Supercritical fluid extraction of amino acids from birch (Betula pendula Roth) leaves and their liquid chromatographic determination with fluorimetric detection

2008 ◽  
Vol 31 (8) ◽  
pp. 1363-1373 ◽  
Author(s):  
Bořivoj Klejdus ◽  
Lea Lojková ◽  
Emanuel Kula ◽  
Ivan Buchta ◽  
Petr Hrdlička ◽  
...  
Keyword(s):  
Amino Acids ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Betula Pendula ◽  
Chromatographic Determination ◽  
Fluorimetric Detection ◽  
Fluid Extraction ◽  
Betula Pendula Roth ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Reversed-Phase Liquid Chromatographic Determination of Hypoglycin A (HG-A) in Canned Ackee Fruit Samples

1994 ◽  
Vol 77 (5) ◽  
pp. 1175-1179 ◽  
Author(s):  
Ghulam Sarwar ◽  
Herbert G Botting
Keyword(s):  
Amino Acids ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Precolumn Derivatization ◽  
Edible Portion ◽  
Fruit Samples ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) method involving precolumn derivatization with phenylisothiocyanate (PITC) was developed for determining levels of hypoglycin A (HG-A) in canned ackee fruit samples. HG-A was extracted by homogenizing the drained fruit in 80% ethanol. By using a Waters Pico-Tag amino acid analysis 15-cm-long column (which is also used for analyzing protein hydrolysates and biological samples) and an LC system, the baseline separation of HG-A from other amino acids was completed in about 6 min. The total time for analysis and equilibration was 16 min. HG-A levels in the edible portion of fruit in 18 cans varied from 18.27 to 87.50 mg HG-A/can. Recoveries of added standard HG-A averaged 101%. To our knowledge, this is the first report of the use of this method to determine HG-A in ackee fruit.

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