High-level production in Pichia pastoris of an anti-p185HER-2 single-chain antibody fragment using an alternative secretion expression vector

2004 ◽  
Vol 39 (1) ◽  
pp. 115 ◽  
Author(s):  
Cemal Gurkan ◽  
Stefan N. Symeonides ◽  
David J. Ellar
Keyword(s):  
Pichia Pastoris ◽  
Expression Vector ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Secretion Expression ◽  
High Level ◽  
Single Chain Antibody Fragment ◽  
Level Production

An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris

2004 ◽  
Vol 37 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Leonardo M Damasceno ◽  
Itzcoatl Pla ◽  
Hyun-Joo Chang ◽  
Leonard Cohen ◽  
Gerd Ritter ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Fermentation Process ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
High Level ◽  
Level Production ◽  
Single Chain Fv Antibody

Application of adaptive DO-stat feeding control to Pichia pastoris X33 cultures expressing a single chain antibody fragment (scFv)

2012 ◽  
Vol 35 (9) ◽  
pp. 1603-1614 ◽  
Author(s):  
A. R. Ferreira ◽  
F. Ataíde ◽  
M. von Stosch ◽  
J. M. L. Dias ◽  
J. J. Clemente ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Feeding Control ◽  
Single Chain Antibody Fragment

Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding

2011 ◽  
Vol 6 (4) ◽  
pp. 452-462 ◽  
Author(s):  
Narendar K. Khatri ◽  
Dörte Gocke ◽  
Oliver Trentmann ◽  
Peter Neubauer ◽  
Frank Hoffmann
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment ◽  
Fragment Production ◽  
Glycerol Feeding

Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

2007 ◽  
Vol 74 (2) ◽  
pp. 381-389 ◽  
Author(s):  
Leonardo M. Damasceno ◽  
Kyle A. Anderson ◽  
Gerd Ritter ◽  
James M. Cregg ◽  
Lloyd J. Old ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Enhanced Secretion ◽  
Single Chain Antibody Fragment
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