ChemInform Abstract: IMPROVED DEPROTECTION IN SOLID PHASE PEPTIDE SYNTHESIS: REMOVAL OF PROTECTING GROUPS FROM SYNTHETIC PEPTIDES BY AN SN2 MECHANISM WITH LOW CONCENTRATIONS OF HYDROGEN FLUORIDE IN DIMETHYL SULFIDE

1983 ◽  
Vol 14 (8) ◽  
Author(s):  
J. P. TAM ◽  
W. F. HEATH ◽  
R. B. MERRIFIELD
Keyword(s):  
Peptide Synthesis ◽  
Hydrogen Fluoride ◽  
Synthetic Peptides ◽  
Dimethyl Sulfide ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Protecting Groups ◽  
Low Concentrations

scholarly journals Design and Validation of a Frugal, Automated, Solid-Phase Peptide Synthesizer

2020 ◽  
Author(s):  
Nathaniel E. Kallmyer ◽  
Nathan E. Rider ◽  
Nigel F. Reuel
Keyword(s):  
Peptide Synthesis ◽  
Self Assembly ◽  
Synthetic Peptides ◽  
Materials Science ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Fluid Handling ◽  
Price Point ◽  
Grade Fluid ◽  
Chemistry Laboratories

AbstractSolid phase peptide synthesis (SPPS) has enabled widespread use of synthetic peptides in applications ranging from pharmaceuticals to materials science. The demand for synthetic peptides has driven recent efforts to produce automated SPPS synthesizers which utilize fluid-handling components common to chemistry laboratories to drive costs down to several thousand dollars. Herein, we describe the design and validation of a more ‘frugal’ SPPS synthesizer that uses inexpensive, consumer-grade fluid-handling components to achieve a prototype price point between US$300 and $600. We demonstrated functionality by preparing and characterizing peptides with a variety of distinct properties including binding functionality, nanoscale self-assembly, and oxidation-induced fluorescence. This system yielded micromoles of peptide at a cost of approximately $1/residue, a cost which may be further reduced by optimization and bulk purchasing.

scholarly journals Role of capping in peptide synthesis

2020 ◽  
Vol 11 (4) ◽  
pp. 5225-5228
Author(s):  
Deepshikha Verma ◽  
Pillai V N R ◽  
Giriraj Tailor
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Protecting Groups ◽  
Amino Groups ◽  
Peptide Chemistry ◽  
Fmoc Spps ◽  
Reliable Methods ◽  
Important Test

Protecting groups like Fmoc and coupling both steps are essential to monitoring the Fmoc SPPS (Solid Phase Peptide Synthesis) reaction completion. Reliable methods are used to detect the unreacted number of amino groups for monitoring these two essential reaction steps of coupling and cleavage. The ability to detect the complete coupling, incomplete coupling or failure of coupling we use many colour tests in the laboratory and based on this the Fmoc peptide chemistry allows the control of the completion of the Fmoc cleavage. The most important test used is the Kaiser test and highly recommended to monitor the coupling and cleavage steps. If the result of colour tests is positive after coupling, then the second coupling should be performed. Then again use the colour test to detect the level of coupling. If the result is still slightly positive, repeat coupling with the smaller modification of reagents such as used PyBOP instead of HOBT AND HOAT. These colour tests help in revealing the presence of unreacted amino-functional groups. Thus, we need to block these free N-terminal of amino- acids which help in avoiding the making of deletion of sequence.

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