group namely the administration of starfruit leaves extract 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. Each mouse was induced with DPT vaccine doses of 0,2 ml intramuscularly. Temperature measurements were made taken before induction, 2 hours after induction and every 30 minutes for 3 hours. The results showed a decrease in temperature in the 60th minute of starfruit leaves 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. The data obtained were analyzed using one-way ANOVA test and LSD test. Based on the results of the study it can be concluded that the ethanol extract of starfruit leaves 3,6 m, 7,2 mg and 14,4 mg has antiperetic activity in male white rats. Keywords : Averrhoa bilimbi L,Antiperetic,Rattus novergicus L,Vaksin DPTABSTRAK Daun belimbing wuluh (Averrhoa bilimbi L.) adalah salah satu tanaman yang mengandung zat tanin dan flavonoid. Berdasarkan penelitian-penelitian sebelumnya menyatakan bahwa tanin dan flavonoid mempunyai aktivitas antipiretik. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun belimbing wuluh memiliki efek sebagai antipiretik pada tikus putih jantan (Rattus norvegicus L.) yang di induksikan vaksin DPT. Penelitian ini menggunakan hewan uji sebanyak 15 ekor dan dibagi menjadi 5 kelompok yang terdiri dari kontrol negatif (CMC 1%), kontrol positif (parasetamol) dan kelompok dosis uji yaitu pemberian ekstrak daun Belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram BB. Tiap tikus diinduksi dengan vaksin DPT sebanyak 0,2 ml secara intramuskular. Pengukuran suhu dilakukan sebelum induksi, 2 jam setelah pemberian induksi dan setiap 30 menit selama 3 jam. Hasil penelitian menunjukkan terjadi penuruan suhu pada menit yang ke 60 ekstrak etanol daun belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram BB. Data yang diperoleh dianalisis dengan menggunakan uji ANOVA satu arah dan uji LSD dapat menurunkan suhu rektal tikus. Data yang diperoleh dianalisa menggunakan ANOVA. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol daun belimbing wuluh 3,6 mg, 7,2 mg, dan 14,4 mg mempunyai aktivitas antipiretik pada tikus putih jantan. Kata Kunci : Averrhoa bilimbi L., Antipiretik, Rattus norvegicus L, Vaksin DPT

Child with diphtheria pertussis and tetanus induced afebrile seizures

Current childhood vaccines are safe and effective but diphtheria pertussis and tetanus vaccine (DPT) has been considered to be one of the most reactogenic. The adverse reactions after immunization with adsorbed DPT vaccine have been widely debated and publicized. The conventional whole cell pertussis vaccines expected to give certain minor and local reactions as it contains lipopolysaccharide and active pertussis toxin (PT). Reactions occurring after DPT vaccine have been described mostly to the pertussis component of the vaccine. Vaccination with diphtheria and tetanus toxoids and whole cell pertussis, DPT vaccine was associated with a risk of seizures and encephalopathy.

Plectranthus scutellarioides (L.) Reduces the Rectal Temperature of Diphteria-Pertussis-Tetanus Vaccine-Induced Mice

The pharmacological activities of Plecranthus sp include anti-oxidant, antimicrobial, inhibition on cyclooxygenase (COX) and xanthine oxidase, and cytotoxic activity. The aim of this work was to study the antipyretic activity of P. scutellarioides (L.) extract on animal model. Twenty seven Swiss Webster male mice were divided into nine groups and were pyrexia-induced by using 0.1 ml of diphteria-pertussis-tetanus (DPT) vaccine. Their body temperature was measured every 60 minutes. After 4 hours of DPT vaccine injection, the mice were treated with (I) Arabic gum suspension (PGA) 2%; (II) paracetamol 16.25 mg/kg BW; (III) paracetamol 32.5 mg/kg BW; (IV) paracetamol 65 mg/kg BW; (V) paracetamol 130 mg/kg BW; (VI) P. scutellarioides extract 70 mg/kg BW; (VII) P. scutellarioides extract 140 mg/kg BW; (VIII) P. scutellarioides extract 280 mg/kg BW; (IX) P. scutellarioides extract 560 mg/kg BW. Data were analyzed using SPSS 21. Result showed that the body temperature of the mice started to rise at 2.5 hours and reached its peak at 4 hours after vaccine induction. The P. scutellarioides extract dose 280 mg/kg of BW indicated an 80.47% decrease (α= 0.05) compared to paracetamol dose 130 mg/kg of BW which decreases 85.43% of the animals’ fever. Interestingly, higher dose of the extract indicated a decrease in the antipyretic effect. We concluded that P. scutellarioides might possess antipyretic activity which started as early as 5 hours post vaccine-induced and was maintained for 3 hours. This plant needs further scientific exploration Keywords: Diphteria-pertusis-tetanus vaccine, anti-pyretic, rectal temperature, Webster mice

The Comparison of the adverse events of Pentavalent and DPT vaccines in 2-6 months infants in Iran: A national study

Background Vaccination is the most remarkable intervention in public health and is an effective strategy in order to control infectious diseases among infants. This study attempted to compare the adverse events of Pentavalent and DPT vaccines together. Methods This is a cross sectional (analytical-descriptive) study in which the 2-6 months healthy infants, having received DPT in 2013 and Pentavalent in 2015, were studied for their experienced adverse events related to these vaccines. Percentage, mean, standard deviation and chi-square tests were used to describe and analyze the data. Results The results showed that 10464 and 17561 adverse events, which are associated with DPT and Pentavalent respectively, were recorded in the infants who received these vaccines throughout Iran. Mazandaran, Qazvin and Golestan provinces reported the highest number of adverse events, respectively (15.74%, 11.25%, and 9.12%). Moreover, Pentavalent seemed to have more recorded adverse events compared to DPT, high fever had the highest record rate for DPT (47.4%) and mild localized complications was the highest for Pentavalent (31.68%). There was a significant relationship between the kind of vaccine and the type of reaction, adverse event categorization, the country produced the vaccine and the infants' place of residence (p<0.05). Conclusion Severe localized adverse events including high fever, vomiting, diarrhea and restlessness seemed to be less in Pentavalent compared to DPT vaccine. Therefore, substituting Pentavalent for DPT in infants seems to reduce the adverse events among them.