Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding

Narendar K. Khatri; Dörte Gocke; Oliver Trentmann; Peter Neubauer; Frank Hoffmann

doi:10.1002/biot.201000193

Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding

Biotechnology Journal ◽

10.1002/biot.201000193 ◽

2011 ◽

Vol 6 (4) ◽

pp. 452-462 ◽

Cited By ~ 10

Author(s):

Narendar K. Khatri ◽

Dörte Gocke ◽

Oliver Trentmann ◽

Peter Neubauer ◽

Frank Hoffmann

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment ◽

Fragment Production ◽

Glycerol Feeding

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Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris

Applied Microbiology and Biotechnology ◽

10.1007/s00253-005-0306-1 ◽

2006 ◽

Vol 72 (3) ◽

pp. 492-498 ◽

Cited By ~ 27

Author(s):

Narendar K. Khatri ◽

Frank Hoffmann

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Recombinant Pichia Pastoris ◽

Single Chain Antibody Fragment

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Hybrid metabolic flux analysis and recombinant protein prediction in Pichia pastoris X-33 cultures expressing a single-chain antibody fragment

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-016-1611-z ◽

2016 ◽

Vol 39 (9) ◽

pp. 1351-1363 ◽

Cited By ~ 9

Author(s):

Inês A. Isidro ◽

Rui M. Portela ◽

João J. Clemente ◽

António E. Cunha ◽

Rui Oliveira

Keyword(s):

Pichia Pastoris ◽

Recombinant Protein ◽

Metabolic Flux Analysis ◽

Metabolic Flux ◽

Antibody Fragment ◽

Flux Analysis ◽

Single Chain ◽

Single Chain Antibody ◽

Protein Prediction ◽

Single Chain Antibody Fragment

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High-level production in Pichia pastoris of an anti-p185HER-2 single-chain antibody fragment using an alternative secretion expression vector

Biotechnology and Applied Biochemistry ◽

10.1042/ba20030096 ◽

2004 ◽

Vol 39 (1) ◽

pp. 115 ◽

Cited By ~ 14

Author(s):

Cemal Gurkan ◽

Stefan N. Symeonides ◽

David J. Ellar

Keyword(s):

Pichia Pastoris ◽

Expression Vector ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Secretion Expression ◽

High Level ◽

Single Chain Antibody Fragment ◽

Level Production

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Application of adaptive DO-stat feeding control to Pichia pastoris X33 cultures expressing a single chain antibody fragment (scFv)

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-012-0751-z ◽

2012 ◽

Vol 35 (9) ◽

pp. 1603-1614 ◽

Cited By ~ 14

Author(s):

A. R. Ferreira ◽

F. Ataíde ◽

M. von Stosch ◽

J. M. L. Dias ◽

J. J. Clemente ◽

...

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Feeding Control ◽

Single Chain Antibody Fragment

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Aqueous micellar systems containing Triton X-114 and Pichia pastoris fermentation supernatant: A novel alternative for single chain-antibody fragment purification

Separation and Purification Technology ◽

10.1016/j.seppur.2014.05.045 ◽

2014 ◽

Vol 132 ◽

pp. 295-301 ◽

Cited By ~ 11

Author(s):

Luciana P. Malpiedi ◽

Bibiana B. Nerli ◽

Dulcineia S.P. Abdala ◽

Pedro de Alcântara Pessôa-Filho ◽

Adalberto Pessoa

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Micellar Systems ◽

Triton X ◽

Single Chain Antibody Fragment

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Production of a single chain antibody fragment under oxygen limited conditions by Pichia pastoris

New Biotechnology ◽

10.1016/j.nbt.2009.06.214 ◽

2009 ◽

Vol 25 ◽

pp. S233

Author(s):

N.K. Khatri ◽

F. Hoffmann ◽

P. Neubauer

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

Applied Microbiology and Biotechnology ◽

10.1007/s00253-006-0652-7 ◽

2007 ◽

Vol 74 (2) ◽

pp. 381-389 ◽

Cited By ~ 78

Author(s):

Leonardo M. Damasceno ◽

Kyle A. Anderson ◽

Gerd Ritter ◽

James M. Cregg ◽

Lloyd J. Old ◽

...

Keyword(s):

Pichia Pastoris ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Enhanced Secretion ◽

Single Chain Antibody Fragment

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Production of an Anti-Prostate-Specific Antigen Single-Chain Antibody Fragment from Pichia pastoris

Protein Expression and Purification ◽

10.1006/prep.2001.1521 ◽

2001 ◽

Vol 23 (3) ◽

pp. 419-425 ◽

Cited By ~ 10

Author(s):

Yunjun Wang ◽

Kathy Wang ◽

Diane C. Jette ◽

David S. Wishart

Keyword(s):

Pichia Pastoris ◽

Prostate Specific Antigen ◽

Specific Antigen ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Brolucizumab: a novel anti-VEGF humanized single-chain antibody fragment for treating w-AMD

Expert Opinion on Biological Therapy ◽

10.1080/14712598.2021.1915278 ◽

2021 ◽

Vol 21 (5) ◽

pp. 553-561

Author(s):

Lorenzo Ferro Desideri ◽

Carlo Enrico Traverso ◽

Massimo Nicolò

Keyword(s):

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Anti Vegf ◽

Single Chain Antibody Fragment

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423. Isolation of an Adeno-Associated Virus (AAV)-Specific Camelid-Derived Single Chain Antibody Fragment: A Novel Tool for Purification of AAV Vectors of Different Serotypes

Molecular Therapy ◽

10.1016/j.ymthe.2004.06.344 ◽

2004 ◽

Vol 9 ◽

pp. S162 ◽

Cited By ~ 2

Keyword(s):

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Adeno Associated Virus ◽

Single Chain Antibody Fragment

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